Effect of Semen vaccariae and Taraxacumogono on CellAdhesion of Bovine Mammary Epithelial Cells

doi:10.1016/S1671-2927(00)8742

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (12): 2043-2050.DOI: 10.1016/S1671-2927(00)8742

Effect of Semen vaccariae and Taraxacumogono on CellAdhesion of Bovine Mammary Epithelial Cells

TONG Jin-jin, LI Ye, LIU Rong, GAO Xue-jun , LI Qing-zhang

Key Laboratory of Dairy Science, Ministry of Education/College of Life Science, Northeast Agricultural University, Harbin 150030, P.R. China

收稿日期:2011-06-27 出版日期:2012-12-01 发布日期:2012-12-18
通讯作者: Correspondence LI Qing-zhang, E-mail: qingzhangli@hotmail.com
作者简介:TONG Jin-jin, E-mail: tongjinjin0451@163.com
基金资助:
This study was financially supported by the National Basic Research Program of China (2011CB100804).

Effect of Semen vaccariae and Taraxacumogono on CellAdhesion of Bovine Mammary Epithelial Cells

TONG Jin-jin, LI Ye, LIU Rong, GAO Xue-jun , LI Qing-zhang

Key Laboratory of Dairy Science, Ministry of Education/College of Life Science, Northeast Agricultural University, Harbin 150030, P.R. China

Received:2011-06-27 Online:2012-12-01 Published:2012-12-18
Contact: Correspondence LI Qing-zhang, E-mail: qingzhangli@hotmail.com
About author:TONG Jin-jin, E-mail: tongjinjin0451@163.com
Supported by:
This study was financially supported by the National Basic Research Program of China (2011CB100804).

摘要/Abstract

摘要： The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and b-catenin on the proliferation role and secretion function of bovine mammary epithelial cells cultured in vitro. Firstly, the epithelial character of bovine mammary epithelial cells was authenticated using immunofluorescence, then the cell grow curve was observed and investigated after S. vaccariae and T. mogono treatment. On the effect of S. vaccariae and T. mogono, cell adhesion molecules E-cadherin, b-catenin and CycinD1 mRNA and protein were detected by qRT-PCR and Western blotting, respectively. The results showed that the cellular keratin 18 expressed positively and proliferated vigorously after S. vaccariae and T. mogono treament. The mRNA and protein levels of E-cadherin and CycinD1 were remarkably higher (P<0.05) in 36 h after S. vaccariae and T. mogono treatment. The cell proliferation at 36 h was increased significantly (P<0.05). In conclusion, S. vaccariae and T. mogono have a positive impact on the cell proliferation and an effect on the adhesion molecules E-cadherin, b-catenin and CycinD1 in the Wnt signaling pathway.

关键词: E-cadherin, b-catenin, Semen vaccariae, Taraxacu mogono, bovine, mammary epithelial cells

Abstract: The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and b-catenin on the proliferation role and secretion function of bovine mammary epithelial cells cultured in vitro. Firstly, the epithelial character of bovine mammary epithelial cells was authenticated using immunofluorescence, then the cell grow curve was observed and investigated after S. vaccariae and T. mogono treatment. On the effect of S. vaccariae and T. mogono, cell adhesion molecules E-cadherin, b-catenin and CycinD1 mRNA and protein were detected by qRT-PCR and Western blotting, respectively. The results showed that the cellular keratin 18 expressed positively and proliferated vigorously after S. vaccariae and T. mogono treament. The mRNA and protein levels of E-cadherin and CycinD1 were remarkably higher (P<0.05) in 36 h after S. vaccariae and T. mogono treatment. The cell proliferation at 36 h was increased significantly (P<0.05). In conclusion, S. vaccariae and T. mogono have a positive impact on the cell proliferation and an effect on the adhesion molecules E-cadherin, b-catenin and CycinD1 in the Wnt signaling pathway.

Key words: E-cadherin, b-catenin, Semen vaccariae, Taraxacu mogono, bovine, mammary epithelial cells

TONG Jin-jin, LI Ye, LIU Rong, GAO Xue-jun , LI Qing-zhang. Effect of Semen vaccariae and Taraxacumogono on CellAdhesion of Bovine Mammary Epithelial Cells[J]. Journal of Integrative Agriculture, 2012, 12(12): 2043-2050.

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Effect of Semen vaccariae and Taraxacumogono on CellAdhesion of Bovine Mammary Epithelial Cells

Effect of Semen vaccariae and Taraxacumogono on CellAdhesion of Bovine Mammary Epithelial Cells

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