Identification of a novel emaravirus infecting lilac through next-generation sequencing

doi:10.1016/S2095-3119(19)62806-6

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (8): 2064-2071.DOI: 10.1016/S2095-3119(19)62806-6

所属专题：植物病理合辑Plant Protection—Plant Pathology；植物病毒合辑Plant Virus

收稿日期:2019-06-03 出版日期:2020-08-01 发布日期:2020-06-21

Identification of a novel emaravirus infecting lilac through next-generation sequencing

WANG Ya-qin^1*, SONG Yu1^*, CAO Meng-ji², CHENG Qi¹, WU Jian-xiang¹, HU Tao¹

¹ State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
² National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, P.R.China

Received:2019-06-03 Online:2020-08-01 Published:2020-06-21
Contact: Correspondence HU Tao, E-mail: taohu1989@zju.edu.cn
About author:* These authors contributed equally to this study.
Supported by:
This research was funded by the Laboratory Technology Research Program, Zhejiang University, China (SJS201809).

摘要/Abstract

Abstract:

Emaraviruses are multipartite negative-sense single-stranded viruses that usually infect flowering plants. Using unbiased next-generation sequencing (NGS) approach, a novel virus from lilac leaves with yellow mottle symptom was identified. Five viral genomic RNA segments were detected and completely sequenced. The molecular characteristics of the viral RNAs and proteins resemble those of members of the genus Emaravirus. The virus was found to be mechanically transmissible to Nicotiana benthamiana. Sequence alignment and phylogenetic analyses of the virus support the proposal of creating a new species in the genus Emaravirus for which the name lilac chlorotic ringspot-associated virus (LiCRaV) is proposed.

Key words: LiCRaV , lilac , Fimoviridae , Emaravirus

. [J]. Journal of Integrative Agriculture, 2020, 19(8): 2064-2071.

WANG Ya-qin, SONG Yu, CAO Meng-ji, CHENG Qi, WU Jian-xiang, HU Tao. Identification of a novel emaravirus infecting lilac through next-generation sequencing[J]. Journal of Integrative Agriculture, 2020, 19(8): 2064-2071.

参考文献

Di Bello P L, Laney A G, Druciarek T, Ho T, Gergerich R C, Keller K E, Martin R R, Tzanetakis I E. 2016. A novel emaravirus is associated with redbud yellow ringspot disease. Virus Research, 222, 41–47.
Bruenn J A. 2003. A structural and primary sequence comparison of the viral RNA-dependent RNA polymerases. Nucleic Acids Research, 31, 1821–1829.
Condit I, Horne W. 1933. A mosaic of the fig in California. Phytopathology, 23, 887–896.
Duijsings D, Kormelink R, Goldbach R. 2001. In vivo analysis of the TSWV cap-snatching mechanism: Single base complementarity and primer length requirements. The EMBO Journal, 20, 2545–2452.
Elbeaino T, Digiaro M, Martelli G P. 2009. Complete nucleotide sequence of four RNA segments of fig mosaic virus. Archives of Virology, 154, 1719–1727.
Elbeaino T, Digiaro M, Mielke-Ehret N, Muehlbach H P, Martelli G P. 2018. ICTV virus taxonomy profile: Fimoviridae. Journal of General Virology, 99, 1478–1479.
Elbeaino T, Digiaro M, Uppala M, Sudini H. 2014. Deep sequencing of pigeonpea sterility mosaic virus discloses five RNA segments related to emaraviruses. Virus Research, 188, 27–31.
Elbeaino T, Digiaro M, Uppala M, Sudini H. 2015. Deep sequencing of dsRNAs recovered from mosaic-diseased pigeonpea reveals the presence of a novel emaravirus: Pigeonpea sterility mosaic virus 2. Archives of Virology, 160, 2019–2029.
Flock R A. 1955. Transmission of fig mosaic by eriophyid mite Aceria ficus. Phytopathology, 45, 52–54.
Hassan M, Di Bello P L, Keller K E, Martin R R, Sabanadzovic S, Tzanetakis I E. 2017. A new, widespread emaravirus discovered in blackberry. Virus Research, 235, 1–5.
Kikkert M, Van Lent J, Storms M, Bodegom P, Kormelink R, Goldbach R. 1999. Tomato spotted wilt virus particle morphogenesis in plant cells. Journal of Virology, 73, 2288–2297.
Kulkarni N, Kumar P, Muniyappa V, Jones A, Reddy A. 2002. Transmission of Pigeon pea sterility mosaic virus by the eriophyid mite, Aceria cajani (Acari: Arthropoda). Plant Disease, 86, 1297–1302.
Laney A G, Keller K E, Martin R R, Tzanetakis I E. 2011. A discovery 70 years in the making: Characterization of the Rose rosette virus. Journal of General Virology, 92, 1727–1732.
Lukashevich I S, Djavani M, Shapiro K, Sanchez A, Ravkov E, Nichol S T, Salvato M S. 1997. The Lassa fever virus L gene: nucleotide sequence, comparison, and precipitation of a predicted 250 kDa protein with monospecific antiserum. Journal of General Virology, 78, 547–551.
McGavin W J, Mitchell C, Cock P J, Wright K M, MacFarlane S A. 2012. Raspberry leaf blotch virus, a putative new member of the genus Emaravirus, encodes a novel genomic RNA. Journal of General Virology, 93, 430–437.
Melcher U. 2000. The ‘30K’ superfamily of viral movement proteins. Journal of General Virology, 81, 257–266.
Mielke N, Mühlbach H P. 2007. A novel, multipartite, negative-strand RNA virus is associated with the ringspot disease of European mountain ash (Sorbus aucuparia L.). Journal of General Virology, 88, 1337–1346.
Mielke-Ehret N, Mühlbach H P. 2012. Emaravirus: A novel genus of multipartite, negative strand RNA plant viruses. Viruses, 4, 1515–1536.
Mielke-Ehret N, Thoma J, Schlatermund N, Mühlbach H P. 2010. Detection of European mountain ash ringspot-associated virus-specific RNA and protein P3 in the pear leaf blister mite Phytoptus pyri (Eriophyidae). Archives of Virology, 155, 987–991.
Patil B L, Kumar P L. 2015. Pigeonpea sterility mosaic virus: A legume-infecting Emaravirus from South Asia. Molecular Plant Pathology, 16, 775–786.
Scotto-Lavino E, Du G, Frohman M A. 2006. 5´ end cDNA amplification using classic RACE. Nature Protocols, 1, 2555–2562.
Tatineni S, McMechan A J, Wosula E N, Wegulo S N, Graybosch R A, French R, Hein G L. 2014. An eriophyid mite-transmitted plant virus contains eight genomic RNA segments with unusual heterogeneity in the nucleocapsid protein. Journal of Virology, 15, 11834–11845.
Yu C, Karlin D G, Lu Y, Wright K, Chen J, MacFarlane S. 2013. Experimental and bioinformatic evidence that raspberry leaf blotch emaravirus P4 is a movement protein of the 30K superfamily. Journal of General Virology, 94, 2117–2128.
Zheng Y, Navarro B, Wang G, Wang Y, Yang Z, Xu W, Zhu C, Wang L, Serio F D, Hong N. 2017. Actinidia chlorotic ringspot-associated virus: A novel emaravirus infecting kiwifruit plants. Molecular Plant Pathology, 18, 569–581.

Identification of a novel emaravirus infecting lilac through next-generation sequencing

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics