Baicalin Induces IFN-α/β and IFN-γ Expressions in Cultured Mouse Pulmonary Microvascular Endothelial Cells

doi:10.1016/S1671-2927(00)8585

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (4): 646-654.DOI: 10.1016/S1671-2927(00)8585

Baicalin Induces IFN-α/β and IFN-γ Expressions in Cultured Mouse Pulmonary Microvascular Endothelial Cells

HU Ge, XUE Jiu-zhou, LIU Jing, ZHANG Tao, DONG Hong, DUAN Hui-qin, YANG Zuo-jun, RENXiao-ming , MU Xiang

1.Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, P.R.China
2.Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, P.R.China

收稿日期:2011-01-10 出版日期:2012-04-01 发布日期:2012-04-11
通讯作者: Correspondence MU Xiang, Tel/Fax: +86-10-80799149, E-mail: muxiang1109@sina.com; REN Xiao-ming, Tel/Fax: +86-10-80795523, E-mail: 13910224882@163.com
基金资助:
This study was supported by the National Natural Science Foundation of China (30500368), the Beijing Natural Science Foundation, China (021001), the Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality, China (PHR201008422), the Scientific Research Program of Beijing Municipal Commission of Education, China (KM201110020009), and the Beijing New Star Technology A Category Fund, China (2006A26).

Baicalin Induces IFN-α/β and IFN-γ Expressions in Cultured Mouse Pulmonary Microvascular Endothelial Cells

HU Ge, XUE Jiu-zhou, LIU Jing, ZHANG Tao, DONG Hong, DUAN Hui-qin, YANG Zuo-jun, RENXiao-ming , MU Xiang

1.Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, P.R.China
2.Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, P.R.China

Received:2011-01-10 Online:2012-04-01 Published:2012-04-11
Contact: Correspondence MU Xiang, Tel/Fax: +86-10-80799149, E-mail: muxiang1109@sina.com; REN Xiao-ming, Tel/Fax: +86-10-80795523, E-mail: 13910224882@163.com
Supported by:
This study was supported by the National Natural Science Foundation of China (30500368), the Beijing Natural Science Foundation, China (021001), the Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality, China (PHR201008422), the Scientific Research Program of Beijing Municipal Commission of Education, China (KM201110020009), and the Beijing New Star Technology A Category Fund, China (2006A26).

摘要/Abstract

摘要： We studied the effect of baicalin, an extract from Radix Scutellariae (a traditional Chinese medicine) in inducing mouse pulmonary microvascular endothelial cells (MPMVECs) to produce interferons (IFNs). MPMVECs were cultured in vitro in the presence of different concentrations of baicalin (10, 20, and 30 μg mL-1), and the cells and the culture media were harvested at various time intervals. The proteins and mRNA levels (relative to β-actin) of IFN-α/β and IFN-γ were analyzed by RT-PCR and enzyme-linked immunosorbent assay (ELISA). It was observed that baicalin substantially up-regulated the expression of IFN-α/β and IFN-γ. In all baicalin-treated groups, the relative levels of IFN-α/β and IFN-γ mRNAs peaked after 12 h of culturing, and IFN-α/β and IFN-γ proteins peaked after 24 h of culturing. These results suggest that baicalin can effectively induce the expression of IFNs in pulmonary microvascular endothelial cells, and thus potentially act as an antiviral compound. This study may provide background information for developing new antiviral drugs based on baicalin.

关键词: baicalin, pulmonary microvascular endothelial cells, interferon, mouse

Abstract: We studied the effect of baicalin, an extract from Radix Scutellariae (a traditional Chinese medicine) in inducing mouse pulmonary microvascular endothelial cells (MPMVECs) to produce interferons (IFNs). MPMVECs were cultured in vitro in the presence of different concentrations of baicalin (10, 20, and 30 μg mL-1), and the cells and the culture media were harvested at various time intervals. The proteins and mRNA levels (relative to β-actin) of IFN-α/β and IFN-γ were analyzed by RT-PCR and enzyme-linked immunosorbent assay (ELISA). It was observed that baicalin substantially up-regulated the expression of IFN-α/β and IFN-γ. In all baicalin-treated groups, the relative levels of IFN-α/β and IFN-γ mRNAs peaked after 12 h of culturing, and IFN-α/β and IFN-γ proteins peaked after 24 h of culturing. These results suggest that baicalin can effectively induce the expression of IFNs in pulmonary microvascular endothelial cells, and thus potentially act as an antiviral compound. This study may provide background information for developing new antiviral drugs based on baicalin.

Key words: baicalin, pulmonary microvascular endothelial cells, interferon, mouse

HU Ge, XUE Jiu-zhou, LIU Jing, ZHANG Tao, DONG Hong, DUAN Hui-qin, YANG Zuo-jun, RENXiao-ming , MU Xiang. Baicalin Induces IFN-α/β and IFN-γ Expressions in Cultured Mouse Pulmonary Microvascular Endothelial Cells[J]. Journal of Integrative Agriculture, 2012, 12(4): 646-654.

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Baicalin Induces IFN-α/β and IFN-γ Expressions in Cultured Mouse Pulmonary Microvascular Endothelial Cells

Baicalin Induces IFN-α/β and IFN-γ Expressions in Cultured Mouse Pulmonary Microvascular Endothelial Cells

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