Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (4): 788-799.DOI: 10.1016/S2095-3119(14)60773-5

• 论文 • 上一篇    

Interactions of water and nitrogen addition on soil microbial community composition and functional diversity depending on the inter-annual precipitation in a Chinese steppe

 SUN Liang-jie, QI Yu-chun, DONG Yun-she, HE Ya-ting, PENG Qin, LIU Xin-chao, JIA Jun-qiang, GUO Shu-fang, CAO Cong-cong   

  1. 1、Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, P.R.China
    2、University of Chinese Academy of Sciences, Beijing 100049, P.R.China
    3、Key Laboratory of Crop Nutrition and Fertilization, Ministry of Agriculture/Institute of Agricultural Resources and RegionalPlanning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
  • 收稿日期:2014-02-20 出版日期:2015-04-01 发布日期:2015-04-10
  • 通讯作者: DONG Yun-she, Tel: +86-10-64856500,E-mail: dongys@igsnrr.ac.cn
  • 作者简介:SUN Liang-jie, E-mail: sunlj-2007@hotmail.com;
  • 基金资助:

    This work was financially supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-EW-302), the National Natural Science Foundation of China (41330528, 41373084 and 41203054), and the Special Fund for Agro-Scientific Research in the Public Interest, China (201203012).

Interactions of water and nitrogen addition on soil microbial community composition and functional diversity depending on the inter-annual precipitation in a Chinese steppe

 SUN Liang-jie, QI Yu-chun, DONG Yun-she, HE Ya-ting, PENG Qin, LIU Xin-chao, JIA Jun-qiang, GUO Shu-fang, CAO Cong-cong   

  1. 1、Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, P.R.China
    2、University of Chinese Academy of Sciences, Beijing 100049, P.R.China
    3、Key Laboratory of Crop Nutrition and Fertilization, Ministry of Agriculture/Institute of Agricultural Resources and RegionalPlanning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
  • Received:2014-02-20 Online:2015-04-01 Published:2015-04-10
  • Contact: DONG Yun-she, Tel: +86-10-64856500,E-mail: dongys@igsnrr.ac.cn
  • About author:SUN Liang-jie, E-mail: sunlj-2007@hotmail.com;
  • Supported by:

    This work was financially supported by the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-EW-302), the National Natural Science Foundation of China (41330528, 41373084 and 41203054), and the Special Fund for Agro-Scientific Research in the Public Interest, China (201203012).

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摘要： Water and nitrogen are primary limiting factors in semiarid grassland ecosystems. Our knowledge is still poor regarding the interactive effects of water and N addition on soil microbial communities, although this information is crucial to reveal the mechanisms of the terrestrial ecosystem response to global changes. We addressed this problem by conducting a field experiment with a 15% surplus of the average rainfall under three levels of N addition (50, 100, and 200 kg N ha–1 yr–1) in two consecutive years in Inner Mongolia, China. Microbial community composition and functional diversity were analyzed based on phospholipid fatty acids (PLFA) and BIOLOG techniques, respectively. The results showed that water addition did not affect the soil microbial community composition, but much more yearly precipitation generally decreased the PLFA concentration, which implied a fast response of soil microbes to changes of water condition. Soil fungi was depressed only by N addition at the high level (200 kg N ha–1 yr–1) and without hydrologic leaching, while Gram-negative bacteria was suppressed probably by plant competition at high level N addition but with hydrologic leaching. The study found unilateral positive/negative interactions between water and N addition in affecting soil microbial community, however, climate condition (precipitation) could be a significant factor in disturbing the interactions. This study highlighted that: (1) The sustained effect of pulsed water addition was minimal on the soil microbial community composition but significant on the microbial community functional diversity and (2) the complex interaction between water and N addition on soil microbial community related to the inter-annual variation of the climate and plant response.

关键词: water addition , nitrogen addition , phospholipid fatty acid (PLFA) , BIOLOG-substrate utilization , semiarid steppe

Abstract: Water and nitrogen are primary limiting factors in semiarid grassland ecosystems. Our knowledge is still poor regarding the interactive effects of water and N addition on soil microbial communities, although this information is crucial to reveal the mechanisms of the terrestrial ecosystem response to global changes. We addressed this problem by conducting a field experiment with a 15% surplus of the average rainfall under three levels of N addition (50, 100, and 200 kg N ha–1 yr–1) in two consecutive years in Inner Mongolia, China. Microbial community composition and functional diversity were analyzed based on phospholipid fatty acids (PLFA) and BIOLOG techniques, respectively. The results showed that water addition did not affect the soil microbial community composition, but much more yearly precipitation generally decreased the PLFA concentration, which implied a fast response of soil microbes to changes of water condition. Soil fungi was depressed only by N addition at the high level (200 kg N ha–1 yr–1) and without hydrologic leaching, while Gram-negative bacteria was suppressed probably by plant competition at high level N addition but with hydrologic leaching. The study found unilateral positive/negative interactions between water and N addition in affecting soil microbial community, however, climate condition (precipitation) could be a significant factor in disturbing the interactions. This study highlighted that: (1) The sustained effect of pulsed water addition was minimal on the soil microbial community composition but significant on the microbial community functional diversity and (2) the complex interaction between water and N addition on soil microbial community related to the inter-annual variation of the climate and plant response.

Key words: water addition , nitrogen addition , phospholipid fatty acid (PLFA) , BIOLOG-substrate utilization , semiarid steppe