Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents

doi:10.1016/S2095-3119(13)60583-3

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (1): 54-62.DOI: 10.1016/S2095-3119(13)60583-3

Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents

TAN Xiao-li, ZHENG Xiang-feng, ZHANG Zhi-yan, WANG Zheng, XIA Heng-chuan, LU Changming, GU Shou-lai

1.Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, P.R.China
2.Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, P.R.China

收稿日期:2013-01-06 出版日期:2014-01-01 发布日期:2014-01-04
通讯作者: TAN Xiao-li, Fax: +86-511-88791923, E-mail: xltan@ujs.edu.cn; GU Shou-lai, E-mail: gushoulai1987@126.com
作者简介:TAN Xiao-li, Fax: +86-511-88791923, E-mail: xltan@ujs.edu.cn
基金资助:
This work was supported by the Natural Science Foundation of China (31271760) and the Opening Funds of the Key Laboratory of Biology and Genetic Improvement of Oilcrops, Ministry of Agriculture, China.

Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents

TAN Xiao-li, ZHENG Xiang-feng, ZHANG Zhi-yan, WANG Zheng, XIA Heng-chuan, LU Changming, GU Shou-lai

1.Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, P.R.China
2.Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, P.R.China

Received:2013-01-06 Online:2014-01-01 Published:2014-01-04
Contact: TAN Xiao-li, Fax: +86-511-88791923, E-mail: xltan@ujs.edu.cn; GU Shou-lai, E-mail: gushoulai1987@126.com
About author:TAN Xiao-li, Fax: +86-511-88791923, E-mail: xltan@ujs.edu.cn
Supported by:
This work was supported by the Natural Science Foundation of China (31271760) and the Opening Funds of the Key Laboratory of Biology and Genetic Improvement of Oilcrops, Ministry of Agriculture, China.

摘要/Abstract

摘要： Long-chain acyl-Coenzyme A (CoA) synthetases (LACSs) catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol (TAG) biosynthesis and fatty acid β-oxidation. Here, we report the characterization of a LACS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The cloned gene BnLACS4 could complement a LACS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassica napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum (ER), the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.

关键词: Brasscia napus , LACS , lipid , subcellular localization , yeast

Abstract: Long-chain acyl-Coenzyme A (CoA) synthetases (LACSs) catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol (TAG) biosynthesis and fatty acid β-oxidation. Here, we report the characterization of a LACS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The cloned gene BnLACS4 could complement a LACS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassica napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum (ER), the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.

Key words: Brasscia napus , LACS , lipid , subcellular localization , yeast

TAN Xiao-li, ZHENG Xiang-feng, ZHANG Zhi-yan, WANG Zheng, XIA Heng-chuan, LU Changming, GU Shou-lai. Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents[J]. Journal of Integrative Agriculture, 2014, 13(1): 54-62.

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Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents

Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents

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