Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (6): 893-901.DOI: 10.1016/S1671-2927(11)60074-6

• 论文 • 上一篇    下一篇

Isolation and Expression Patterns of Rice WRKY82 Transcription Factor Gene Responsive to Both Biotic and Abiotic Stresses

PENG Xi-xu, TANG Xin-ke, ZHOU Ping-lan, HU Yao-jun, DENG Xiao-bo, HE Yan and WANG Hai-hua   

  1. School of Life Sciences, Hunan University of Science and Technology, Xiangtan 411201, P.R.China
  • 收稿日期:2011-06-10 修回日期:2011-06-10 出版日期:2011-06-20 发布日期:2011-06-03
  • 通讯作者: WAHNG Hai-hua
  • 作者简介:PENG Xi-xu, MSc, Tel: +86-731-58291416, E-mail: pengxix@163.com; Correspondence WAHNG Hai-hua, Professor, Ph D, Tel: +86-731-58290476, Fax: +86-731- 58290478, E-mail: haihuawxt@163.com

Isolation and Expression Patterns of Rice WRKY82 Transcription Factor Gene Responsive to Both Biotic and Abiotic Stresses

PENG Xi-xu, TANG Xin-ke, ZHOU Ping-lan, HU Yao-jun, DENG Xiao-bo, HE Yan and WANG Hai-hua   

  1. School of Life Sciences, Hunan University of Science and Technology, Xiangtan 411201, P.R.China
  • Received:2011-06-10 Revised:2011-06-10 Online:2011-06-20 Published:2011-06-03
  • Contact: WAHNG Hai-hua
  • About author:PENG Xi-xu, MSc, Tel: +86-731-58291416, E-mail: pengxix@163.com; Correspondence WAHNG Hai-hua, Professor, Ph D, Tel: +86-731-58290476, Fax: +86-731- 58290478, E-mail: haihuawxt@163.com

摘要: WRKY transcription factors are involved in the regulation of response to biotic and abiotic stresses in plants. A fulllength cDNA clone of rice WRKY82 gene (OsWRKY82) was isolated from a cDNA library generated from leaves infected by Magnaporthe grisea. OsWRKY82 contained an entire open reading frame in length of 1 701 bp, and was predicted to encode a polypeptide of 566 amino acid residues consisting of two WRKY domains, each with a zinc finger motif of C2H2,belonging to the WRKY subgroup I. OsWRKY82 shared high identity at the amino acid level with those from Sorghum bicolor, Hordeum vulgare, and Zea mays. The transcript level of OsWRKY82 was relatively higher in stems, leaves, and flowers, and less abundant in grains. It was induced by inoculation with M. grisea and Rhizoctonia solani. However, the inducible expression in incompatible rice-M. grisea interactions was earlier and greater than that in compatible interactions.The expression of OsWRKY82 was up-regulated by methyl jasmonate and ethephon, whereas salicylic acid exerted no effects on its expression. Moreover, OsWRKY82 exhibited transcriptional activation ability in yeast. Additionally,OsWRKY82 transcripts could be induced by wounding and heat shocking, but not by abscisic acid, cold, high salinity and dehydration. By contrast, gibberellin suppressed the expression of OsWRKY82. These indicate that OsWRKY82 is a multiply stress-inducible gene responding to both biotic and abiotic stresses, and may be involved in the regulation of defense response to pathogens and tolerance against abiotic stresses by jasmonic acid/ethylene-dependent signaling pathway.

关键词: WRKY transcription factor, biotic stress, abiotic stress, gene expression, Oryza sativa

Abstract: WRKY transcription factors are involved in the regulation of response to biotic and abiotic stresses in plants. A fulllength cDNA clone of rice WRKY82 gene (OsWRKY82) was isolated from a cDNA library generated from leaves infected by Magnaporthe grisea. OsWRKY82 contained an entire open reading frame in length of 1 701 bp, and was predicted to encode a polypeptide of 566 amino acid residues consisting of two WRKY domains, each with a zinc finger motif of C2H2,belonging to the WRKY subgroup I. OsWRKY82 shared high identity at the amino acid level with those from Sorghum bicolor, Hordeum vulgare, and Zea mays. The transcript level of OsWRKY82 was relatively higher in stems, leaves, and flowers, and less abundant in grains. It was induced by inoculation with M. grisea and Rhizoctonia solani. However, the inducible expression in incompatible rice-M. grisea interactions was earlier and greater than that in compatible interactions.The expression of OsWRKY82 was up-regulated by methyl jasmonate and ethephon, whereas salicylic acid exerted no effects on its expression. Moreover, OsWRKY82 exhibited transcriptional activation ability in yeast. Additionally,OsWRKY82 transcripts could be induced by wounding and heat shocking, but not by abscisic acid, cold, high salinity and dehydration. By contrast, gibberellin suppressed the expression of OsWRKY82. These indicate that OsWRKY82 is a multiply stress-inducible gene responding to both biotic and abiotic stresses, and may be involved in the regulation of defense response to pathogens and tolerance against abiotic stresses by jasmonic acid/ethylene-dependent signaling pathway.

Key words: WRKY transcription factor, biotic stress, abiotic stress, gene expression, Oryza sativa