Deciphering the morpho–physiological traits for high yield potential in nitrogen efficient varieties (NEVs): A japonica rice case study

doi:10.1016/S2095-3119(20)63600-0

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (4): 947-963.DOI: 10.1016/S2095-3119(20)63600-0

所属专题：水稻耕作栽培合辑Rice Physiology · Biochemistry · Cultivation · Tillage

收稿日期:2020-09-18 接受日期:2020-01-09 出版日期:2022-04-01 发布日期:2021-01-09

Deciphering the morpho–physiological traits for high yield potential in nitrogen efficient varieties (NEVs): A japonica rice case study

ZHU Kuan-yu¹, YAN Jia-qian¹, SHEN Yong¹, ZHANG Wei-yang¹, XU Yun-ji², WANG Zhi-qin¹, YANG Jian-chang¹

¹ Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, P.R.China
² Joint International Research Laboratory of Agriculture and Agri-product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, P.R.China

Received:2020-09-18 Accepted:2020-01-09 Online:2022-04-01 Published:2021-01-09
About author:ZHU Kuan-yu, Tel/Fax: +86-514-87979317, E-mail: 1659855942@qq.com; Correspondence YANG Jian-chang, Tel/Fax: +86-514-87979317, E-mail: jcyang@yzu.edu.cn
Supported by:
This work was supported by the grants from the National Natural Science Foundation of China (32071843, 31901444 and 31901445), the National Key Research and Development Program of China (2016YFD0300206-4 and 2018YFD0300800), the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD), the Top Talent Supporting Program of Yangzhou University, China (2015-01), the Natural Science Foundation of Jiangsu Province, China (BK20190880), and the Natural Science Foundation of Jiangsu Higher Education Institutions, China (19KJB210019).

摘要/Abstract

摘要：

选用氮高效水稻品种可减少氮肥的投入而不降低产量，但有关氮高效水稻品种的形态生理特征不清楚。本研究以3个氮高效粳稻品种和3个氮低效粳稻品种为材料并种植于大田，设置不施氮(0 kg N ha^-1)和常规施氮量(180或200 kg N ha^-1)2种处理。结果表明，与氮低效品种相比，氮高效品种在2种施氮量情况下均具有较高的总颖花量、结实率、产量和氮肥利用率。氮高效品种在穗分化期具有较高的根系氧化力、根干重、根长和根直径，灌浆期具有较高的粒叶比和茎蘖成穗率，抽穗后具有较高的氮转运量、干物质积累、良好的叶片性状包括较高的剑叶厚度、比叶重、光合速率，较高的核酮糖-1,5-二磷酸羧化酶/加氧酶、叶绿素、氮和可溶性糖含量，以及较优的冠层结构（较高的氮消减系数与消光系数之比）。上述形态生理性状与产量及氮肥利用率呈显著或极显著正相关。这些性状可作为培育和筛选氮高效水稻品种的指标。

Abstract: The use of nitrogen (N)-efficient rice (Oryza sativa L.) varieties could reduce excessive N input without sacrificing yields. However, the plant traits associated with N-efficient rice varieties have not been fully defined or comprehensively explored. Here, three japonica N-efficient varieties (NEVs) and three japonica N-inefficient varieties (NIVs) of rice were grown in a paddy field under N omission (0 N, 0 kg N ha^–1) and normal N (NN, 180 or 200 kg N ha⁻¹) treatments. Results showed that NEVs exhibited higher grain yield and nitrogen use efficiency (NUE) than NIVs under both treatments, due to improved sink size and filled-grains percentage in the former which had higher root oxidation activity and greater root dry weight, root length and root diameter at panicle initiation (PI), as well as higher spikelet–leaf ratio and more productive tillers during the grain-filling stage. Compared with NIVs, NEVs also exhibited enhanced N translocation and dry matter accumulation after heading and improved flag leaf morpho–physiological traits, including greater leaf thickness and specific leaf weight and higher contents of ribulose-1,5-bisphosphate carboxylase/oxygenase, chlorophyll, nitrogen, and soluble sugars, leading to better photosynthetic performance. Additionally, NEVs had a better canopy structure, as reflected by a higher ratio of the extinction coefficient for effective leaf N to the light extinction coefficient, leading to enhanced canopy photosynthesis and dry matter accumulation. These improved agronomic and physiological traits were positively and significantly correlated with grain yield and internal NUE, which could be used to select and breed N-efficient rice varieties.

Key words: rice , N-efficient varieties , nitrogen use efficiency , root morpho–physiological traits , nitrogen translocation , leaf morphology , canopy structure

. [J]. Journal of Integrative Agriculture, 2022, 21(4): 947-963.

ZHU Kuan-yu, YAN Jia-qian, SHEN Yong, ZHANG Wei-yang, XU Yun-ji, WANG Zhi-qin, YANG Jian-chang. Deciphering the morpho–physiological traits for high yield potential in nitrogen efficient varieties (NEVs): A japonica rice case study[J]. Journal of Integrative Agriculture, 2022, 21(4): 947-963.

参考文献

Anten N, Schieving F, Werger M. 1995. Patterns of light and nitrogen distribution in relation to whole canopy carbon gain in C3 and C4 mono- and di-cotyledonous species. Oecologia, 101, 504–513.
Arnon D I. 1949. Copper enzymes in isolated chloroplasts; polyphenoloxidase in Beta vulgaris L. Plant Physiology, 24, 1–15.
Buckley T N, Cescatti A, Farquhar G D. 2013. What does optimization theory actually predict about crown profiles of photosynthetic capacity when models incorporate greater realism? Plant, Cell and Environment, 36, 1547–1563.
Chen C, Yang B, Zhu Z K, Cao W Y, Luo G, Zhou J, Wang X J, Yu X F, Yuan Q M, Zhong J, Huang Y, Huang J Y, Wang Y L, Dong G C. 2015. Root traits affecting nitrogen efficient absorption in rice genetic populations. Chinese Journal of Rice Science, 29, 390–398. (in Chinese)
Chen F, Fang Z, Gao Q, Ye Y, Jia L, Yuan L, Mi G, Zhang F. 2013. Evaluation of the yield and nitrogen use efficiency of the dominant maize hybrids grown in North and Northeast China. Science China (Life Sciences), 56, 552–560.
Chu G, Chen S, Xu C, Wang D, Zhang X. 2019. Agronomic and physiological performance of indica/japonica hybrid rice cultivar under low nitrogen conditions. Field Crops Research, 243, 107625.
Chu G, Chen T, Chen S, Xu C, Wang D, Zhang X. 2018. Agronomic performance of drought-resistance rice cultivars grown under alternate wetting and drying irrigation management in southeast China. The Crop Journal, 6, 482–494.
Dreccer M, Oijen M, Schapendonk A, Pot C, Rabbinge R. 2000. Dynamics of vertical leaf nitrogen distribution in a vegetative wheat canopy. Impact on canopy photosynthesis. Annals of Botany, 86, 821–831.
Fan M S, Shen J B, Yuan L X, Jiang R F, Chen X P, Davies W J, Zhang F S. 2012. Improving crop productivity and resource use efficiency to ensure food security and environmental quality in China. Journal of Experimental Botany, 63, 13–24.
Foulkes M J, Hawkesford M J, Barraclough P B, Holdsworth M J, Kerr S, Kightley S, Shewry P R. 2009. Identifying traits to improve the nitrogen economy of wheat: Recent advances and future prospects. Field Crops Research, 114, 329–342.
Gallagher J N, Biscoe P V. 1978. Radiation absorption, growth and yield of cereals. Journal of Agricultural and Food Chemistry, 91, 47–60.
Gallais A, Coque M. 2005. Genetic variation and selection for nitrogen use efficiency in maize: A synthesis. Maydica, 50, 531–547.
Gastal F, Lemaire G. 2002. N uptake and distribution in crops: An agronomical and eco-physiological perspective. Journal of Experimental Botany, 53, 789–799.
Gu J F, Chen Y, Zhang H, Li Z K, Zhou Q, Yu C, Kong X S, Liu L J, Wang Z Q, Yang J C. 2017. Canopy light and nitrogen distributions are related to grain yield and nitrogen use efficiency in rice. Field Crops Research, 206, 74–85.
Hedden P. 2003. The genes of the Green Revolution. Trends in Genetics, 19, 5–9.
Hikosaka K. 2014. Optimal nitrogen distribution within a leaf canopy under direct and diffuse light. Plant, Cell and Environment, 37, 2077–2085.
Huang L, Yang D, Li X, Peng S, Wang F. 2019. Coordination of high grain yield and high nitrogen use efficiency through large sink size and high post-heading source capacity in rice. Field Crops Research, 233, 49–58.
Ju C X. 2017. Agronomic and physiological traits for rice cultivars differing in response to nitrogen. Ph D thesis, Yangzhou University, Yangzhou, Jiangsu, China. (in Chinese)
Ju C X, Buresh R J, Wang Z Q, Zhang H, Liu L J, Yang J C, Zhang J H. 2015. Root and shoot traits for rice varieties with higher grain yield and higher nitrogen use efficiency at lower nitrogen rates application. Field Crops Research, 175, 47–55.
Ju X T, Xing G X, Chen X P, Zhang S L, Zhang L J, Liu X J, Cui Z L, Yin B, Christiea P, Zhu Z L, Zhang F S. 2009. Reducing environmental risk by improving N management in intensive Chinese agricultural systems. Proceedings of the National Academy of Sciences of the United States of America, 106, 3041–3046.
Kato T, Takeda K. 1996. Associations among characters related to yield sink capacity in space-planted rice. Crop Science, 36, 1135–1139.
Lawlor D W. 2002. Carbon and nitrogen assimilation in relation to yield: Mechanisms are the key to understanding production systems. Journal of Experimental Botany, 53, 773–787.
Li G H, Xue L H, Gu W, Yang C D, Wang S H, Ling Q H, Qin X, Ding Y F. 2009. Comparison of yield components and plant type characteristics of high-yield rice between Taoyuan, a ‘special eco-site’ and Nanjing, China. Field Crops Research, 112, 214–221.
Li H W, Liu L J, Wang Z Q, Yang J C, Zhang J H. 2012. Agronomic and physiological performance of high-yielding wheat and rice in the lower reaches of Yangtze River of China. Field Crops Research, 133, 119–129.
Ling Q H, Yang J C. 1986. Studies on “grain–leaf ratio” of population and cultural approaches of high yield in rice plants. Scientia Agricutura Sinica, 19, 1–8. (in Chinese)
Ma L H, Zhang Y, Sui b, Liu C L, Wang P, Gu S D, Shen Q R, Xu M, Guo S W. 2011. The impact factors of excessive fertilization in Jiangsu Province. Journal of Yangzhou Univerity (Agricultural and Life Science Edition), 32, 48–52, 80. (in Chinese)
Makino A, Mae T, Ohira K. 1985. Enzymic properties of ribulose-1,5-bisphosphate carboxylase/oxygenase purified from rice leaves. Plant Physiology, 79, 57–61.
Mishima S I, Taniguchi S, Komada M. 2006. Recent trends in nitrogen and phosphate use and balance on Japanese farmland. Soil Science of Plant Nutrition, 52, 556–563.
Ort D R, Zhu X, Melis A. 2011. Optimizing antenna size to maximize photosynthetic efficiency. Plant Physiology, 155, 79–85.
Peng S, Khush G S, Virk P, Tang Q, Zou Y. 2008. Progress in ideotype breeding to increase rice yield potential. Field Crops Research, 108, 32–38.
Peng S, Tang Q, Zou Y. 2009. Current status and challenges of rice production in China. Plant Production, 12, 3–8.
Peng Y, Li X, White P, Li C. 2015. A large and deep root system underlies high nitrogen-use efficiency in maize production. PLoS ONE, 10, e0126293.
Ramasamy S, Berge H, Purushothaman S. 1997. Yield formation in rice in response to drainage and nitrogen application. Field Crops Research, 51, 65–82.
Surya K, Bi Y Mei, Rothstein S J. 2011. Understanding plant response to nitrogen limitation for the improvement of crop nitrogen use efficiency. Journal of Experimental Botany, 62, 1499–1509.
Valluru R, Link J, Claupein W. 2011. Natural variation and morpho-physiological traits associated with water-soluble carbohydrate concentration in wheat under different nitrogen levels. Field Crops Research, 124, 104–113.
Vile D, Garnier E, Shipley B, Laurent G, Navas M, Roumet C, Lavorel S, Díaz S, Hodgson J G, Lloret F, Midgley G, Poorter H, Rutherford M, Wilson P J, Wright I. 2005. Specific leaf area and dry matter content estimate thickness in laminar leaves. Annals of Botany, 96, 1129–1136.
Wei H H, Yang Y L, Shao X Y, Shi T Y, Dai Q G. 2020. Higher leaf area through leaf width and lower leaf angle were the primary morphological traits for yield advantage of japonica/indica hybrids. Journal of Integrative Agriculture, 19, 483–494.
Wu H. 2018. Study on characteristics of post-anthesis matter production and translocation of carbon and nitrogen in indica and japonica hybrid rice. Ph D thesis, Huazhong Agricultural University, Wuhan, Hubei, China. (in Chinese)
Wu H, Xiang J, Zhang Y, Zhang Y, Peng S, Chen H, Zhu D. 2018. Effects of post-anthesis nitrogen uptake and translocation on photosynthetic production and rice yield. Scientific Reports, 8, 12891.
Wu L, Yuan S, Huang L, Sun F, Zhu G, Li G, Fahad S, Peng S, Wang F. 2016. Physiological mechanisms underlying the high-grain yield and high-nitrogen use efficiency of elite rice varieties under a low rate of nitrogen application in China. Frontiers in Plant Science, 7, 1024.
Wu Y, Liu W, Li X, Li M, Zhang D, Hao Z, Weng J, Xu Y, Bai L, Zhang S. 2011. Low-nitrogen stress tolerance and nitrogen agronomic efficiency among maize inbreds: Comparison of multiple indices and evaluation of genetic variation. Euphytica,
180, 281–290.
Xi T. 2019. QTL mapping of flag leaf length and width correlation genes in rice and preliminary construction of introgression lines of rice. MSc thesis, Huazhong Agricultural University, Wuhan, Hubei, China. (in Chinese)
Xiong D, Wang D, Liu X, Peng S, Huang J, Li Y. 2016. Leaf density explains variation in leaf mass per area in rice between cultivars and nitrogen treatments. Annals of Botany, 117, 963–971.
Xiong D, Yu T, Zhang T, Li Y, Peng S, Huang J. 2015. Leaf hydraulic conductance is coordinated with leaf morpho-anatomical traits and nitrogen status in the genus Oryza. Journal of Experimental Botany, 66, 741–748.
Xue Y G, Duan H, Liu L J, Wang Z Q, Yang J C, Zhang J H. 2013. An improved crop management increases grain yield and nitrogen and water use efficiency in rice. Crop Science, 53, 271–284.
Yang J C, Zhan M F, Zhu K Y. 2018. Physiological bases for the formation of green traits in rice. Chinese Bulletin of Life Sciences, 30, 1137–1145. (in Chinese)
Yang J C, Zhang J H. 2006. Grain filling of cereals under soil drying. New Physiologist, 169, 223–236.
Yang J C, Zhang J H, Liu L J, Wang Z Q, Zhu Q S. 2002. Carbon remobilization and grain filling in japonica/indica hybrid rice subjected to post-anthesis water deficits. Agronomy Journal, 94, 102–109.
Yang J C, Zhang J H, Wang Z Q, Liu L J, Zhu Q S. 2003. Post-anthesis water deficits enhance grain filling in two-line hybrid rice. Crop Science, 43, 2099–2108.
Yang J C, Zhang H, Zhang J H. 2012. Root morphology and physiology in relation to the yield formation of rice. Journal of Integrative Agriculture, 11, 920–926.
Yang J C, Zhu Q S, Cao X Z. 1992. Effects of the structure and photosynthetic characters of the canopy on the yield formation in rice plants. Scientia Agricutura Sinica, 25, 7–14. (in Chinese)
Yin X, van Laar H. 2005. Crop Systems Dynamics: An Ecophysiological Simulation Model for Genotype-by-Environment Interactions. Wagningen Academic Publishers, Wageningen, The Netherlands.
Yin X, Lantinga E A, Schapendonk A H C M, Zhong X. 2003. Some quantitative relationships between leaf area index and canopy nitrogen content and distribution. Annals of Botany, 91, 893–903.
Yoshida S, Forno D, Cock J, Gomez K. 1976. Laboratory Manual for Physiological Studies of Rice. International Rice Research Institute, The Philippines. pp. 24–79.
Zhang H, Xue Y G, Wang Z Q, Yang J C, Zhang J H. 2009. An alternate wetting and moderate soil drying regime improves root and shoot growth in rice. Crop Science, 49, 2246–2260.
Zhang H C, Zhang J, Gong J L, Chang Y, Li M, Gao H, Dai Q G, Huo Z Y, Xu K, Wei H Y. 2013. The productive advantages and formation mechanisms of “Indica Rice to Japonica Rice”. Scientia Agricultura Sinica, 46, 686–704. (in Chinese)
Zhang W Y. 2018. Mechanism underlying water and nitrogen regulating spikelet development and grain filling of rice. Ph D thesis, Yangzhou University, Yangzhou, Jiangsu, China. (in Chinese)
Zhang W Y, Zhu K Y, Wang Z Q, Zhang H, Gu J F, Liu L J, Yang J C, Zhang J H. 2019. Brassinosteroids function in spikelet differentiation and degeneration in rice. Journal of Integrative Plant Biology, 61, 943–963.
Zhang X C, Huang W X, Zhu K Y, Wang Z Q, Yang J C. 2018. Effects of nitrogen on the nitrogen use efficiency and agronomic traits of different rice cultivars. Crops, 4, 67–78. (in Chinese)
Zhao X, Zhou Y, Min J, Wang S Q, Shi W M, Xing G X. 2012. Nitrogen runoff dominates water nitrogen pollution from rice-wheat rotation in the Taihu Lake region of China. Agriculture, Ecosystems & Environment, 156, 1–11.
Zhu K Y, Zhou Q, Shen Y, Yan J Q, Xu Y J, Wang Z Q, Yang J C. 2020. Agronomic and physiological performance of an indica–japonica rice variety with a high yield and high nitrogen use efficiency. Crop Science, 60, 1556–1568.

Deciphering the morpho–physiological traits for high yield potential in nitrogen efficient varieties (NEVs): A japonica rice case study

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics