Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (5): 1082-1089.DOI: 10.1016/S2095-3119(13)60313-5

• 论文 • 上一篇    下一篇

Spectrum-Effect Relationship Between High Performance Liquid Chromatography Fingerprints and Anticoccidial Activities of a Compound Chinese Medicine

 XIAO Sui, FEI Chen-zhong, ZHANG Li-fang, ZHENG Wen-li, ZHANG Ke-yu , XUE Fei-qun   

  1. Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R.China
  • 收稿日期:2012-12-27 出版日期:2014-05-01 发布日期:2014-05-06
  • 通讯作者: XUE Fei-qun, Tel: +86-21-34293460, Fax: +86-21-34293396, E-mail: xuefeiqun@yahoo.cn, feiqun@gmail.com
  • 基金资助:

    The authors would like to acknowledge the Key Laboratory of Animal Parasitology of the Ministry of Agriculture for providing the laboratory equipments used in this study. This work was funded by the National Key Technology R&D Program, China (2008 BADB4B05-02).

Spectrum-Effect Relationship Between High Performance Liquid Chromatography Fingerprints and Anticoccidial Activities of a Compound Chinese Medicine

 XIAO Sui, FEI Chen-zhong, ZHANG Li-fang, ZHENG Wen-li, ZHANG Ke-yu , XUE Fei-qun   

  1. Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R.China
  • Received:2012-12-27 Online:2014-05-01 Published:2014-05-06
  • Contact: XUE Fei-qun, Tel: +86-21-34293460, Fax: +86-21-34293396, E-mail: xuefeiqun@yahoo.cn, feiqun@gmail.com
  • Supported by:

    The authors would like to acknowledge the Key Laboratory of Animal Parasitology of the Ministry of Agriculture for providing the laboratory equipments used in this study. This work was funded by the National Key Technology R&D Program, China (2008 BADB4B05-02).

PDF

摘要： Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures. The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography (HPLC) fingerprints and anticoccidial activities. Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine. In addition, pharmacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time. We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample. Furthermore, effect of this medicine was related with the main active constituents, along with the origin and the harvesting time.

关键词: herb medicine , fingerprint , anticoccidial index , spectrum-effect relationship

Abstract: Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures. The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography (HPLC) fingerprints and anticoccidial activities. Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine. In addition, pharmacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time. We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample. Furthermore, effect of this medicine was related with the main active constituents, along with the origin and the harvesting time.

Key words: herb medicine , fingerprint , anticoccidial index , spectrum-effect relationship