Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (1): 80-87.DOI: 10.1016/S2095-3119(14)60905-9

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Secondary metabolites of rice sheath blight pathogen Rhizoctonia solani Kühn and their biological activities

 XU Liang, WANG Xiao-han, LUO Rui-ya, LU Shi-qiong, GUO Ze-jian, WANG Ming-an, LIU Yang, ZHOU Li-gang   

  1. 1、Key Laboratory of Plant Pathology, Ministry of Agriculture/Department of Plant Pathology, College of Agronomy and
    Biotechnology, China Agricultural University, Beijing 100193, P.R.China
    2、Institute of Agro-Products Processing and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
  • 收稿日期:2014-02-10 出版日期:2015-01-01 发布日期:2015-01-08
  • 通讯作者: ZHOU Li-gang, Tel: +86-10-62731199, Fax: +86-10-62731062,E-mail: lgzhou@cau.edu.cn
  • 作者简介:XU Liang, E-mail: 1baf2006@163.com
  • 基金资助:

    This work was co-financed by the grants from the National Basic Research Program of China (2013GB127800) and the National Natural Science Foundation of China (31271996).

Secondary metabolites of rice sheath blight pathogen Rhizoctonia solani Kühn and their biological activities

 XU Liang, WANG Xiao-han, LUO Rui-ya, LU Shi-qiong, GUO Ze-jian, WANG Ming-an, LIU Yang, ZHOU Li-gang   

  1. 1、Key Laboratory of Plant Pathology, Ministry of Agriculture/Department of Plant Pathology, College of Agronomy and
    Biotechnology, China Agricultural University, Beijing 100193, P.R.China
    2、Institute of Agro-Products Processing and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
  • Received:2014-02-10 Online:2015-01-01 Published:2015-01-08
  • Contact: ZHOU Li-gang, Tel: +86-10-62731199, Fax: +86-10-62731062,E-mail: lgzhou@cau.edu.cn
  • About author:XU Liang, E-mail: 1baf2006@163.com
  • Supported by:

    This work was co-financed by the grants from the National Basic Research Program of China (2013GB127800) and the National Natural Science Foundation of China (31271996).

摘要: Eight compounds were isolated from the fermentation cultures of rice sheath blight pathogen Rhizoctonia solani Kühn. They were identified as ergosterol (1), 6β-hydroxysitostenone (2), sitostenone (3), m-hydroxyphenylacetic acid (4), methyl m-hydroxyphenylacetate (5), m-hydroxymethylphenyl pentanoate (6), (Z)-3-methylpent-2-en-1,5-dioic acid (7) and 3-methoxyfuran-2-carboxylic acid (8) by means of physicochemical and spectroscopic analysis. Among them, 2, 3, 5–8 were isolated from R. solani for the first time. All the compounds were evaluated for their biological activities. 4–6 and 8 showed their inhibitory activities on the radical and germ elongation of rice seeds. 1, 4 and 7 showed moderate antibacterial activity to some bacteria. 4, 7 and 8 exhibited weak inhibitory activities on spore germination of Magnaporthe oryzae. 8 showed moderate antioxidant activity with the 1,1-diphenyl-2-picryhydrazyl (DPPH) and β-carotene-linoleic acid assays. This is the first time to reveal compounds 5, 6 and 8 from rice sheath blight pathogen R. solani to have in vitro phytotoxic activity.

关键词: rice sheath blight , Rhizoctonia solani Kü, hn , secondary metabolites , bioactivities , in vitro phytotoxic activity

Abstract: Eight compounds were isolated from the fermentation cultures of rice sheath blight pathogen Rhizoctonia solani Kühn. They were identified as ergosterol (1), 6β-hydroxysitostenone (2), sitostenone (3), m-hydroxyphenylacetic acid (4), methyl m-hydroxyphenylacetate (5), m-hydroxymethylphenyl pentanoate (6), (Z)-3-methylpent-2-en-1,5-dioic acid (7) and 3-methoxyfuran-2-carboxylic acid (8) by means of physicochemical and spectroscopic analysis. Among them, 2, 3, 5–8 were isolated from R. solani for the first time. All the compounds were evaluated for their biological activities. 4–6 and 8 showed their inhibitory activities on the radical and germ elongation of rice seeds. 1, 4 and 7 showed moderate antibacterial activity to some bacteria. 4, 7 and 8 exhibited weak inhibitory activities on spore germination of Magnaporthe oryzae. 8 showed moderate antioxidant activity with the 1,1-diphenyl-2-picryhydrazyl (DPPH) and β-carotene-linoleic acid assays. This is the first time to reveal compounds 5, 6 and 8 from rice sheath blight pathogen R. solani to have in vitro phytotoxic activity.

Key words: rice sheath blight , Rhizoctonia solani Kühn , secondary metabolites , bioactivities , in vitro phytotoxic activity