Lactic Acid Reduces LPS-Induced TNF- and IL-6 mRNA Levels Through Decreasing I B Phosphorylation

doi:10.1016/S2095-3119(13)60326-3

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (6): 1073-1078.DOI: 10.1016/S2095-3119(13)60326-3

Lactic Acid Reduces LPS-Induced TNF- and IL-6 mRNA Levels Through Decreasing I B Phosphorylation

XU Guang-yong, JIANG Jin-qi, WANG Ming, LI Jie, SU Jing-liang , REN Xiao-ming

1 Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, P.R.China
2 Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, P.R.China
3 College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China

收稿日期:2012-04-09 出版日期:2013-06-01 发布日期:2013-06-09
通讯作者: Correspondence REN Xiao-ming, Tel/Fax: +86-10-80795532, E-mail: xm-ren@163.com;SU Jing-liang, Tel/Fax: +86-10-62732312, E-mail: jinglsu@yahoo.com
作者简介:XU Guang-yong, Mobile: 15210922015, E-mail: 15210922015@139.com
基金资助:
This study was supported by the Natural Science Foundation of Beijing, China (6072007), and the Project for the Establishment and Development of Swine Disease Clinical Diagnosis, China (KM200910020002).

Lactic Acid Reduces LPS-Induced TNF- and IL-6 mRNA Levels Through Decreasing I B Phosphorylation

XU Guang-yong, JIANG Jin-qi, WANG Ming, LI Jie, SU Jing-liang , REN Xiao-ming

1 Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, P.R.China
2 Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, P.R.China
3 College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China

Received:2012-04-09 Online:2013-06-01 Published:2013-06-09
Contact: Correspondence REN Xiao-ming, Tel/Fax: +86-10-80795532, E-mail: xm-ren@163.com;SU Jing-liang, Tel/Fax: +86-10-62732312, E-mail: jinglsu@yahoo.com
About author:XU Guang-yong, Mobile: 15210922015, E-mail: 15210922015@139.com
Supported by:
This study was supported by the Natural Science Foundation of Beijing, China (6072007), and the Project for the Establishment and Development of Swine Disease Clinical Diagnosis, China (KM200910020002).

摘要/Abstract

摘要： This study explored the effects over time of lactic acid (LA) on I Bα phosphorylation and nuclear factor-kappa B (NF- B) p65 protein expression, and on tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) mRNA levels in rat intestinal mucosa microvascular endothelial cells (RIMMVECs) stimulated by lipopolysaccharide (LPS). I B , phosphorylated I B (p-I B ) and p65 protein levels were monitored by Western blot analysis, and TNF- and IL-6 mRNA levels were analyzed using real-time PCR. LA treatment reduced TNF- and IL-6 mRNA levels in LPS-stimulated RIMMVECs, with the greatest effect being after 3 h. The highest inhibitory effect of LA on I B phosphorylation to prevent activation of NFB was after 6 h. These results suggest that LA reduces TNF- and IL-6 mRNA levels through decreasing I B phosphorylation and blocking the dissociation of IKK complex, which prevents activation of NF- B.

关键词: lactic acid , TNF- , IL-6 , I B , NF- B

Abstract: This study explored the effects over time of lactic acid (LA) on I Bα phosphorylation and nuclear factor-kappa B (NF- B) p65 protein expression, and on tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) mRNA levels in rat intestinal mucosa microvascular endothelial cells (RIMMVECs) stimulated by lipopolysaccharide (LPS). I B , phosphorylated I B (p-I B ) and p65 protein levels were monitored by Western blot analysis, and TNF- and IL-6 mRNA levels were analyzed using real-time PCR. LA treatment reduced TNF- and IL-6 mRNA levels in LPS-stimulated RIMMVECs, with the greatest effect being after 3 h. The highest inhibitory effect of LA on I B phosphorylation to prevent activation of NFB was after 6 h. These results suggest that LA reduces TNF- and IL-6 mRNA levels through decreasing I B phosphorylation and blocking the dissociation of IKK complex, which prevents activation of NF- B.

Key words: lactic acid , TNF- , IL-6 , I B , NF- B

XU Guang-yong, JIANG Jin-qi, WANG Ming, LI Jie, SU Jing-liang , REN Xiao-ming. Lactic Acid Reduces LPS-Induced TNF- and IL-6 mRNA Levels Through Decreasing I B Phosphorylation[J]. Journal of Integrative Agriculture, 2013, 12(6): 1073-1078.

参考文献

[1]Ahn K S, Aggarwal B B. 2005. Transcription factor NFkappaB:asensor for smoke and stress signals. Annalsof the New York Academy of Sciences, 1056, 218-233

[2]le Bail O, Schmidt-Ullrich R, Israel A. 1993. Promoteranalysis of the gene encoding the IkappaB-alpha/MAD3 inhibitor of NF-kappa B: positive regulation bymembers of the rel/NF-kappa B family. EMBO Journal,12, 5043-5049

[3]Collart M A, Baeuerle P, Vassalli P. 1990. Regulation oftumor necrosis factor alpha transcription inmacrophages: involvement of four kappa B-like motifsand of constitutive and inducible forms of NF-kappa B.Molecular and Cellular Biochemistry, 10, 1498-1506

[4]Collins T, Read M A, Neish A S, Whitley M Z, Thanos D,Maniatis T. 1995. Transcriptional regulation ofendothelial cell adhesion molecules: NF-?B and cytokine-inducible enhancers. FASEB Journal, 9, 899-909

[5]Cordle S R, Donald R, Read M A, Hawiger J. 1993.Lipopolysaccharide induces phosphorylation of MAD3and activation of c-Rel and related NF-?B proteins inhuman monocytic THP-1 cells Journal of BiologicalChemistry, 268, 11803-11810

[6]Deshpande R, Khalili H, Pergolizzi R G, Michael S D, ChangM D. 1997. Estradiol down-regulates LPS-inducedcytokine production and NF-?B activation in murinemacrophages. American Journal of ReproductiveImmunology, 38, 46-54

[7]DiDonato J A, Hayakawa M, Rothwarf D M, Zandi E, KarinM. 1997. A cytokine-responsive IkappaB kinase thatactivates the transcription factor NF-kappaB. Nature,388, 548-554

[8]Eder K, Baffy N, Falus A, Fulop A K. 2009. The majorinflammatory Mediator interleukin-6 and obesity

[9]Inflammation Research, 58, 727-736

[10]Galien R, Evans H F, Garcia T. 1996. Involvement of CCAAT/enhancer-binding protein and nuclear factor-kappa Bbinding sites in interleukin-6 promoter inhibition byestrogens Molecular Endocrinology, 10, 713-722

[11]Hayden M S, Ghosh S. 2008. Shared principles in NF-kappaBsignaling pathways. Cell, 132, 344-362

[12]Hindryckx P, de Vos M, Jacques P, Ferdinande L, PeetersH, Olievier K, Bogaert S, Brinkman B, Vandenabeele P,Elewaut D, et al. 2010. Hydroxylase inhibition abrogatesTNF-a induced intestinal epithelial damage by hypoxiainduciblefactor-1-dependent repression of FADD TheJournal of Immunology, 185, 6306-6316

[13]Kishimoto T. 2010. IL-6: from its discovery to clinicalapplications International Immunology, 22, 347-352

[14]Kumar A, Takada Y, Boriek A M, Aggarwal B B. 2004. Nuclearfactor-kappaB: its role in health and disease. TheJournal of Molecular Medicine, 82, 434-448

[15]Liu J, Xue J Z, Zhu Z N, Hu G, Ren X M. 2011. Lactic acidinhibits NF-?B activation by lipopolysaccharide in ratintestinal mucosa microvascular endothelial cells.Agricultural Sciences in China, 10, 954-959

[16]Lee J M, Hwang K T, Jun W J, Park C S, Lee M Y. 2008.Antiinflammatory effect of lactic acid bacteria:inhibition of cyclooxygenase-2 by suppressing nuclearfactor-?B in Raw2647 macrophage cells. Journal ofMicrobiology and Biotechnology, 18, 1683-1688

[17]de Luc V, Frederic L. 2007. Bacteriocins from lactic acidbacteria: production, purification, and food applications.J o u r n a l o f M o l e c u l a r M i c r o b i o l o g y a n dBiotechnology, 13, 194-199

[18]Mercurio F, Zhu H, Murray B W, Shevchenko A, BennettB L, Li J. 1997. IKK-1 and IKK-2: cytokine-activatedIkappaB kinases essential for NF-kappaB activation Science, 278, 860-866

[19]Odrowaz-Sypniewska G. 2007. Markers of pro-inflammatoryand pro-thrombotic state in the diagnosis of metabolicsyndrome. Advances in Medical Sciences, 52, 246-250

[20]Sun D, Lytle C, O´Donnell M E. 1997. IL-6 secreted byastroglial cells regulates Na-K-Cl cotransport in brainmicrovessel endothelial cells American Journal ofPhysiology, 272, C1829-C1835.

[21]Tabruyn S P, Mémet S, Avé P, Verhaeghe C, Mayo K H,Struman I, Martial J A, Griffioen A W. 2009. NF-?Bactivation in endothelial cells is critical for the activityof angiostatic agents. Molecular Cancer Therapeutics,8, 2645-2654

[22]Zandi E, Rothwarf D M, Delhase M, Hayakawa M, KarinM. 1997. The IkappaB kinase complex (IKK) containstwo kinase subunits, IKKalpha and IKKbeta, necessaryfor IkappaB phosphorylation and NF-kappaBactivation. Cell, 91, 243-252

[23]Zhang Y H, Lian F Z, Zhu Y N, Xia M, Wang Q, Ling W H,Wang X D. 2010. Cyanidin-3-O-?-glucooside inhibitsLPS-induced expression of inflammatory mediatorsthrough decreasing I?B??phosphorylation in THP-1cells Inflammation Research, 59, 723-730.

Lactic Acid Reduces LPS-Induced TNF- and IL-6 mRNA Levels Through Decreasing I B Phosphorylation

Lactic Acid Reduces LPS-Induced TNF- and IL-6 mRNA Levels Through Decreasing I B Phosphorylation

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

[1]	JIANG Dai-xun, ZHANG Mei-hua, ZHANG Qian, CHEN Yi-shan, MA Wen-jing, WU Wei-peng, MU Xiang. Influence of gallic acid on porcine neutrophils phosphodiesterase 4, IL-6, TNF-α and rat arthritis model[J]. Journal of Integrative Agriculture, 2015, 14(4): 758-764.
[2]	LIU Jing-jing, LIU Xiao-ping, REN Ji-wei, ZHAO Hong-yan, YUAN Xu-feng, WANG Xiao-fen, Abdelfattah Z M Salem, CUI Zong-jun. The effects of fermentation and adsorption using lactic acid bacteria culture broth on the feed quality of rice straw[J]. Journal of Integrative Agriculture, 2015, 14(3): 503-513.
[3]	HE Yu-yong, MA Pen, LIU Xiao-lan, WANG Ren-hua , LU Wei. Effect of Different Initial pH on the Storage Characteristics and Shelf Life of Liquid Diet for Suckling and Weanling Piglets[J]. Journal of Integrative Agriculture, 2014, 13(1): 134-139.
[4]	SONG Hui, GAO Jin-feng, GAO Xiao-li, DAI Hui-ping, ZHANG Pan-an, FENG Bai-li, WANG Peng-ke. Relations Between Photosynthetic Parameters and Seed Yields of Adzuki Bean Cultivars (Vigna angularis)[J]. Journal of Integrative Agriculture, 2012, 12(9): 1453-1461.
[5]	ZHAO Xi-an, CANG Ming, GAO Xiao-yu, YANG Mei-ling, YUAN Jian-long, ZHU Bing, WANG Zhi-gang. Expression of Interleukin-6 and Interleukin-6 Receptor in Ovine Oocytes During In vitro Maturation[J]. Journal of Integrative Agriculture, 2012, 12(8): 1333-1339.
[6]	WANG Li-xia, CHENG Xu-zhen, WANG Su-hua, TIAN Jing. Analysis of anApplied Core Collection ofAdzuki Bean Germplasm by Using SSR Markers[J]. Journal of Integrative Agriculture, 2012, 12(10): 1601-1609.
[7]	LIU Jing, XUE Jiu-zhou, ZHU Zhi-ning, HU Ge, REN Xiao-ming. Lactic Acid Inhibits NF-κB Activation by Lipopolysaccharide in Rat Intestinal Mucosa Microvascular Endothelial Cells[J]. Journal of Integrative Agriculture, 2011, 10(6): 954-959.