Effect of Drought Stress During Flowering Stage on Starch Accumulation and Starch Synthesis Enzymes in Sorghum Grains

doi:10.1016/S2095-3119(13)60694-2

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (11): 2399-2406.DOI: 10.1016/S2095-3119(13)60694-2

Effect of Drought Stress During Flowering Stage on Starch Accumulation and Starch Synthesis Enzymes in Sorghum Grains

YI Bing, ZHOU Yu-fei, GAO Ming-yue, ZHANG Zhuang, HAN Yi, YANG Guang-dong, XU Wenjuan, HUANG Rui-dong

1、College of Agronomy, Shenyang Agricultural University, Shenyang 110866, P.R.China
2、Keshan Research Institute, Heilongjiang Institute of Agricultural Sciences, Qiqihar 161606, P.R.China

收稿日期:2012-11-17 出版日期:2014-11-06 发布日期:2014-11-14
通讯作者: HUANG Rui-dong, Tel: +86-24-88487135, E-mail: r_huang@126.com
作者简介:YI Bing, E-mail: yibing8749@126.com
基金资助:
This work was supported by the Earmarked Fund for Modern Agro-Industry Technology Research System, China.

Effect of Drought Stress During Flowering Stage on Starch Accumulation and Starch Synthesis Enzymes in Sorghum Grains

YI Bing, ZHOU Yu-fei, GAO Ming-yue, ZHANG Zhuang, HAN Yi, YANG Guang-dong, XU Wenjuan, HUANG Rui-dong

1、College of Agronomy, Shenyang Agricultural University, Shenyang 110866, P.R.China
2、Keshan Research Institute, Heilongjiang Institute of Agricultural Sciences, Qiqihar 161606, P.R.China

Received:2012-11-17 Online:2014-11-06 Published:2014-11-14
Contact: HUANG Rui-dong, Tel: +86-24-88487135, E-mail: r_huang@126.com
About author:YI Bing, E-mail: yibing8749@126.com
Supported by:
This work was supported by the Earmarked Fund for Modern Agro-Industry Technology Research System, China.

摘要/Abstract

摘要： Starch content is a key factor affecting sorghum grain quality. The research of sorghum grain starch accumulation and the related synthesis enzyme activities has great significance for understanding the mechanisms of starch metabolisms. The differences between a high and a low starch content sorghum hybrids (Tieza 17 and Liaoza 11, respectively) in grain starch accumulation and the related synthesis enzyme activities were assessed following imposition of water stress during flowering stage. The total starch, amylase and amylopectin accumulation all decreased at the mid-late stage of grain filling under drought stress during flowering stage. The maximum and mean accumulation rates also decreased. During grain filling, soluble starch synthase (SSS), granule-bound starch synthase (GBSS), starch branching enzyme (SBE), and starch debranching enzymes (DBE) activities were all affected, though differently. Drought stress reduced starch accumulation in a larger extent for Tieza 17 than Liaoza 11. Drought stress during flowing stage reduced starch synthesis enzyme activities, thus reducing starch accumulation in grains, and the differences between starch components were also demonstrated under drought stress.

关键词: sorghum , drought stress , starch accumulation , enzyme activity

Abstract: Starch content is a key factor affecting sorghum grain quality. The research of sorghum grain starch accumulation and the related synthesis enzyme activities has great significance for understanding the mechanisms of starch metabolisms. The differences between a high and a low starch content sorghum hybrids (Tieza 17 and Liaoza 11, respectively) in grain starch accumulation and the related synthesis enzyme activities were assessed following imposition of water stress during flowering stage. The total starch, amylase and amylopectin accumulation all decreased at the mid-late stage of grain filling under drought stress during flowering stage. The maximum and mean accumulation rates also decreased. During grain filling, soluble starch synthase (SSS), granule-bound starch synthase (GBSS), starch branching enzyme (SBE), and starch debranching enzymes (DBE) activities were all affected, though differently. Drought stress reduced starch accumulation in a larger extent for Tieza 17 than Liaoza 11. Drought stress during flowing stage reduced starch synthesis enzyme activities, thus reducing starch accumulation in grains, and the differences between starch components were also demonstrated under drought stress.

Key words: sorghum , drought stress , starch accumulation , enzyme activity

YI Bing, ZHOU Yu-fei, GAO Ming-yue, ZHANG Zhuang, HAN Yi, YANG Guang-dong, XU Wenjuan, HUANG Rui-dong. Effect of Drought Stress During Flowering Stage on Starch Accumulation and Starch Synthesis Enzymes in Sorghum Grains[J]. Journal of Integrative Agriculture, 2014, 13(11): 2399-2406.

参考文献

Ahmadi A, Baker D A. 2001. The effect of water stress on theactivities of key regulatory enzymes of the sucrose to starchpathway in wheat. Plant Growth Regulation, 35, 81-91

Alison M S. 1999. Making starch. Current Opinion in PlantBiology, 2, 223-229

Chen B X, Li J H, Gao S J. 2010. Breeding and application ofJiza 123 sorghum hybrid containing high starch. Seed, 8, 126-127 (in Chinese)

Cheng F M, Jiang D A, Wu P, Shi C H. 2001. The dynamicchange of starch synthes is enzymes during the grain fillingstage and effects of temperature upon it. Acta AgronomicaSinica, 27, 201-206 (in Chinese)

Cheng Q J, Zhang F Y, Zhao W J, Chang Y H, Tian C H, GaoH Y, Gao P, Zhang G X. 2010. Screening and utilization ofhigh starch content sorghum. Chinese Agricultural ScienceBulletin, 26, 103-106. (in Chinese)

Chu P F, Yu Z W, Wang X Y, Wu T H, Wang X Z. 2009.Effects of irrigation amount on grain sarch content, starchsynthase activity and water use efficiency in wheat. ActaAgronomica Sinica, 35, 324-333. (in Chinese)

Dai Z M, Wang Z L, Zhang M, Li W Y, Yan S H, Cai R G, YinY P. 2008. Starch accumulation and activities of enzymesinvolved in starch synthesis in grains of wheat grown underirrigation and rain-fed conditions. Scientia AgriculturaSinica, 41, 687-694. (in Chinese)

Duffus C M. 1992. Control of starch biosynthesis in developingcereal grains. Biochemical Society Transactions, 20, 13-18

Emes M J, Bowsher C G, Hedley C, Hedley C, Burrell M M,Scrase-Field E S E, Tetlow I J. 2003. Starch synthesis andcarbon partitioning in developing endosperm. Journal ofExperiental Botany, 54, 569-575

Gao X, Xiao M J, Zhou Y F, Xu W J, Huang R D. 2009. Effectsof planting density on starch accumulation in sorghumgrains. Crops, 6, 35-37

Genschel U, Abel G, Lörz H, Lütticke S. 2002. The sugary-typeisoamylase in wheat: Tissue distribution and subcellularlocalization. Planta, 214, 813-820

He Z F. 1985. Grain Quality and Its Analysis Technology.Agricultural Press, Beijing. pp. 274-294(in Chinese)

Huang R D, Yu Y, Xiao M J, Xu W J, Zhou Y F. 2009.Effects of different phosphorus and potassium fertilizerapplication on starch accumulation in sorghum grains.Crops, 12, 29-32

James M G, Denyer K, Myers A M. 2003. Starch synthesis inthe cereal endosperm. Current Opinion in Plant Biology,6, 215-222

Jiang D, Cao W X, Dai T B, Jing Q. 2003. Activities of keyenzymes for starch synthesis in relation to growth ofsuperior and inferior grains on winter wheat (Triticumaestivum L.) spike. Plant Growth Regulation, 41, 247-257

Kubo A, Fujita N, Harada K, Matsuda T, Satoh H, NakamuraY. 1999. The starch debranching enzymes isoamylase andpulluanase are both involved in amylopectin biosynthesisin rice endosperm. Plant Physiology, 121, 399-409

Li C, Xiao M J, Zhou Y F, Xu W J, Huang R D. 2009. Effectof sowing dates on the starch contents in sorghum grains.Journal of Shenyang Agricultural University, 40, 708-711

(in Chinese)Li T G, Shen B, Chen N, Luo Y K. 1997. Effect of Q-enzymeon the chalkiness formation of rice grain. Acta AgronomicaSinica, 23, 338-344 (in Chinese)

Li Z Y, Sun F, Xu S M, Chu X S, Mukai Y, Yamamoto M, AliS, Rampling L, Kosar-Hashemi B, Rahman S, Morell MK. 2003. The structural organisation of the gene encodingclass II starch synthase of wheat and barley and theevolution of the genes encoding starch synthases in plants.Funct Integr Genomic, 3, 76-85

Liu Y, Wang J F, Wang S, Zhu T D. 2010. Comparison ofstarch accumulation characteristics in maize grains withdifferent starch contents. Shandong Agricultural Sciences,8, 34-37 (in Chinese)

Ma J, Ming D F, Ma W B, Xu F Y. 2005. Changes in starchaccumulation and activity of enzymes associated withstarch synthesis under different N supplying date. ScientiaAgricultura Sinica, 38, 290-296. (in Chinese)

Morell M K, Myers A M. 2005. Towards the rational designof cereal starches. Current Opinion in Plant Biology, 8,204-210

Nakamura Y, Kuki K, Park S Y, Ohya T. 1989. Carbohydratemetabolism in the developing endosperm of rice grains.Plant Cell Physiology, 30, 833-839

Nakamura Y, Umemoto T, Ogata N, Kuboki Y, Yano M,Sasaki T. 1996. Starch debranching enzyme (R-enzyme orpullulanase) from developing rice endosperm: Purification,cDNA and chromosomal localization of the gene. Planta,199, 209-218

Patron N J, Smith A M, Fahy B F, Hylton C M, Naldrett MJ, Rossnagel B G, Denyer K. 2002. The altered pattern ofamylose accumulation in the endosperm of low-amylosebarley cultivars is attributable to a single mutant alleleof granule-bound starch synthase I with a deletion in the5´-non-coding region. Plant Physiology, 130, 190-198

Tan C X, Feng C N, Guo W S, Zhu X K, Li C Y, Peng Y X.2011. Different in expression of starch synthase gene andstarch synthesis in the grain of different wheat cultivars.Journal of Triticeae Corps, 31, 1063-1070 (in Chinese)

Wang H, Ma J, Li X Y, Zhang R P. 2009. Effects of waterstress on grain filling and activities of enzymes involvedin starch synthesis in rice. Scientia Agricultura Sinica, 42,1550-1558. (in Chinese)

Wang W J. 2007. Effect of different soil types on the dynamicchanges of starch accumulation and enzymes activitiesinvolved in ZM 9023 kernel starch synthesis. ScientiaAgricultura Sinica, 40, 204-211. (in Chinese)

Wang Z B, Yin Y G, Cao L P, Wang Z M, Hou R R, Li W H,Qi J C. 2010. Study on activity of enzymes involved instarch synthesis and accumulation in grains of two typesof wheat cultivars. Journal of Triticeae Crops, 30, 259-265. (in Chinese)

Worch S, Rajesh K, Harshavardhan V T, Pietsch C, Korzun V,Kuntze L, Börner A, Wobus U, Röder M S, SreenivasuluN. 2011. Haplotyping, linkage mapping and expressionanalysis of barley genes regulated by terminal droughtstress influencing seed quality. BMC Plant Biology, 4, 1.

Yu Y, Huang R D, Zhao S W, Jiang W C. 2008. Effect ofnitrogen application on starch accumulation in sorghumgrains. Crops, 15, 20-24

Zhang H Y, Dong S T, Gao R Q. 2006. The study progressin plant starch. Journal of the Chinese Cereals and OilsAssociation, 21, 41-46. (in Chinese)

[1]	ZHANG Su-jiang, Abdul Shakoor Chaudhry, Diky Ramdani, Amerjan Osman, GUO Xue-feng, Grant Raymond Edwards, Long Cheng. Chemical composition and in vitro fermentation characteristics of high sugar forage sorghum as an alternative to forage maize for silage making in Tarim Basin, China[J]. Journal of Integrative Agriculture, 2016, 15(1): 175-182.
[2]	MA Ping, BAI Tuan-hui, WANG Xiao-qian, MA Feng-wang. Effects of light intensity on photosynthesis and photoprotective mechanisms in apple under progressive drought[J]. Journal of Integrative Agriculture, 2015, 14(9): 1755-1766.
[3]	ZHANG Fan, CHEN Xun-ji, WANG Jian-hua, ZHENG Jun. Overexpression of a maize SNF-related protein kinase gene, ZmSnRK2.11, reduces salt and drought tolerance in Arabidopsis[J]. Journal of Integrative Agriculture, 2015, 14(7): 1229-1241.
[4]	MA Ping, BAI Tuan-hui and MA Feng-wang. Effects of progressive drought on photosynthesis and partitioning of absorbed light in apple trees[J]. Journal of Integrative Agriculture, 2015, 14(4): 681-690.
[5]	ZHANG Yu-feng, HUANG Xiu-wen, WANG Li-li, WEI Liu, WU Zhi-hui, YOU Ming-shan , LI Bao-yun. Proteomic Analysis of Wheat Seed in Response to Drought Stress[J]. Journal of Integrative Agriculture, 2014, 13(5): 919-925.
[6]	SHAO Xing-hua , ZHENG Jian-wei. Soil Organic Carbon, Black Carbon, and Enzyme Activity Under Long- Term Fertilization[J]. Journal of Integrative Agriculture, 2014, 13(3): 517-524.
[7]	TIAN Jing-shan, HU Yuan-yuan, GAN Xiu-xia, ZHANG Ya-li, HU Xiao-bing, GOU Ling, LUO Hong-hai. Effects of Increased Night Temperature on Cellulose Synthesis and the Activity of Sucrose Metabolism Enzymes in Cotton Fiber[J]. Journal of Integrative Agriculture, 2013, 12(6): 979-988.
[8]	CHANG Jin-hua, CUI Jiang-hui, XUE Wei, ZHANG Qing-wen. Identification of Molecular Markers for a Aphid Resistance Gene in Sorghum and Selective Efficiency Using These Markers[J]. Journal of Integrative Agriculture, 2012, 12(7): 1086-1092.
[9]	ZHAO Yan-dong, SUN Yu-rui, CAI Xiang, LIU He, Peter Schulze Lammers. Identify Plant Drought Stress by 3D-Based Image[J]. Journal of Integrative Agriculture, 2012, 12(7): 1207-1211.
[10]	GUAN Guan, TU Shu-xin, YANG Jun-cheng, ZHANG Jian-feng, YANG Li. A Field Study on Effects of Nitrogen Fertilization Modes on Nutrient Uptake,Crop Yield and Soil Biological Properties in Rice-Wheat Rotation System[J]. Journal of Integrative Agriculture, 2011, 10(8): 1254-1261.
[11]	ZHANG Bin, LIU Huai, Helen Hull-Sanders, WANG Jin-jun. Effect of Host Plants on Development, Fecundity and Enzyme Activity of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae)[J]. Journal of Integrative Agriculture, 2011, 10(8): 1232-1240.
[12]	FU Guan-fu, SONG Jian, XIONG Jie, LI Yu-rong, CHEN Hui-zhe, LE Ming-kai and TAO Long-xing. Changes of Oxidative Stress and Soluble Sugar in Anthers Involve in Rice Pollen Abortion Under Drought Stress[J]. Journal of Integrative Agriculture, 2011, 10(7): 1016-1025.
[13]	Irakoze Pierre Claver, ZHOU Hui-ming, ZHANG Hai-hua, ZHU Ke-xue, LI Qin , Murekatete Nicole. The Effect of Soaking with Wooden Ash and Malting upon Some Nutritional Properties of Sorghum Flour Used for Impeke, a Traditional Burundian Malt- Based Sorghum Beverage[J]. Journal of Integrative Agriculture, 2011, 10(11): 1801-1811.

Effect of Drought Stress During Flowering Stage on Starch Accumulation and Starch Synthesis Enzymes in Sorghum Grains

Effect of Drought Stress During Flowering Stage on Starch Accumulation and Starch Synthesis Enzymes in Sorghum Grains

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 13

编辑推荐

Metrics