Optimization and Characterization of Nicosulfuron-Degrading Enzyme from Bacillus subtilis Strain YB1

doi:10.1016/S1671-2927(00)8680

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (9): 1485-1492.DOI: 10.1016/S1671-2927(00)8680

Optimization and Characterization of Nicosulfuron-Degrading Enzyme from Bacillus subtilis Strain YB1

KANG Zhan-hai, Dong Jin-gao, ZHANG Jin-lin

1.College of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China
2.College of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.China

收稿日期:2011-11-28 出版日期:2012-09-01 发布日期:2012-09-09
通讯作者: Correspondence ZHANG Jin-lin, Tel: +86-312-7528575, E-mail: zhangjinlin@hebau.edu.cn
作者简介:KANG Zhan-hai, E-mail: kangzhanhai@hebau.edu.cn;
基金资助:
This research was supported by the National Natural Science Foundation of China (31171877) and the Youth Foundation Program for Science and Technology of Hebei Province, China (2010141).

Optimization and Characterization of Nicosulfuron-Degrading Enzyme from Bacillus subtilis Strain YB1

KANG Zhan-hai, Dong Jin-gao, ZHANG Jin-lin

1.College of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China
2.College of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.China

Received:2011-11-28 Online:2012-09-01 Published:2012-09-09
Contact: Correspondence ZHANG Jin-lin, Tel: +86-312-7528575, E-mail: zhangjinlin@hebau.edu.cn
About author:KANG Zhan-hai, E-mail: kangzhanhai@hebau.edu.cn;
Supported by:
This research was supported by the National Natural Science Foundation of China (31171877) and the Youth Foundation Program for Science and Technology of Hebei Province, China (2010141).

摘要/Abstract

摘要： A strain of Bacillus subtilis strain YB1, isolated and preserved in our lab., showed a high nicosulfuron-degrading activity. Optimization of culture conditions on production of nicosulfuron-degrading enzyme from Bacillus subtilis strain YB1 was carried out through mono-factor experiments. The characterization of degrading enzyme(s) was studied in this paper. The results showed that B. subtilis YB1 can use nicosulfuron as sole carbon source under aerobic condition. The key enzyme(s) involved in the initial biodegradation of nicosulfuron was localized to extracellular proteins and showed to be induced expressed. Enzyme-specific activity was up to 89.34 U mg-1 at pH 8.0 and 30°C, incubation for 96 h, inoculum 4.5×108 CFU mL-1 in Luria-Bertani liquid medium with nicosulfuron of 40 mg L-1. The maximum degradation rate of extracellular crude enzymes on nicosulfuron was 66% at pH 9.0, 35°C in the enzymatic reaction system with nicosulfuron of 5 mg L-1. This degrading enzyme(s) was sensitive to high temperature, but kept high activity under alkaline conditions.

关键词: Bacillus subtilis, nicosulfuron, microbial degradation, enzyme

Abstract: A strain of Bacillus subtilis strain YB1, isolated and preserved in our lab., showed a high nicosulfuron-degrading activity. Optimization of culture conditions on production of nicosulfuron-degrading enzyme from Bacillus subtilis strain YB1 was carried out through mono-factor experiments. The characterization of degrading enzyme(s) was studied in this paper. The results showed that B. subtilis YB1 can use nicosulfuron as sole carbon source under aerobic condition. The key enzyme(s) involved in the initial biodegradation of nicosulfuron was localized to extracellular proteins and showed to be induced expressed. Enzyme-specific activity was up to 89.34 U mg-1 at pH 8.0 and 30°C, incubation for 96 h, inoculum 4.5×108 CFU mL-1 in Luria-Bertani liquid medium with nicosulfuron of 40 mg L-1. The maximum degradation rate of extracellular crude enzymes on nicosulfuron was 66% at pH 9.0, 35°C in the enzymatic reaction system with nicosulfuron of 5 mg L-1. This degrading enzyme(s) was sensitive to high temperature, but kept high activity under alkaline conditions.

Key words: Bacillus subtilis, nicosulfuron, microbial degradation, enzyme

KANG Zhan-hai, Dong Jin-gao, ZHANG Jin-lin. Optimization and Characterization of Nicosulfuron-Degrading Enzyme from Bacillus subtilis Strain YB1[J]. Journal of Integrative Agriculture, 2012, 12(9): 1485-1492.

参考文献

[1]Arias-Estévez M, López-Periagoa E, Martínez-Carballob E, Simal-Gándarab J, Mejutoc J C, García-Ríod L. 2008. The mobility and degradation of pesticides in soils and the pollution of groundwater resources. Agriculture, Ecosystems and Environment, 123, 247-260.

[2]Basaran P, Ozcan M. 2008. Characterization of β-xylosidase enzyme from a Pichia stipitis mutant. Bioresour Technology, 99, 38-43.

[3]Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.

[4]Cai T, Qian L H, Cai S, Chen L W. 2011. Biodegradation of benazolin-ethyl by strain Methyloversatilis sp. cd-1 isolated from activated sludge. Current Microbiology, 62, 570-577.

[5]Gaultier J, Farenhorst A, Cathcart J, Goddard T. 2008. Degradation of [carboxyl-14C] 2,4-D and [ring-U-14C ] 2, 4-D in 114 agricultural soil as affected by soil organic carbon content. Soil Biology and Biochemistry, 40, 217-227.

[6]Gu J G, Fan Y Z, Gu J D. 2003. Biodegradability of atrazine, cyanazine and dicamba under methanogenic condition in three soils of China. Chemosphere, 52, 1515-1521.

[7]Gu L F, Jiang J D, Li X H, Ali S W, Li S P. 2007. Biodegradation of ethametsulfuron-methyl by Pseudomonas sp. SW4 isolated from contaminated soil. Current Microbiology, 55, 420-426.

[8]Gupta V, Gaind S, Verma P K, Sood N, Srivastava A K. 2010. Purification and characterization of intracellular nitrilases from Rhodococcus sp. potential role of periplasmic nitrilase. African Journal of Micrbiology Research, 4, 1148-1153.

[9]Huang X, He J, Sun J Q, Pan J J, Sun X F, Li S P. 2007. Isolation and characterization of a metsulfuron-methyl degrading bacterium Methylopila sp. S113. International Biodeterioration and Biodegradation, 60, 152-158.

[10]Koskinen W, Regitano J. 2008. Characterization of nicosulfuron availability in aged soils. Journal of Agricultural and Food Chemistry, 56, 5801-5805.

[11]Liu Y H, Chung Y C, Xiong Y. 2001. Purification and characterization of a dimethoate-degrading enzyme of Aspergillus niger ZHY256, isolated from sewage. Applied and Environmental Microbiology, 67, 3764-3749.

[12]Luo W, Zhao Y H, Ding H T, Lin X Y, Zheng H B. 2008. Cometabolic degradation of bensulfuron-methyl in laboratory conditions. Journal of Hazardous Materials, 158, 208-214.

[13]Ma J P, Wang Z, Lu P, Wang H, Ali S, Li S, Huang X. 2009. Biodegradation of the sulfonylurea herbicide chlorimuron-ethyl by the strain Pseudomonas sp. LW3. FEMS Microbiology Letters, 296, 203-209.

[14]Nélieu S, Perreau F, Guichon R, Seguin F, Bry C, Einhorn J. 2005. Monitoring and effects of nicosulfuron in aquatic mesocosms: development of a simple analytical procedure and evidence for low toxicity to phytoplankton communities. Analytical and Bioanalytical Chemistry, 382, 108-114.

[15]Pérez-Pascual D, Gómez E, Álvarez B, Méndez J, Reimundo P, Navais R, Duchaud E, Guijarro J. 2011. Comparative analysis and mutation effects of fpp2-fpp1 tandem genes encoding proteolytic extracellular enzymes of Flavobacterium psychrophilum. Microbiology, 157, 1196-1204.

[16]Phillips T M, Seech A G, Lee H, Trevors J T. 2005. Biodegradation of hexachlorocyclohexane (HCH) by microorganisms. Biodegradation, 16, 363-392.

[17]Pizzul L, Castillo M, Stenstr J. 2009. Degradation of glyphosate and other pesticides by ligninolytic enzymes. Biodegradation, 20, 751-759.

[18]Sun W L, Chen Y P, Liu L X, Tang J, Chen J, Liu P. 2010. Conidia immobilization of T-DNA inserted Trichoderma atroviride mutant AMT-28 with dichlorvos degradation ability and exploration of biodegradation mechanism. Bioresource Technology, 101, 9197-9203.

[19]Yu Y L, Wang X, Luo M Y, Yang J F, Yu J Q, Fan D F. 2005. Fungal degradation of metsulfuron-methyl in pure cultures and soil. Chemosphere, 60, 460-466.

[20]Wackett L. 2009. Questioning our perceptions about evolution of biodegradative enzymes. Current Opinion in Microbiology, 12, 244-251.

[21]Xu J, Li X S, Xu Y J, Qiu L H, Pan C P. 2009. Biodegradation of pyrazosulfuron-ethyl by three strains of bacteria isolated from contaminated soils. Chemosphere, 74, 682-687.

[22]Zablotowicz R M, Weave M A, Locke M A. 2006. Microbial adaptation for accelerated atrazine mineralization/ degradation in Mississippi Delta soils. Weed Science, 54, 538-547.

[23]Zhang H, Zhang X, Mu W, Wang J, Pan H, Li Y. 2010. Biodegradation of chlorimuron-ethyl by the bacterium Klebsiella jilinsis 2N3. Journal of Environmental Science and Health (Part B-Pesticides Food Contamin), 45, 501-507.

Optimization and Characterization of Nicosulfuron-Degrading Enzyme from Bacillus subtilis Strain YB1

Optimization and Characterization of Nicosulfuron-Degrading Enzyme from Bacillus subtilis Strain YB1

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	WU Xin, LI Ze-yang, JIA An-feng, SU Hong-guang, HU Chun-hong, ZHANG Min-hong, FENG Jing-hai. Effects of high ambient temperature on lipid metabolism in finishing pigs[J]. Journal of Integrative Agriculture, 2016, 15(2): 391-396.
[2]	LI Qi-song, WU Lin-kun, CHEN Jun, Muhammad A Khan, LUO Xiao-mian, LIN Wen-xiong. Biochemical and microbial properties of rhizospheres under maize/ peanut intercropping[J]. Journal of Integrative Agriculture, 2016, 15(1): 101-110.
[3]	SONG Bing-yan, SHI Jin-xiao, SONG Song-quan. Dormancy release and germination of Echinochloa crus-galli grains in relation to galactomannan-hydrolysing enzyme activity[J]. Journal of Integrative Agriculture, 2015, 14(8): 1627-1636.
[4]	ZHANG Fei-fei, GAO Si, ZHAO Yuan-yuan, ZHAO Xiao-lei, LIU Xiao-man, XIAO Kai. Growth traits and nitrogen assimilation-associated physiological parameters of wheat (Triticum aestivum L.) under low and high N conditions[J]. Journal of Integrative Agriculture, 2015, 14(7): 1295-1308.
[5]	Nahla A Abdel-Aziz, Mounir El-Adawy, Maria A Mariezcurrena-Berasain, Abdelfattah Z M Salem, Jaime Olivares-Pérez, Ahmed E Kholif, Borhami E Borhami. Effects of exogenous enzymes, Lactobacillus acidophilus or their combination on feed performance response and carcass characteristics of rabbits fed sugarcane bagasse[J]. Journal of Integrative Agriculture, 2015, 14(3): 544-549.
[6]	Anita Bhatnagar, Ritu Lamba. Antimicrobial ability and growth promoting effects of feed supplemented with probiotic bacterium isolated from gut microflora of Cirrhinus mrigala[J]. Journal of Integrative Agriculture, 2015, 14(3): 583-592.
[7]	XU Hai-cheng, CAI Tie, WANG Zhen-lin, HE Ming-rong. Physiological basis for the differences of productive capacity among tillers in winter wheat[J]. Journal of Integrative Agriculture, 2015, 14(10): 1958-1970.
[8]	LIU Huan, SONG Xi-jiao, NI Yue-qun, LU Li-na, ZHOU Xue-ping , WU Jian-xiang. Highly Sensitive and Specific Monoclonal Antibody-Based Serological Methods for Rice Ragged Stunt Virus Detection in Rice Plants and Rice Brown Planthopper Vectors[J]. Journal of Integrative Agriculture, 2014, 13(9): 1943-1951.
[9]	SHAO Xing-hua , ZHENG Jian-wei. Soil Organic Carbon, Black Carbon, and Enzyme Activity Under Long- Term Fertilization[J]. Journal of Integrative Agriculture, 2014, 13(3): 517-524.
[10]	YI Bing, ZHOU Yu-fei, GAO Ming-yue, ZHANG Zhuang, HAN Yi, YANG Guang-dong, XU Wenjuan, HUANG Rui-dong. Effect of Drought Stress During Flowering Stage on Starch Accumulation and Starch Synthesis Enzymes in Sorghum Grains[J]. Journal of Integrative Agriculture, 2014, 13(11): 2399-2406.
[11]	HE Yong, YANG Jing, ZHU Biao , ZHU Zhu-jun. Low Root Zone Temperature Exacerbates the Ion Imbalance and Photosynthesis Inhibition and Induces Antioxidant Responses in Tomato Plants Under Salinity[J]. Journal of Integrative Agriculture, 2014, 13(1): 89-99.
[12]	QIN Hua, ZHOU Shuang , ZHANG Yi-zheng. Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato[J]. Journal of Integrative Agriculture, 2013, 12(9): 1530-1539.
[13]	FANG Fang, ZHANG Zhao-qi, ZHANG Xue-lian, WU Zhen-xian, YIN Hui-fang , PANG Xuequn. Reduction in Activity/Gene Expression of Anthocyanin Degradation Enzymes in Lychee Pericarp is Responsible for the Color Protection of the Fruit by Heat and Acid Treatment[J]. Journal of Integrative Agriculture, 2013, 12(9): 1694-1702.
[14]	BAI Jian-hui, LIU Jing-hui, ZHANG Na, YANG Jun-heng, SA Ru-la , WU Lan. Effect of Alkali Stress on Soluble Sugar, Antioxidant Enzymes and Yield of Oat[J]. Journal of Integrative Agriculture, 2013, 12(8): 1441-1449.
[15]	YAN Qiu-yan, DUAN Zeng-qiang, MAO Jing-dong, LI Xun , DONG Fei. Low Root Zone Temperature Limits Nutrient Effects on Cucumber Seedling Growth and Induces Adversity Physiological Response[J]. Journal of Integrative Agriculture, 2013, 12(8): 1450-1460.