Detection and characterization of Hepatitis E virus from commercial rabbit livers in Hebei, China

doi:10.1016/S2095-3119(21)63632-8

Journal of Integrative Agriculture ›› 2021, Vol. 20 ›› Issue (12): 3270-3276.DOI: 10.1016/S2095-3119(21)63632-8

收稿日期:2020-10-09 出版日期:2021-12-01 发布日期:2021-10-20

Detection and characterization of Hepatitis E virus from commercial rabbit livers in Hebei, China

XIAO Peng^1*, TIAN Ji-jing^1*, MAO Jing-jing^{1, 2}, GUO Zhao-jie¹, ZHAO Yue³, LIU Tian-long¹, CHEN Jian¹, WANG Tong-tong¹, MA Long-huan¹, SHE Rui-ping¹

¹ Laboratory of Animal Pathology and Public Health/Key Laboratory of Zoonosis of Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China
² Shanghai InnoStar Bio-Tech Co., Shanghai 201203, P.R.China
³ Institute of Laboratory Animal Science, Jinan University, Guangzhou 510632, P.R.China

Received:2020-10-09 Online:2021-12-01 Published:2021-10-20
Contact: Correspondence SHE Rui-ping, E-mail: sheruiping@126.com
About author:* These authors contributed equally to this study.
Supported by:
This study was supported by the National Natural Science Foundation of China (31802162, 31472165 and 31272515). The funders had no role in study design, data collection, and interpretation, or the decision to submit the work for publication.

摘要/Abstract

摘要：

据报道，兔戊型肝炎病毒 (HEV) 为潜在人畜共患性传染病。报道显示HEV 3型是造成兔HEV流行的主要原因。为调查戊型肝炎在肉兔中的流行情况，本研究从中国河北省的一个屠宰场收集了176份肝脏样本。RNA检测结果显示3份样本HEV-ORF2检测阳性，占样本量的1.7%。序列分析表明，3株分离株具有较高的同源性 (94.08%~98.85%)，为HEV基因3型。此外，免疫组化 (IHC) 检测HEV ORF2蛋白显示，131份肝脏样本阳性，占样本量的74.4%。肝脏组织病理学观察结果显示，H.E染色的组织病理学变化主要有肝细胞变性、坏死、淋巴细胞浸润、胆管上皮细胞增生、含铁血黄素沉着等。这些结果表明，在家兔中存在戊型肝炎病毒，但兔戊肝病毒 (rHEV) 的传染性及人兔跨种传播的潜在风险有待进一步研究。

Abstract:

Rabbit hepatitis E virus (HEV) has been reported for years and is thought to have the potential for zoonotic transmission from rabbits to humans. As reported, HEV genotype 3 (gt3) is the most prevalent form of HEV in rabbits. To determine the prevalence of HEV in commercial rabbit livers, 176 liver samples were collected from an abattoir in Hebei Province, China. Three (1.7%) samples tested positive for RNA of HEV-ORF2 (open reading frames-2). Sequence analysis showed that the three isolates shared high identities with each other (94.08–98.85%). Further analysis showed that all the rabbit strains clustered together in the branch of HEV gt3. Further study by immunohistochemistry (IHC) assays showed that 131 (74.4%) liver samples were positive for HEV ORF2 protein. Pathological changes including cell degeneration, inflammatory cell infiltration and bile duct epithelial cell hyperplasia were observed under microscopy. These findings indicated the presence of HEV in commercial livers of rabbits. Additional studies should be conducted to investigate the infectivity of rabbit HEV (rHEV) and the potential risks of zoonotic transmission of rHEV from rabbits to human beings.

Key words: hepatitis E virus , IHC , RT-nPCR , commercial rabbits , livers

. [J]. Journal of Integrative Agriculture, 2021, 20(12): 3270-3276.

XIAO Peng, TIAN Ji-jing, MAO Jing-jing, GUO Zhao-jie, ZHAO Yue, LIU Tian-long, CHEN Jian, WANG Tong-tong, MA Long-huan, SHE Rui-ping. Detection and characterization of Hepatitis E virus from commercial rabbit livers in Hebei, China[J]. Journal of Integrative Agriculture, 2021, 20(12): 3270-3276.

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Detection and characterization of Hepatitis E virus from commercial rabbit livers in Hebei, China

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