Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (6): 902-910.DOI: 10.1016/S1671-2927(11)60075-8

• 论文 • 上一篇    下一篇

Differential Gene and Protein Expression in Soybean at Early Stages of Incompatible Interaction with Phytophthora sojae

LI Yong-gang1, YANG Ming-xiu1, LI Yan1, LIU Wen-wen1, WEN Jing-zhi1 and LI Yong-hao2   

  1.  1 Department of Plant Protection, Northeast Agricultural University, Harbin 150030, P.R.China
     2 Department of Plant Pathology and Ecology, Connecticut Agricultural Experiment Station, 123 Huntington St. New Haven, CT 06511, USA
  • 收稿日期:2011-06-10 修回日期:2011-06-10 出版日期:2011-06-20 发布日期:2011-06-03
  • 通讯作者: WEN Jing-zhi
  • 作者简介:LI Yong-gang, Ph D, E-mail: neaulyg@yahoo.cn; Correspondence WEN Jing-zhi, Professor, Ph D, Tel: +86-451-55191045, Fax: +86-451-55191045, E-mail: jzhwen2000@yahoo.com.cn

Differential Gene and Protein Expression in Soybean at Early Stages of Incompatible Interaction with Phytophthora sojae

LI Yong-gang1, YANG Ming-xiu1, LI Yan1, LIU Wen-wen1, WEN Jing-zhi1 and LI Yong-hao2   

  1. 1 Department of Plant Protection, Northeast Agricultural University, Harbin 150030, P.R.China 
    2 Department of Plant Pathology and Ecology, Connecticut Agricultural Experiment Station, 123 Huntington St. New Haven, CT 06511, USA
  • Received:2011-06-10 Revised:2011-06-10 Online:2011-06-20 Published:2011-06-03
  • Contact: WEN Jing-zhi
  • About author:LI Yong-gang, Ph D, E-mail: neaulyg@yahoo.cn; Correspondence WEN Jing-zhi, Professor, Ph D, Tel: +86-451-55191045, Fax: +86-451-55191045, E-mail: jzhwen2000@yahoo.com.cn

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摘要： Soybean root and stem rot caused by Phytophthora sojae is a destructive disease worldwide. Using genetic resistance is an important and major component in the integrated pest management of this disease. To understand molecular mechanisms of root and stem rot resistance in soybeans, the gene and protein expression in hypocotyls and stems of variety Suinong 10 carrying resistance genes Rps1a and Rps2 was investigated by using mRNA differential display reverse transcription PCR and two-dimensional electrophoresis at 0, 0.5, 1, 2, and 4 h after inoculation with P. sojae race 1. The results of the comparison of gene and protein expression showed that at least eight differential fragments at the transcriptional level were related to metabolic pathway, phytoalexin, and signal transduction in defense responses.Sequence analyses indicated that these fragments represented cinnamic acid 4-hydroxylase gene, ATP β gene coding ATP synthase β subunit and ubiquitin-conjugating enzyme gene which upregulated at 0.5 h post inoculation, blue copper protein gene and UDP-N-acetyl-α-D-galactosamine gene which upregulated at 2 h post inoculation, TGA-type basic leucine zipper protein TGA1.1 gene, cyclophilin gene, and 14-3-3 protein gene which upregulated at 4 h post inoculation.Three resistance-related proteins, α-subunit and β-subunit of ATP synthase, and cytochrome P450-like protein, were upregulated at 2 h post inoculation. The results suggested that resistance-related multiple proteins and genes were expressed in the recognition between soybean and P. sojae during zoospore germination, penetration and mycelium growth of P. sojae in soybean.

关键词: Phytophthora sojae, resistance mechanism, incompatible interaction, mRNA differential display reverse transcription PCR, two-dimensional electrophoresis

Abstract: Soybean root and stem rot caused by Phytophthora sojae is a destructive disease worldwide. Using genetic resistance is an important and major component in the integrated pest management of this disease. To understand molecular mechanisms of root and stem rot resistance in soybeans, the gene and protein expression in hypocotyls and stems of variety Suinong 10 carrying resistance genes Rps1a and Rps2 was investigated by using mRNA differential display reverse transcription PCR and two-dimensional electrophoresis at 0, 0.5, 1, 2, and 4 h after inoculation with P. sojae race 1. The results of the comparison of gene and protein expression showed that at least eight differential fragments at the transcriptional level were related to metabolic pathway, phytoalexin, and signal transduction in defense responses.Sequence analyses indicated that these fragments represented cinnamic acid 4-hydroxylase gene, ATP β gene coding ATP synthase β subunit and ubiquitin-conjugating enzyme gene which upregulated at 0.5 h post inoculation, blue copper protein gene and UDP-N-acetyl-α-D-galactosamine gene which upregulated at 2 h post inoculation, TGA-type basic leucine zipper protein TGA1.1 gene, cyclophilin gene, and 14-3-3 protein gene which upregulated at 4 h post inoculation.Three resistance-related proteins, α-subunit and β-subunit of ATP synthase, and cytochrome P450-like protein, were upregulated at 2 h post inoculation. The results suggested that resistance-related multiple proteins and genes were expressed in the recognition between soybean and P. sojae during zoospore germination, penetration and mycelium growth of P. sojae in soybean.

Key words: Phytophthora sojae, resistance mechanism, incompatible interaction, mRNA differential display reverse transcription PCR, two-dimensional electrophoresis