非洲栽培稻基因 OgGS3.2在亚洲栽培稻中正调控粒型

doi:10.1016/j.jia.2026.06.019

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (10): 3941-3950.DOI: 10.1016/j.jia.2026.06.019

非洲栽培稻基因 OgGS3.2在亚洲栽培稻中正调控粒型

收稿日期:2026-03-04 修回日期:2026-06-11 接受日期:2026-05-19 出版日期:2026-10-20 发布日期:2026-09-21

The African rice gene OgGS3.2 enhances grain size in Asian rice

Wei He, Linhua Wu, Bin Gao, Wenkai Luo, Jing Ning, Leqin Chang, Min Hu, Liang Luo, Wenguang Wu^#, Zuofeng Zhu^#

Frontiers Science Center for Molecular Design Breeding, Ministry of Education/State Key Laboratory of Maize Bio-breeding/National Center for Evaluation of Agricultural Wild Plants (Rice)/Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, China

Received:2026-03-04 Revised:2026-06-11 Accepted:2026-05-19 Online:2026-10-20 Published:2026-09-21
About author:#Correspondence Zuofeng Zhu, E-mail: zhuzf@cau.edu.cn; Wenguang Wu, E-mail: wuwenguang@cau.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (32261143756 and 31925029) and the Chinese Universities Scientific Fund (2024TC195).

摘要/Abstract

摘要：

将近缘物种的基因组片段导入作物是丰富遗传多样性、创制新种质的有效途径。非洲栽培稻（Oryza glaberrima）作为两个栽培稻种之一，是改良亚洲栽培稻（O. sativa）的宝贵遗传资源。粒型是决定水稻产量的关键因素。本研究鉴定了一个携带非洲栽培稻单一染色体片段的渗入系。与轮回亲本相比，该渗入系的籽粒显著增大，而其他农艺性状未受明显影响。通过图位克隆，从该片段上鉴定到粒型基因 OgGS3.2，其是 OsSLR1的同源基因。进一步分析表明，OgGS3.2中的一个单核苷酸替换为导致籽粒增大的关键变异。单倍型分析显示，绝大多数非洲栽培稻种质在 GS3.2位点携带 G 类单倍型。本研究揭示了 OgGS3.2调控粒型的分子机制，并为提高栽培稻产量提供了策略性遗传资源。

Abstract:

Introducing genomic segments from related species into crops is an effective approach to enrich genetic diversity and create novel germplasm. African cultivated rice (Oryza glaberrima), one of the two cultivated rice species, represents a valuable genetic resource for improving Asian cultivated rice (O. sativa). Grain size/weight is among the most important components of grain yield in rice. In this study, we isolated a chromosome segment introgression line harboring a single segment derived from O. glaberrima. Compared to the recurrent parent, this line displayed a significant increase in grain size, whereas other agronomic traits were not significantly affected. Using map-based cloning, we identified OgGS3.2 from this fragment as an ortholog of OsSLR1. A single nucleotide substitution in OgGS3.2 was identified as the causal mutation underlying the increased seed size phenotype. Haplotype analysis revealed that the vast majority of African rice germplasm carries the G haplotype at the GS3.2 locus. This study reveals the molecular mechanism by which OgGS3.2 regulates grain size and offers strategic genetic resources for yield improvement in cultivated rice.

Key words: Oryza glaberrima , Oryza sativa L. , grain size , chromosome segment introgression lines

Wei He, Linhua Wu, Bin Gao, Wenkai Luo, Jing Ning, Leqin Chang, Min Hu, Liang Luo, Wenguang Wu, Zuofeng Zhu. 非洲栽培稻基因 OgGS3.2在亚洲栽培稻中正调控粒型[J]. Journal of Integrative Agriculture, 2026, 25(10): 3941-3950.

Wei He, Linhua Wu, Bin Gao, Wenkai Luo, Jing Ning, Leqin Chang, Min Hu, Liang Luo, Wenguang Wu, Zuofeng Zhu. The African rice gene OgGS3.2 enhances grain size in Asian rice[J]. Journal of Integrative Agriculture, 2026, 25(10): 3941-3950.

参考文献

Agarwal M, Shrivastava N, Padh H. 2008. Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Reports, 27, 617–631.

Ali M L, Sanchez P L, Yu S B, Lorieux M, Eizenga G C. 2010. Chromosome segment substitution lines: A powerful tool for the introgression of valuable genes from Oryza wild species into cultivated rice (O. sativa). Rice, 3, 218–234.

Badawi A T. 2004. Rice-based production systems for food security and poverty alleviation in the Near East and North Africa. International Rice Commission Newsletter, 53, 102–114.

Balakrishnan D, Surapaneni M, Mesapogu S, Neelamraju S. 2018. Development and use of chromosome segment substitution lines as a genetic resource for crop improvement. Theoretical and Applied Genetics, 132, 1–25.

Bart R, Chern M, Park C J, Bartley L, Ronald P C. 2006. A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts. Plant Methods, 2, 13.

Chen E, Hou Q, Liu K, Gu Z, Dai B, Wang A, Feng Q, Zhao Y, Zhou C, Zhu J, Shangguan Y, Wang Y, Lv D, Fan D, Huang T, Wang Z, Huang X, Han B. 2023. Armadillo repeat only protein GS10 negatively regulates brassinosteroid signaling to control rice grain size. Plant Physiology, 192, 967–981.

Fernandes T, Gonçalves N M, Matiolli C C, Rodrigues M A A, Barros P M, Oliveira M M, Abreu I A. 2024. SUMOylation of rice DELLA SLR1 modulates transcriptional responses and improves yield under salt stress. Planta, 260, 136.

Fukuta Y, Konisho K, Senoo-Namai S, Yanagihara S, Tsunematsu H, Fukuo A, Kumashiro T. 2012. Genetic characterization of rainfed upland New Rice for Africa (NERICA) varieties. Breeding Science, 62, 27–37.

Hu J, Wang Y, Fang Y, Zeng L, Xu J, Yu H, Shi Z, Pan J, Zhang D, Kang S, Zhu L, Dong G, Guo L, Zeng D, Zhang G, Xie L, Xiong G, Li J, Qian Q. 2015. A rare allele of GS2 enhances grain size and grain yield in rice. Molecular Plant, 8, 1455–1465.

Huang D, Wang S, Zhang B, Shang-Guan K, Shi Y, Zhang D, Liu X, Wu K, Xu Z, Fu X, Zhou Y. 2015. A gibberellin-mediated DELLA-NAC signaling cascade regulates cellulose synthesis in rice. The Plant Cell, 27, 1681–1696.

Ikeda A, Kitano H, Sonoda Y, Futsuhara Y, Yamaguchi J. 2008. Characterizing a slender mutant with constitutive gibberellin-response in rice. In: Advances in Rice Genetics. World Scientific Publishing Company, Los Baños. pp. 480–482.

Ikeda A, Ueguchi-Tanaka M, Sonoda Y, Kitano H, Koshioka M, Futsuhara Y, Matsuoka M, Yamaguchi J. 2001. The slender rice mutant, with constitutively activated gibberellin signal transduction, has enhanced capacity for abscisic acid level. The Plant Cell, 13, 999–1010.

Jin Y, Song X, Chang H, Zhao Y, Cao C, Qiu X, Zhu J, Wang E, Yang Z, Yu N. 2022. The GA-DELLA-OsMS188 module controls male reproductive development in rice. New Phytologist, 233, 2629–2642.

Kijima Y, Sserunkuuma D, Otsuka K. 2006. How revolutionary is the “NERICA revolution”? Evidence from Uganda. The Developing Economies, 44, 252–267.

Kovach M J, Sweeney M T, McCouch S R. 2007. New insights into the history of rice domestication. Trends in Genetics, 23, 578–587.

Lan J, Lin Q, Zhou C, Ren Y, Liu X, Miao R, Jing R, Mou C, Nguyen T, Zhu X, Wang Q, Zhang X, Guo X, Liu S, Jiang L, Wan J. 2020. Small grain and semi-dwarf 3, a WRKY transcription factor, negatively regulates plant height and grain size by stabilizing SLR1 expression in rice. Plant Molecular Biology, 104, 429–450.

Li S, Tian Y, Wu K, Ye Y, Yu J, Zhang J, Liu Q, Hu M, Li H, Tong Y, Harberd N P, Fu X. 2018. Modulating plant growth-metabolism coordination for sustainable agriculture. Nature, 560, 595–600.

Li X M, Chao D Y, Wu Y, Huang X, Chen K, Cui L G, Su L, Ye W W, Chen H, Chen H C, Dong N Q, Guo T, Shi M, Feng Q, Zhang P, Han B, Shan J X, Gao J P, Lin H X. 2015. Natural alleles of a proteasome α2 subunit gene contribute to thermotolerance and adaptation of African rice. Nature Genetics, 47, 827–833.

Li Y, Huang K, Zhang L, Zhang B, Duan P, Zhang G, Huang X, Zhou C, Han N, Zheng L, Wang Y, Li Y. 2025. A molecular framework for the GS2-SUG1 module-mediated control of grain size and weight in rice. Nature Communications, 16, 3944.

Liao Z, Yu H, Duan J, Yuan K, Yu C, Meng X, Kou L, Chen M, Jing Y, Liu G, Smith S M, Li J. 2019. SLR1 inhibits MOC1 degradation to coordinate tiller number and plant height in rice. Nature Communications, 10, 2738.

Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X, Dong Z, Liu Y G. 2015. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Molecular Plant, 8, 1274–1284.

Mao H, Sun S, Yao J, Wang C, Yu S, Xu C, Li X, Zhang Q. 2010. Linking differential domain functions of the GS3 protein to natural variation of grain size in rice. Proceedings of the National Academy of Sciences of the United States of America, 107, 19579–19584.

Meyer R S, Choi J Y, Sanches M, Plessis A, Flowers J M, Amas J, Dorph K, Barretto A, Gross B, Fuller D Q, Bimpong I K, Ndjiondjop M N, Hazzouri K M, Gregorio G B, Purugganan M D. 2016. Domestication history and geographical adaptation inferred from a SNP map of African rice. Nature Genetics, 48, 1083–1088.

Sarfaraz H. 2023. Rice production, quality, and nutrition: A comprehensive review on challenges, opportunities, and global perspectives. International Journal of Agriculture & Sustainable Development, 5, 90–103.

Sun T P. 2010. Gibberellin-GID1-DELLA: A pivotal regulatory module for plant growth and development. Plant Physiology, 154, 567–570.

Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow T Y, Hsing Y I C, Kitano H, Yamaguchi I, Matsuoka M. 2005. GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature, 437, 693–698.

Wang W, Mauleon R, Hu Z, Chebotarov D, Tai S, Wu Z, Li M, Zheng T, Fuentes R R, Zhang F, Mansueto L, Copetti D, Sanciangco M, Palis K C, Xu J, Sun C, Fu B, Zhang H, Gao Y, Zhao X, et al. 2018. Genomic variation in 3,010 diverse accessions of Asian cultivated rice. Nature, 557, 43–49.

Wu W, Liu X, Wang M, Meyer R S, Luo X, Ndjiondjop M N, Tan L, Zhang J, Wu J, Cai H, Sun C, Wang X, Wing R A, Zhu Z. 2017. A single-nucleotide polymorphism causes smaller grain size and loss of seed shattering during African rice domestication. Nature Plants, 3, 17064.

Xing Y, Zhang Q. 2010. Genetic and molecular bases of rice yield. Annual Review of Plant Biology, 61, 421–442.

Zhang B, Ma L, Wu B, Xing Y, Qiu X. 2022. Introgression lines: Valuable resources for functional genomics research and breeding in rice (Oryza sativa L.). Frontiers in Plant Science, 13, 863789.

Zhang S, Deng L, Cheng R, Hu J, Wu C Y. 2022. RID1 sets rice heading date by balancing its binding with SLR1 and SDG722. Journal of Integrative Plant Biology, 64, 149–165.

Zhang Y M, Yu H X, Ye W W, Shan J X, Dong N Q, Guo T, Kan Y, Xiang Y H, Zhang H, Yang Y B, Li Y C, Zhao H Y, Lu Z Q, Guo S Q, Lei J J, Liao B, Mu X R, Cao Y J, Yu J J, Lin H X. 2021. A rice QTL GS3.1 regulates grain size through metabolic-flux distribution between flavonoid and lignin metabolons without affecting stress tolerance. Communications Biology, 4, 1171.

非洲栽培稻基因 OgGS3.2在亚洲栽培稻中正调控粒型

The African rice gene OgGS3.2 enhances grain size in Asian rice

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