Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (5): 1090-1098.DOI: 10.1016/S2095-3119(13)60740-6

• 论文 • 上一篇    下一篇

Differential Responses of Nitrifier and Denitrifier to Dicyandiamide in Short- and Long-Term Intensive Vegetable Cultivation Soils

 LIU Yi, YANG Yang,  QIN Hong-ling, ZHU Yi-jun , WEI Wen-xue   

  1. 1、Key Laboratory of Agro-Ecological Processes in Subtropical Regions and Taoyuan Station of Agro-Ecology Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, P.R.China
    2、Tianjin Land & Resources & Housing Management Research Centre, Tianjin 300041, P.R.China
  • 收稿日期:2013-01-10 出版日期:2014-05-01 发布日期:2014-05-06
  • 通讯作者: WEI Wen-xue, Tel: +86-731-84615210, E-mail: wenxuewei@isa.ac.cn
  • 作者简介:LIU Yi, Mobile: 13467585846, E-mail: liuyi@isa.ac.cn;
  • 基金资助:

    This work was financially supported by the National Natural Science Foundation of China (41071181, 41090282) and the Chinese Academy of Sciences (KZCX2-YW-T07).

Differential Responses of Nitrifier and Denitrifier to Dicyandiamide in Short- and Long-Term Intensive Vegetable Cultivation Soils

 LIU Yi, YANG Yang,  QIN Hong-ling, ZHU Yi-jun , WEI Wen-xue   

  1. 1、Key Laboratory of Agro-Ecological Processes in Subtropical Regions and Taoyuan Station of Agro-Ecology Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, P.R.China
    2、Tianjin Land & Resources & Housing Management Research Centre, Tianjin 300041, P.R.China
  • Received:2013-01-10 Online:2014-05-01 Published:2014-05-06
  • Contact: WEI Wen-xue, Tel: +86-731-84615210, E-mail: wenxuewei@isa.ac.cn
  • About author:LIU Yi, Mobile: 13467585846, E-mail: liuyi@isa.ac.cn;
  • Supported by:

    This work was financially supported by the National Natural Science Foundation of China (41071181, 41090282) and the Chinese Academy of Sciences (KZCX2-YW-T07).

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摘要： Nitrification inhibitors, such as dicyandiamide (DCD), have been shown to decrease leaching from urea- and ammonium- based fertilizers in agricultural soils. The effect of nitrification inhibitors on nitrifier and denitrifier in short- and long-term intensive vegetable cultivation soils was poorly understood. In this study, the pot trial was conducted to investigate the differential responses of nitrifier (amoA-containing bacteria) and denitrifier (nirK-containing bacteria) to DCD in short-(soil S) and long-term (soil L) intensive vegetable cultivation soils. Quantitative polymerase chain reaction (qPCR) and terminal restriction fragment length polymorphism (T-RFLP) were employed to detect the abundance and composition of amoA- and nirK-containing communities. The results indicated that application of DCD led to a consistently higher NH4 +-N concentration during the whole incubation in soil L, while it was quickly decreased in soil S after 21 days. Furthermore, DCD induced more severe decrease of the abundance of amoA-containing bacteria in soil L than in soil S. However, the abundance of the nirKcontaining community was not significantly affected by DCD in both soils. Long-term vegetable cultivation resulted in a super-dominant amoA-containing bacteria group and less divergence in soil L compared with soil S, and DCD did not cause obvious shifts of the composition of ammonia-oxidising bacteria (AOB). On the contrary, both amoA- and nirK-containing bacterial compositions were influenced by DCD in soil S. The results suggested that long-term intensive vegetable cultivation with heavy nitrogen fertilization resulted in significant shifts of AOB community, and this community was sensitive to DCD, but denitrifiers were not clearly affected by DCD.

关键词: DCD , amoA , nirK , vegetable soil

Abstract: Nitrification inhibitors, such as dicyandiamide (DCD), have been shown to decrease leaching from urea- and ammonium- based fertilizers in agricultural soils. The effect of nitrification inhibitors on nitrifier and denitrifier in short- and long-term intensive vegetable cultivation soils was poorly understood. In this study, the pot trial was conducted to investigate the differential responses of nitrifier (amoA-containing bacteria) and denitrifier (nirK-containing bacteria) to DCD in short-(soil S) and long-term (soil L) intensive vegetable cultivation soils. Quantitative polymerase chain reaction (qPCR) and terminal restriction fragment length polymorphism (T-RFLP) were employed to detect the abundance and composition of amoA- and nirK-containing communities. The results indicated that application of DCD led to a consistently higher NH4 +-N concentration during the whole incubation in soil L, while it was quickly decreased in soil S after 21 days. Furthermore, DCD induced more severe decrease of the abundance of amoA-containing bacteria in soil L than in soil S. However, the abundance of the nirKcontaining community was not significantly affected by DCD in both soils. Long-term vegetable cultivation resulted in a super-dominant amoA-containing bacteria group and less divergence in soil L compared with soil S, and DCD did not cause obvious shifts of the composition of ammonia-oxidising bacteria (AOB). On the contrary, both amoA- and nirK-containing bacterial compositions were influenced by DCD in soil S. The results suggested that long-term intensive vegetable cultivation with heavy nitrogen fertilization resulted in significant shifts of AOB community, and this community was sensitive to DCD, but denitrifiers were not clearly affected by DCD.

Key words: DCD , amoA , nirK , vegetable soil