Isolation and identification of Serratia marcescens Ha1 and herbicidal activity of Ha1 ‘pesta’ granular formulation

doi:10.1016/S2095-3119(14)60967-9

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (7): 1348-1355.DOI: 10.1016/S2095-3119(14)60967-9

Isolation and identification of Serratia marcescens Ha1 and herbicidal activity of Ha1 ‘pesta’ granular formulation

YANG Juan, WANG Wei, YANG Peng, TAO Bu, YANG Zheng, ZHANG Li-hui, DONG Jin-gao

Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding 071001, P.R.China

收稿日期:2014-09-30 出版日期:2015-07-03 发布日期:2015-07-06
通讯作者: ZHANG Li-hui,Tel: +86-312-7520235, E-mail: zhanglihui@hebau.edu.cn; DONG Jin-gao, Tel: +86-312-7528266, E-mail: dongjingao@126.com
作者简介:YANG Juan, E-mail: yangjuan018@126.com; WANG Wei,-mail: 727728910@qq.com;* These authors contributed equally to this study.
基金资助:
This research was supported by the grants from the National High Technology Research and Development Program of China (2011AA10A206), the China Agriculture Research System (CARS-02) and the Science and Technology Support Program of Hebei, China (12220301D).

Isolation and identification of Serratia marcescens Ha1 and herbicidal activity of Ha1 ‘pesta’ granular formulation

YANG Juan, WANG Wei, YANG Peng, TAO Bu, YANG Zheng, ZHANG Li-hui, DONG Jin-gao

Mycotoxin and Molecular Plant Pathology Laboratory, Agricultural University of Hebei, Baoding 071001, P.R.China

Received:2014-09-30 Online:2015-07-03 Published:2015-07-06
Contact: ZHANG Li-hui,Tel: +86-312-7520235, E-mail: zhanglihui@hebau.edu.cn; DONG Jin-gao, Tel: +86-312-7528266, E-mail: dongjingao@126.com
About author:YANG Juan, E-mail: yangjuan018@126.com; WANG Wei,-mail: 727728910@qq.com;* These authors contributed equally to this study.
Supported by:
This research was supported by the grants from the National High Technology Research and Development Program of China (2011AA10A206), the China Agriculture Research System (CARS-02) and the Science and Technology Support Program of Hebei, China (12220301D).

摘要/Abstract

摘要： A total of 479 bacterial strains were isolated from brine (Bohai, Qinhuangdao City, Hebei Province, China). Bioassay results indicated that 4 strains named Ha1, Ha17, Ha38, and Ha384 had herbicidal activity. And strain Ha1 had the highest effective herbicidal activity. As a result, this study aims to identify strain Ha1, characterize its physiological and biological activities, evaluate the herbicidal activity of its metabolites, and develop a ‘pesta’ formulation and assess its effectiveness on Digitaria sanguinalis. Ha1 was identified as Serratia marcescens based on 16S rDNA sequencing. This strain has a flagellum, a diameter of 0.5 to 0.8 μm, and a length of 0.9 to 2.0 μm. The indole test shows positive results, and the catalase enzyme exhibits strong positive reactions. Results further showed that the inhibitory concentration (IC50) of the crude extracts to D. sanguinalis radicula and coleoptile were 3.332 and 2.828 mg mL–1, respectively. Both the suppression of D. sanguinalis and the cell viability of the Ha1 formulation in ‘pesta’ were higher when stored at 4°C than at (25±2)°C. These results indicated that S. marcescens Ha1 can potentially be used as a biocontrol agent against D. sanguinalis.

关键词: Serratia marcescens , bioherbicide , biocontrol , pesta

Abstract: A total of 479 bacterial strains were isolated from brine (Bohai, Qinhuangdao City, Hebei Province, China). Bioassay results indicated that 4 strains named Ha1, Ha17, Ha38, and Ha384 had herbicidal activity. And strain Ha1 had the highest effective herbicidal activity. As a result, this study aims to identify strain Ha1, characterize its physiological and biological activities, evaluate the herbicidal activity of its metabolites, and develop a ‘pesta’ formulation and assess its effectiveness on Digitaria sanguinalis. Ha1 was identified as Serratia marcescens based on 16S rDNA sequencing. This strain has a flagellum, a diameter of 0.5 to 0.8 μm, and a length of 0.9 to 2.0 μm. The indole test shows positive results, and the catalase enzyme exhibits strong positive reactions. Results further showed that the inhibitory concentration (IC50) of the crude extracts to D. sanguinalis radicula and coleoptile were 3.332 and 2.828 mg mL–1, respectively. Both the suppression of D. sanguinalis and the cell viability of the Ha1 formulation in ‘pesta’ were higher when stored at 4°C than at (25±2)°C. These results indicated that S. marcescens Ha1 can potentially be used as a biocontrol agent against D. sanguinalis.

Key words: Serratia marcescens , bioherbicide , biocontrol , pesta

YANG Juan, WANG Wei, YANG Peng, TAO Bu, YANG Zheng, ZHANG Li-hui, DONG Jin-gao. Isolation and identification of Serratia marcescens Ha1 and herbicidal activity of Ha1 ‘pesta’ granular formulation[J]. Journal of Integrative Agriculture, 2015, 14(7): 1348-1355.

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Isolation and identification of Serratia marcescens Ha1 and herbicidal activity of Ha1 ‘pesta’ granular formulation

Isolation and identification of Serratia marcescens Ha1 and herbicidal activity of Ha1 ‘pesta’ granular formulation

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