Tissue distribution of deoxynivalenol in piglets following intravenous administration

doi:10.1016/S2095-3119(14)60942-4

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (10): 2058-2064.DOI: 10.1016/S2095-3119(14)60942-4

Tissue distribution of deoxynivalenol in piglets following intravenous administration

DENG Xian-bai, DIN Huan-zhong, HUANG Xian-hui, MA Yong-jiang, FAN Xiao-long, YAN Hai-kuo, LU Pei-cheng, LI Wei-cheng, ZENG Zhen-ling

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China

收稿日期:2014-09-23 出版日期:2015-10-09 发布日期:2015-10-12
通讯作者: ZENG Zhen-ling, Mobile: +86-13609768425,Fax: +86-20-85280234, E-mail: zlzeng@scau.edu.cn
作者简介:DENG Xian-bai, E-mail: xbdeng@scau.edu.cn;
基金资助:
Financial support for this work was provided by the National Basic Research Program of China (2009CB118805) and the Centre for Veterinary of Drug Residues, College of Veterinary Medicine, South China Agricultural University.

Tissue distribution of deoxynivalenol in piglets following intravenous administration

DENG Xian-bai, DIN Huan-zhong, HUANG Xian-hui, MA Yong-jiang, FAN Xiao-long, YAN Hai-kuo, LU Pei-cheng, LI Wei-cheng, ZENG Zhen-ling

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China

Received:2014-09-23 Online:2015-10-09 Published:2015-10-12
Contact: ZENG Zhen-ling, Mobile: +86-13609768425,Fax: +86-20-85280234, E-mail: zlzeng@scau.edu.cn
About author:DENG Xian-bai, E-mail: xbdeng@scau.edu.cn;
Supported by:
Financial support for this work was provided by the National Basic Research Program of China (2009CB118805) and the Centre for Veterinary of Drug Residues, College of Veterinary Medicine, South China Agricultural University.

摘要/Abstract

摘要： Contamination of deoxynivalenol (DON) in grains is common worldwide and pigs are particularly susceptible to this mycotoxin. The distribution of DON in porcine tissues following intravenous administration was investigated in this study. Fifteen pigs were randomly divided into three groups. Animals in groups A and B were administrated with DON at the dose of 250 and 750 μg kg–1 body weight, respectively, while group C served as blank control. Plasma, bile and 27 tissues were collected at 30 min post-administration. DON concentrations in all samples were tested using high-performance liquid chromatography- tandem mass spectrometry (HPLC-MS/MS). To observe the distribution of DON in tissues, these samples were further subjected to the immunohistochemical analyses. Totally, the bile and 13 tissues were sampled for DON-based detection, including kidney, mesenteric lymph nodes, muscle, stomach, jejunum, colon, plasma, spleen, rectum, cecum, liver, ileum, and duodenum. No significant difference was observed for the concentrations of DON in duodenum, ileum and liver samples between groups A and B; while the DON concentrations in cecum and rectum of group B were significantly higher (P-value <0.05) than those in group A. In addition, the DON concentrations in stomach, jejunum, colon, mesenteric lymph nodes, muscle, kidney, spleen, bile, and plasma of group B were remarkably higher than those of group A (P-value<0.01). Levels of DON in other 14 tissues including medulla oblongata, midbrain, diencephalon, pons, tip and tongue body, tongue, soft palate, tonsils, pharyngeal mucosa, oral buccal mucosa, thymus, thyroid, esophagus and adrenal gland were all below the limit of detection. The results of immunohistochemistry showed that 11 tissue samples (medullaoblongata, tonsil, adrenal medulla, thyroid gland, thyroid, stomach, duodenum, jejunum, kidney, spleen, and mesenteric lymph nodes) were positive and DON was mainly distributed around blood vessels in these tissues. Therefore, we believed that concentrations of DON in tissues differ when pigs are in exposure to various dosages and DON causes lesions in many pig tissues.

关键词: deoxynivalenol , tissue distribution , immunohistochemical staining , HPLC-MS/MS , swine

Abstract: Contamination of deoxynivalenol (DON) in grains is common worldwide and pigs are particularly susceptible to this mycotoxin. The distribution of DON in porcine tissues following intravenous administration was investigated in this study. Fifteen pigs were randomly divided into three groups. Animals in groups A and B were administrated with DON at the dose of 250 and 750 μg kg–1 body weight, respectively, while group C served as blank control. Plasma, bile and 27 tissues were collected at 30 min post-administration. DON concentrations in all samples were tested using high-performance liquid chromatography- tandem mass spectrometry (HPLC-MS/MS). To observe the distribution of DON in tissues, these samples were further subjected to the immunohistochemical analyses. Totally, the bile and 13 tissues were sampled for DON-based detection, including kidney, mesenteric lymph nodes, muscle, stomach, jejunum, colon, plasma, spleen, rectum, cecum, liver, ileum, and duodenum. No significant difference was observed for the concentrations of DON in duodenum, ileum and liver samples between groups A and B; while the DON concentrations in cecum and rectum of group B were significantly higher (P-value <0.05) than those in group A. In addition, the DON concentrations in stomach, jejunum, colon, mesenteric lymph nodes, muscle, kidney, spleen, bile, and plasma of group B were remarkably higher than those of group A (P-value<0.01). Levels of DON in other 14 tissues including medulla oblongata, midbrain, diencephalon, pons, tip and tongue body, tongue, soft palate, tonsils, pharyngeal mucosa, oral buccal mucosa, thymus, thyroid, esophagus and adrenal gland were all below the limit of detection. The results of immunohistochemistry showed that 11 tissue samples (medullaoblongata, tonsil, adrenal medulla, thyroid gland, thyroid, stomach, duodenum, jejunum, kidney, spleen, and mesenteric lymph nodes) were positive and DON was mainly distributed around blood vessels in these tissues. Therefore, we believed that concentrations of DON in tissues differ when pigs are in exposure to various dosages and DON causes lesions in many pig tissues.

Key words: deoxynivalenol , tissue distribution , immunohistochemical staining , HPLC-MS/MS , swine

DENG Xian-bai, DIN Huan-zhong, HUANG Xian-hui, MA Yong-jiang, FAN Xiao-long, YAN Hai-kuo, LU Pei-cheng, LI Wei-cheng, ZENG Zhen-ling. Tissue distribution of deoxynivalenol in piglets following intravenous administration[J]. Journal of Integrative Agriculture, 2015, 14(10): 2058-2064.

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Tissue distribution of deoxynivalenol in piglets following intravenous administration

Tissue distribution of deoxynivalenol in piglets following intravenous administration

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