The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana

doi:10.1016/S2095-3119(13)60248-8

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (3): 474-482.DOI: 10.1016/S2095-3119(13)60248-8

The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana

JIANG Wei-yu, GENG Li-li, DAI Ping-li, LANG Zhi-hong, SHU Chang-long, LIN Yi, ZHOU Ting, SONG Fu-ping , ZHANG Jie

1.State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2.College of Chemical Engineering, Huaqiao University, Xiamen 361021, P.R.China
3.Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture/Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, P.R.China
4.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

收稿日期:2012-02-01 出版日期:2013-03-01 发布日期:2013-03-10
通讯作者: Correspondence ZHANG Jie, Tel: +86-10-62815921, Fax: +86-10-62812632, E-mail: jzhang@ippcaas.cn
基金资助:
This work was supported by the National Basic Research Program of China (2007CB109203 and 2009CB118902).

The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana

JIANG Wei-yu, GENG Li-li, DAI Ping-li, LANG Zhi-hong, SHU Chang-long, LIN Yi, ZHOU Ting, SONG Fu-ping , ZHANG Jie

1.State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
2.College of Chemical Engineering, Huaqiao University, Xiamen 361021, P.R.China
3.Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture/Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, P.R.China
4.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2012-02-01 Online:2013-03-01 Published:2013-03-10
Contact: Correspondence ZHANG Jie, Tel: +86-10-62815921, Fax: +86-10-62812632, E-mail: jzhang@ippcaas.cn
Supported by:
This work was supported by the National Basic Research Program of China (2007CB109203 and 2009CB118902).

摘要/Abstract

摘要： Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the Chinese honeybee, Apis cerana cerana, was assessed. Honeybees were fed with Bt-transgenic maize pollen, non-transgenic near isoline pollen, linear cry1Ah gene (800 ng mL-1) and supercoiled plasmid DNA (800 ng mL-1) under laboratory conditions. The DGGE profile showed that the number of DGGE bands varied from 10.7 to 14.7 per sample, and the Shannon’s index ranged from 0.85 to 1.00. The similarity calculated by PAST was mostly above 92%, indicating no obvious changes among treatments or within replicates. 14 bacterial strains affiliated with Alphaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were isolated and characterized on media under aerobic and anaerobic conditions. These results demonstrated that transgenic cry1Ah maize pollen did not induce significant changes of the honeybee gut bacterial community composition under laboratory conditions.

关键词: Bacillus thuringiensis, intestinal bacteria, horizontal gene transfer, transgenic maize, Apis cerana, biosafety

Abstract: Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the Chinese honeybee, Apis cerana cerana, was assessed. Honeybees were fed with Bt-transgenic maize pollen, non-transgenic near isoline pollen, linear cry1Ah gene (800 ng mL-1) and supercoiled plasmid DNA (800 ng mL-1) under laboratory conditions. The DGGE profile showed that the number of DGGE bands varied from 10.7 to 14.7 per sample, and the Shannon’s index ranged from 0.85 to 1.00. The similarity calculated by PAST was mostly above 92%, indicating no obvious changes among treatments or within replicates. 14 bacterial strains affiliated with Alphaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were isolated and characterized on media under aerobic and anaerobic conditions. These results demonstrated that transgenic cry1Ah maize pollen did not induce significant changes of the honeybee gut bacterial community composition under laboratory conditions.

JIANG Wei-yu, GENG Li-li, DAI Ping-li, LANG Zhi-hong, SHU Chang-long, LIN Yi, ZHOU Ting, SONG Fu-ping , ZHANG Jie. The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana[J]. Journal of Integrative Agriculture, 2013, 12(3): 474-482.

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The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana

The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana

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