UsingPhospholipid FattyAcid Technique toAnalysis theRhizosphere Specific Microbial Community of Seven Hybrid Rice Cultivars

doi:10.1016/S1671-2927(00)8716

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (11): 1817-1827.DOI: 10.1016/S1671-2927(00)8716

UsingPhospholipid FattyAcid Technique toAnalysis theRhizosphere Specific Microbial Community of Seven Hybrid Rice Cultivars

ZHU Yu-jing, HU Gui-ping, LIU Bo, XIE Hua-an, ZHENG Xue-fang , ZHANG Jian-fu

1.Fujian Academy of Agricultural Sciences, Fuzhou 350003, P.R.China
2.Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China

收稿日期:2011-11-14 出版日期:2012-11-01 发布日期:2012-11-24
通讯作者: Correspondence LIU Bo, Tel/Fax: +86-591-87864601, E-mail: liubofaas@163.com
作者简介:ZHU Yu-jing, Mobile: 13960770801, Tel: +86-591-87882571, E-mail: zyjingfz@163.com; HU Gui-ping, E-mail: hugp_2007@163.com;
基金资助:
This work was supported by the National Basic Research Program of China (2011CB111607) and the Fujian Funds for Distinguished Young Scientists, China (2009J06010).

UsingPhospholipid FattyAcid Technique toAnalysis theRhizosphere Specific Microbial Community of Seven Hybrid Rice Cultivars

ZHU Yu-jing, HU Gui-ping, LIU Bo, XIE Hua-an, ZHENG Xue-fang , ZHANG Jian-fu

1.Fujian Academy of Agricultural Sciences, Fuzhou 350003, P.R.China
2.Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China

Received:2011-11-14 Online:2012-11-01 Published:2012-11-24
Contact: Correspondence LIU Bo, Tel/Fax: +86-591-87864601, E-mail: liubofaas@163.com
About author:ZHU Yu-jing, Mobile: 13960770801, Tel: +86-591-87882571, E-mail: zyjingfz@163.com; HU Gui-ping, E-mail: hugp_2007@163.com;
Supported by:
This work was supported by the National Basic Research Program of China (2011CB111607) and the Fujian Funds for Distinguished Young Scientists, China (2009J06010).

摘要/Abstract

摘要： To analyze the intrinsic relationship between rhizosphere microbial community structure and variety of rice, the microbial community structures in rhizosphere of different hybrid rice cultivars were determined with phospholipid fatty acids (PLFA) analysis. Three series of new-breeding hybrid rice cultivars in China were tested in the experiment, IIyouming 86 (II-32A/Minghui 86), IIyouhang 1 (II-32A/Hang 1), and IIyouhang 2 (II-32A/Hang 2) with II-32A as female parent, XinyouHK02 (XinA/HK02) and YiyouHK02 (YXA/HK02) with HK02 as male parent, Chuanyou 167 (ChuanxiangA/MR167) and 44you167 (Hunan44A/MR167) with MR167 as male parent. The results showed that the microbial community in rhizosphere of the hybrid rice comprised bacteria, fungi, actinomycetes, and protozoa, according to the 40 PLFA biomarkers detected. Bacteria were more abundant than fungi and actinomycetes in rhizosphere of the hybrid rice tested. Both sulfate-reducing and methane-oxidizing bacteria were found to exist in the hybrid rice rhizosphere. It was also found that the characteristics of PLFA biomarkers had correlation with the biological traits of rice. The cluster analysis suggested that microbial community structure and activity in rhizosphere were associated with genetic background of the rice cultivar.

关键词: hybrid rice , rhizosphere microorganism , phospholipid fatty acid , diversity

Abstract: To analyze the intrinsic relationship between rhizosphere microbial community structure and variety of rice, the microbial community structures in rhizosphere of different hybrid rice cultivars were determined with phospholipid fatty acids (PLFA) analysis. Three series of new-breeding hybrid rice cultivars in China were tested in the experiment, IIyouming 86 (II-32A/Minghui 86), IIyouhang 1 (II-32A/Hang 1), and IIyouhang 2 (II-32A/Hang 2) with II-32A as female parent, XinyouHK02 (XinA/HK02) and YiyouHK02 (YXA/HK02) with HK02 as male parent, Chuanyou 167 (ChuanxiangA/MR167) and 44you167 (Hunan44A/MR167) with MR167 as male parent. The results showed that the microbial community in rhizosphere of the hybrid rice comprised bacteria, fungi, actinomycetes, and protozoa, according to the 40 PLFA biomarkers detected. Bacteria were more abundant than fungi and actinomycetes in rhizosphere of the hybrid rice tested. Both sulfate-reducing and methane-oxidizing bacteria were found to exist in the hybrid rice rhizosphere. It was also found that the characteristics of PLFA biomarkers had correlation with the biological traits of rice. The cluster analysis suggested that microbial community structure and activity in rhizosphere were associated with genetic background of the rice cultivar.

Key words: hybrid rice , rhizosphere microorganism , phospholipid fatty acid , diversity

ZHUYu-jing, HU Gui-ping, LIU Bo, XIE Hua-an, ZHENGXue-fang , ZHANG Jian-fu. UsingPhospholipid FattyAcid Technique toAnalysis theRhizosphere Specific Microbial Community of Seven Hybrid Rice Cultivars[J]. Journal of Integrative Agriculture, 2012, 12(11): 1817-1827.

ZHUYu-jing , HU Gui-ping, LIU Bo, XIE Hua-an, ZHENGXue-fang , ZHANG Jian-fu. UsingPhospholipid FattyAcid Technique toAnalysis theRhizosphere Specific Microbial Community of Seven Hybrid Rice Cultivars[J]. Journal of Integrative Agriculture, 2012, 12(11): 1817-1827.

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UsingPhospholipid FattyAcid Technique toAnalysis theRhizosphere Specific Microbial Community of Seven Hybrid Rice Cultivars

UsingPhospholipid FattyAcid Technique toAnalysis theRhizosphere Specific Microbial Community of Seven Hybrid Rice Cultivars

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