Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (12): 1968-1976.DOI: 10.1016/S1671-2927(11)60198-3

• 论文 • 上一篇    下一篇

Pharmacokinetics of Mequindox and Its Metabolites in Swine

 LIU Yi-ming, LIU Ying-chun, DING Huan-zhong, FANG Bing-hu, YANG Fan, SHAN Qi , ZENGZhen-ling   

  1. 1. Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510640, P.R.China
    2. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200232, P.R.China
  • 收稿日期:2010-10-22 出版日期:2011-12-01 发布日期:2011-12-19
  • 通讯作者: Correspondence ZENG Zhen-ling, Mobile: 13609768425, E-mail: zlzeng@scau.edu.cn
  • 作者简介:LIU Yi-ming, Mobile: 13718937129, E-mail: liuyiming@caas.net.cn
  • 基金资助:

    This study was supported by the National Basic Research Program of China (973 Program, 2009CB118805) and the National Key Technology Research and Development Program of China during the 10th Five-Year Plan Period (2009BADB7B05-03).

Pharmacokinetics of Mequindox and Its Metabolites in Swine

 LIU Yi-ming, LIU Ying-chun, DING Huan-zhong, FANG Bing-hu, YANG Fan, SHAN Qi , ZENGZhen-ling   

  1. 1. Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510640, P.R.China
    2. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200232, P.R.China
  • Received:2010-10-22 Online:2011-12-01 Published:2011-12-19
  • Contact: Correspondence ZENG Zhen-ling, Mobile: 13609768425, E-mail: zlzeng@scau.edu.cn
  • About author:LIU Yi-ming, Mobile: 13718937129, E-mail: liuyiming@caas.net.cn
  • Supported by:

    This study was supported by the National Basic Research Program of China (973 Program, 2009CB118805) and the National Key Technology Research and Development Program of China during the 10th Five-Year Plan Period (2009BADB7B05-03).

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摘要： The present study was carried out to investigate the pharmacokinetics of mequindox (MEQ), a new synthetic quinoxaline 1,4-dioxide derivative and its two main metabolites M1 [2-isoethanol mequinoox], M2 [2-isoethanol 1-desoxymequindox] in healthy swine. MEQ (10 mg kg-1 body weight) was administered to nine healthy cross-bread swine via oral, intramuscular, and intravenous routes in a randomized 3×3 crossover design with a 1-wk washout period. A sensitive high-performance liquid chromatography (HPLC) method was used for the determination of plasma concentrations of MEQ and its metabolites M1 and M2. Plasma concentration versus time profiles of MEQ and its metabolites, M1 and M2, were analyzed by noncompartmental analysis using WinNonlin 5.2 software. The mean maximum concentrations (Cmax) of M1 and M2 after intravenous administration of MEQ were (5.27±1.59) μg mL-1 at 1.78 h and (1.01±0.29) μg mL-1 at 0.92 h, respectively. The mean maximum concentrations (Cmax) of MEQ, M1, and M2 were found to be (6.96±3.23), (6.61±1.56), and (0.78 ±0.25) μg mL-1, respectively at 0.15, 1.61, and 1.30 h after intramuscular administration of MEQ, respectively and (0.75±0.45), (6.90±1.52), and (0.62±0.21) μg mL-1, respectively at 0.40, 1.57, and 2.00 h, respectively after oral administration of MEQ. The apparent elimination half-lives (t1/2) of MEQ, M1, and M2 were (0.84±0.35), (7.57±3.93), and (9.56±6.00) h, respectively after intravenous administration of MEQ; (0.50±0.25), (6.30±3.00), and (5.94±2.54) h, respectively after intramuscular administration of MEQ; and (1.64±1.17), (5.59±1.93), and (16.25±10.27) h , respectively after oral administration of MEQ. The mean areas under the plasma concentration-time curve (AUC0- ) of MEQ, M1, and M2 were (4.88±1.54), (36.93±17.50), and (5.16±1.94) μg h mL-1, respectively after intravenous administration of MEQ; (4.18±0.76), (48.25±20.82), and (4.88±2.21) μg h mL-1 , respectively after intramuscular administration of MEQ; and (1.01±0.40), (48.83±20.71), and (5.54±2.23) μg h mL-1, respectively after oral administration of MEQ. MEQ was rapidly absorbed and metabolized in swine after oral, intramuscular, and intravenous administration. Further studies are required to investigate the double-peak phenomenon observed in the plasma concentration-time profile after oral administration and the pharmacokinetics of other metabolites of MEQ.

关键词: mequindox, metabolites, pharmacokinetics, HPLC, swine

Abstract: The present study was carried out to investigate the pharmacokinetics of mequindox (MEQ), a new synthetic quinoxaline 1,4-dioxide derivative and its two main metabolites M1 [2-isoethanol mequinoox], M2 [2-isoethanol 1-desoxymequindox] in healthy swine. MEQ (10 mg kg-1 body weight) was administered to nine healthy cross-bread swine via oral, intramuscular, and intravenous routes in a randomized 3×3 crossover design with a 1-wk washout period. A sensitive high-performance liquid chromatography (HPLC) method was used for the determination of plasma concentrations of MEQ and its metabolites M1 and M2. Plasma concentration versus time profiles of MEQ and its metabolites, M1 and M2, were analyzed by noncompartmental analysis using WinNonlin 5.2 software. The mean maximum concentrations (Cmax) of M1 and M2 after intravenous administration of MEQ were (5.27±1.59) μg mL-1 at 1.78 h and (1.01±0.29) μg mL-1 at 0.92 h, respectively. The mean maximum concentrations (Cmax) of MEQ, M1, and M2 were found to be (6.96±3.23), (6.61±1.56), and (0.78 ±0.25) μg mL-1, respectively at 0.15, 1.61, and 1.30 h after intramuscular administration of MEQ, respectively and (0.75±0.45), (6.90±1.52), and (0.62±0.21) μg mL-1, respectively at 0.40, 1.57, and 2.00 h, respectively after oral administration of MEQ. The apparent elimination half-lives (t1/2) of MEQ, M1, and M2 were (0.84±0.35), (7.57±3.93), and (9.56±6.00) h, respectively after intravenous administration of MEQ; (0.50±0.25), (6.30±3.00), and (5.94±2.54) h, respectively after intramuscular administration of MEQ; and (1.64±1.17), (5.59±1.93), and (16.25±10.27) h , respectively after oral administration of MEQ. The mean areas under the plasma concentration-time curve (AUC0- ) of MEQ, M1, and M2 were (4.88±1.54), (36.93±17.50), and (5.16±1.94) μg h mL-1, respectively after intravenous administration of MEQ; (4.18±0.76), (48.25±20.82), and (4.88±2.21) μg h mL-1 , respectively after intramuscular administration of MEQ; and (1.01±0.40), (48.83±20.71), and (5.54±2.23) μg h mL-1, respectively after oral administration of MEQ. MEQ was rapidly absorbed and metabolized in swine after oral, intramuscular, and intravenous administration. Further studies are required to investigate the double-peak phenomenon observed in the plasma concentration-time profile after oral administration and the pharmacokinetics of other metabolites of MEQ.

Key words: mequindox, metabolites, pharmacokinetics, HPLC, swine