Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (7): 1356-1366.DOI: 10.1016/S2095-3119(14)60849-2

• 论文 • 上一篇    下一篇

A larval specific OBP able to bind the major female sex pheromone component in Spodoptera exigua (Hübner)

 JIN Rong, LIU Nai-yong, LIU Yan, DONG Shuang-lin   

  1. Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.China
  • 收稿日期:2014-04-09 出版日期:2015-07-03 发布日期:2015-07-06
  • 通讯作者: DONG Shuang-lin, Tel: +86-25-84399062,E-mail: sldong@njau.edu.cn
  • 基金资助:

    This work was supported by a grant from the National Natural Science Foundation of China (31372264) and the Special Fund for Agro-Scientific Research in the Public Interest,China (201203036).

A larval specific OBP able to bind the major female sex pheromone component in Spodoptera exigua (Hübner)

 JIN Rong, LIU Nai-yong, LIU Yan, DONG Shuang-lin   

  1. Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education/College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, P.R.China
  • Received:2014-04-09 Online:2015-07-03 Published:2015-07-06
  • Contact: DONG Shuang-lin, Tel: +86-25-84399062,E-mail: sldong@njau.edu.cn
  • Supported by:

    This work was supported by a grant from the National Natural Science Foundation of China (31372264) and the Special Fund for Agro-Scientific Research in the Public Interest,China (201203036).

摘要: Odorant binding proteins (OBPs) in insects are postulated to solubilize and transport the hydrophobic odorants across the hydrophilic antennal lymph to the olfactory receptors (ORs) located on the dendrite membrane of the sensory neurons. OBPs in adult insects have been intensively reported, but those in larvae are rarely addressed. In our study, a full-length OBP cDNA, namely SexiOBP13, was cloned by RT-PCR and RACE strategy from the heads of Spodoptera exigua larvae. The quantitative real-time PCR (qPCR) measurement indicated that SexiOBP13 was highly expressed in larval head, but very low in other parts of larva and was not detected in any tissues of adult. The binding affinities of SexiOBP13 to plant volatiles and female sex pheromone components were measured by competitive binding assays. Interestingly, SexiOBP13 displayed a high binding affinity (Ki=3.82 μmol L–1) to Z9,E12–14:Ac, the major sex pheromone component of S. exigua, while low affinities to the tested host plant volatiles (Ki>27 μmol L–1). The behavioral tests further confirmed that Z9,E12–14:Ac was indeed active to elicit the behavioral activity of the third instar larvae of S. exigua. Taken together, our results suggest that SexiOBP13 may play a role in reception of female sex pheromone in S. exigua larvae. The ecological significance of the larvae preference to the adult female sex pheromone was discussed.

关键词: odorant binding protein , female sex pheromone , larval specificity , binding affinity , behavioral response

Abstract: Odorant binding proteins (OBPs) in insects are postulated to solubilize and transport the hydrophobic odorants across the hydrophilic antennal lymph to the olfactory receptors (ORs) located on the dendrite membrane of the sensory neurons. OBPs in adult insects have been intensively reported, but those in larvae are rarely addressed. In our study, a full-length OBP cDNA, namely SexiOBP13, was cloned by RT-PCR and RACE strategy from the heads of Spodoptera exigua larvae. The quantitative real-time PCR (qPCR) measurement indicated that SexiOBP13 was highly expressed in larval head, but very low in other parts of larva and was not detected in any tissues of adult. The binding affinities of SexiOBP13 to plant volatiles and female sex pheromone components were measured by competitive binding assays. Interestingly, SexiOBP13 displayed a high binding affinity (Ki=3.82 μmol L–1) to Z9,E12–14:Ac, the major sex pheromone component of S. exigua, while low affinities to the tested host plant volatiles (Ki>27 μmol L–1). The behavioral tests further confirmed that Z9,E12–14:Ac was indeed active to elicit the behavioral activity of the third instar larvae of S. exigua. Taken together, our results suggest that SexiOBP13 may play a role in reception of female sex pheromone in S. exigua larvae. The ecological significance of the larvae preference to the adult female sex pheromone was discussed.

Key words: odorant binding protein , female sex pheromone , larval specificity , binding affinity , behavioral response