Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (8): 2056-2063.DOI: 10.1016/S2095-3119(19)62855-8

所属专题: 植物细菌真菌合辑Plant Bacteria/Fungus

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  • 收稿日期:2019-06-11 出版日期:2020-08-01 发布日期:2020-06-21

A LAMP-assay-based specific microbiota analysis reveals community dynamics and potential interactions of 13 major soybean root pathogens

YE Wen-wu1, 2, ZENG Dan-dan1, 2, XU Miao1, 2, YANG Jin1, 2, MA Jia-xin1, 2, WANG Yuan-chao1, 2, ZHENG Xiao-bo1, 2
  

  1. 1 Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P.R.China
    2 Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, P.R.China
  • Received:2019-06-11 Online:2020-08-01 Published:2020-06-21
  • Contact: Correspondence ZHENG Xiao-bo, E-mail: xbzheng@njau.edu.cn
  • About author: YE Wen-wu, Tel: +86-25-84399621, E-mail: yeww@njau.edu.cn;
  • Supported by:
    This work was supported by the grants to Prof. Zheng Xiaobo and Prof. Wang Yuanchao from the National Key R&D Program of China (2018YFD0201000), the earmarked fund for China Agriculture Research System (CARS-004-PS14), and the National Natural Science Foundation of China (31721004), and by the grant to Associate Prof. Ye Wenwu from the National Natural Science Foundation of China (31772140).

Abstract:

Soybean root diseases are associated with numerous fungal and oomycete pathogens; however, the community dynamics and interactions of these pathogens are largely unknown.  We performed 13 loop-mediated isothermal amplification (LAMP) assays that targeted specific soybean root pathogens, and traditional isolation assays.  A total of 159 samples were collected from three locations in the Huang-Huai-Hai region of China at three soybean growth stages (30, 60, and 90 days after planting) in 2016.  In LAMP results, we found that pathogen communities differed slightly among locations, but changed dramatically between soybean growth stages.  Phytophthora sojae, Rhizoctonia solani, and Fusarium oxysporum were most frequently detected at the early stage, whereas Phomopsis longicolla, Fusarium equiseti, and Fusarium virguliforme were most common in the later stages.  Most samples (86%) contained two to six pathogen species.  Interestingly, the less detectable species tended to exist in the samples containing more detected species, and some pathogens preferentially co-occurred in diseased tissue, including P. sojaeR. solaniF. oxysporum and F. virguliformeCalonectria ilicicola, implying potential interactions during infection.  The LAMP detection results were confirmed by traditional isolation methods.  The isolated strains exhibited different virulence to soybean, further implying a beneficial interaction among some pathogens.
 

Key words: soybean root disease ,  fungal and oomycete plant pathogens ,  soil-borne and seed-borne pathogens ,  LAMP assay ,  complex infection