Mapping of Quantitative Trait Loci for Adult Plant Resistance to Stripe Rust in German Wheat Cultivar Ibis

doi:10.1016/S1671-2927(00)8572

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (4): 528-536.DOI: 10.1016/S1671-2927(00)8572

Mapping of Quantitative Trait Loci for Adult Plant Resistance to Stripe Rust in German Wheat Cultivar Ibis

BAI Bin, REN Yan, XIA Xian-chun, DU Jiu-yuan, ZHOU Gang, WU Ling, ZHU Hua-zhong, HEZhong-hu, WANG Cheng-she

1.State Key Laboratory of Crop Stress Biology in Arid Areas/College of Agronomy, Northwest A & F University, Yangling 712100, P.R.China
2.Institute of Crop Sciences, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3.Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, P.R.China
4.Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R.China
5.CIMMYT China Office/Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

收稿日期:2011-11-24 出版日期:2012-04-01 发布日期:2012-04-11
通讯作者: Correspondence HE Zhong-hu, Tel: +86-10-82108547, E-mail: zhhecaas@163.com; WANG Cheng-she,Mobile: 13992861692, E-mail: wangcs2008@126.com
作者简介:BAI Bin, Tel: +86-10-82108564, E-mail: bbaigau@163.com
基金资助:
This study was supported by the National Natural Science Foundation of China (30821140351) and China Agriculture Research System (CARS-3-1-3).

Mapping of Quantitative Trait Loci for Adult Plant Resistance to Stripe Rust in German Wheat Cultivar Ibis

BAI Bin, REN Yan, XIA Xian-chun, DU Jiu-yuan, ZHOU Gang, WU Ling, ZHU Hua-zhong, HEZhong-hu, WANG Cheng-she

1.State Key Laboratory of Crop Stress Biology in Arid Areas/College of Agronomy, Northwest A & F University, Yangling 712100, P.R.China
2.Institute of Crop Sciences, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3.Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, P.R.China
4.Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R.China
5.CIMMYT China Office/Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2011-11-24 Online:2012-04-01 Published:2012-04-11
Contact: Correspondence HE Zhong-hu, Tel: +86-10-82108547, E-mail: zhhecaas@163.com; WANG Cheng-she,Mobile: 13992861692, E-mail: wangcs2008@126.com
About author:BAI Bin, Tel: +86-10-82108564, E-mail: bbaigau@163.com
Supported by:
This study was supported by the National Natural Science Foundation of China (30821140351) and China Agriculture Research System (CARS-3-1-3).

摘要/Abstract

摘要： The German wheat cultivar Ibis has excellent adult plant resistance (APR) to stripe rust in Gansu, a hotspot for stripe rust in China. To elucidate the genetic basis of APR to stripe rust in Ibis, 237 F3 lines derived from the cross Ibis/Huixianhong were evaluated at Tianshui, Gansu, in the 2008-2009 and 2009-2010 cropping seasons, and at Chengdu, Sichuan Province, China, in the 2009-2010 cropping season. Inoculations were conducted with a mixture of several prevalent Pst races in both locations. Maximum disease severity (MDS) data showed a continuous distribution of response, indicating quantitative nature of resistance to stripe rust in Ibis. The broad-sense heritability of MDS was 0.75 based on the mean values averaged across three environments. A total of 723 simple sequence repeat (SSR) markers were used to map the QTL for APR by inclusive composite interval mapping (ICIM). QTLs mapping to chromosomes 2BS and 6BS, designated as QYr.caas-2BS.1 and QYr.caas-6BS.1, respectively, explained 4.1-40.7% of the phenotypic variance in MDS across environments. The major effect QTL QYr.caas-2BS.1, flanked by Xgwm148 and Xwmc360, was consistently detected at all three sites as well as the averaged data over three environments, accounting for 40.7, 24.2, 5.2 and 29.9% of phenotypic variance, respectively. The molecular markers closely linked to this QTL have potential for use in marker-assisted selection and gene pyramiding to improve the durability of stripe rust resistance in wheat breeding.

关键词: Triticum aestivum, Puccinia striiformis, APR, simple sequence repeat

Abstract: The German wheat cultivar Ibis has excellent adult plant resistance (APR) to stripe rust in Gansu, a hotspot for stripe rust in China. To elucidate the genetic basis of APR to stripe rust in Ibis, 237 F3 lines derived from the cross Ibis/Huixianhong were evaluated at Tianshui, Gansu, in the 2008-2009 and 2009-2010 cropping seasons, and at Chengdu, Sichuan Province, China, in the 2009-2010 cropping season. Inoculations were conducted with a mixture of several prevalent Pst races in both locations. Maximum disease severity (MDS) data showed a continuous distribution of response, indicating quantitative nature of resistance to stripe rust in Ibis. The broad-sense heritability of MDS was 0.75 based on the mean values averaged across three environments. A total of 723 simple sequence repeat (SSR) markers were used to map the QTL for APR by inclusive composite interval mapping (ICIM). QTLs mapping to chromosomes 2BS and 6BS, designated as QYr.caas-2BS.1 and QYr.caas-6BS.1, respectively, explained 4.1-40.7% of the phenotypic variance in MDS across environments. The major effect QTL QYr.caas-2BS.1, flanked by Xgwm148 and Xwmc360, was consistently detected at all three sites as well as the averaged data over three environments, accounting for 40.7, 24.2, 5.2 and 29.9% of phenotypic variance, respectively. The molecular markers closely linked to this QTL have potential for use in marker-assisted selection and gene pyramiding to improve the durability of stripe rust resistance in wheat breeding.

Key words: Triticum aestivum, Puccinia striiformis, APR, simple sequence repeat

BAI Bin, REN Yan, XIA Xian-chun, DU Jiu-yuan, ZHOU Gang, WU Ling, ZHU Hua-zhong, HEZhong-hu, WANG Cheng-she. Mapping of Quantitative Trait Loci for Adult Plant Resistance to Stripe Rust in German Wheat Cultivar Ibis [J]. Journal of Integrative Agriculture, 2012, 12(4): 528-536.

BAI Bin, REN Yan, XIA Xian-chun, DU Jiu-yuan, ZHOU Gang, WU Ling, ZHU Hua-zhong, HEZhong-hu , WANG Cheng-she. Mapping of Quantitative Trait Loci for Adult Plant Resistance to Stripe Rust in German Wheat Cultivar Ibis [J]. Journal of Integrative Agriculture, 2012, 12(4): 528-536.

参考文献

[1]Bansal U K, Hayden M J, Keller B, Wellings C R, Park R F, Bariana H S. 2009. Relationship between wheat rust resistance genes Yr1 and Sr48 and a microsatellite marker. Plant Pathology, 58, 1039-1043.

[2]Börner A, Schumann E, Fürste A, Cöster H, Leithold B, Röder M S, Weber W E. 2002. Mapping of quantitative trait loci determining agronomic important characters in hexaploid wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 105, 921-936.

[3]Boukhatem N, Baret P V, Mingeot D, Jacquemin J M. 2002. Quantitative trait loci for resistance against yellow rust in two wheat-derived recombinant inbred line populations. Theoretical and Applied Genetics, 104, 111-118.

[4]Carter A H, Chen X M, Garland-Campbell K, Kidwell K K. 2009. Identifying QTL for high-temperature adult-plant resistance to stripe rust (Puccinia striiformis f. sp. tritici) in the spring wheat (Triticum aestivum L.) cultivar ‘Louise’. Theoretical and Applied Genetics, 119, 1119-1128.

[5]Chen W Q, Wu L R, Liu T G, Xu S C, Jin S L, Peng Y L, Wang B T. 2009. Race dynamics, diversity, and virulence evolution in Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Disease, 93, 1093-1101.

[6]Chen X M. 2005. Epidemiology and control of stripe rust (Puccinia striiformis f. sp. tritici) on wheat. Canadian Journal of Plant Pathology, 27, 314-337.

[7]Christiansen M J, Feenstra B, Skovgaard I M, Andersen S B. 2006. Genetic analysis of resistance to yellow rust in hexaploid wheat using a mixture model for multiple crosses. Theoretical and Applied Genetics, 112, 581-591.

[8]Fu D L, Uauy C, Distelfeld A, Blechl A, Epstein L, Chen X M, Sela H, Fahima T, Dubcovsky J. 2009. A kinase-START gene confers temperature-dependent resistance to wheat stripe rust. Science, 323, 1357-1360.

[9]Guo Q, Zhang Z J, Xu Y B, Li G H, Feng J, Zhou Y. 2008. Quantitative trait loci for high-temperature adult-plant and slow-rusting resistance to Puccinia striiformis f. sp. tritici in wheat cultivars. Phytopathology, 98, 803-809.

[10]Gupta P K, Balyan H S, Edwards K J, Isaac P, Korzun V, Röder M, Gautier M F, Joudrier P, Schlatter A R, Dubcovsky J, et al. 2002. Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theoretical and Applied Genetics, 105, 413-422.

[11]Guyomarc’h H, Sourdille P, Edwards K J, Bernard M. 2002. Studies of the transferability of microsatellites derived from Triticum tauschii to hexaploid wheat and to diploid related species using amplification, hybridization and sequence comparisons. Theoretical and Applied Genetics, 105, 736-744.

[12]He Z H, Lan C X, Chen X M, Zou Y C, Zhuang Q S, Xia X C. 2011. Progress and perspective in research of adultplant resistance to stripe rust and powdery mildew in wheat. Scientia Agricultura Sinica, 44, 2193-2215. (in Chinese)

[13]Hovmøller M S. 2007. Sources of seedling and adult plant resistance to Puccinia striiformis f. sp. tritici in European wheats. Plant Breeding, 126, 225-233.

[14]Jagger L J, Newell C, Berry S T, MacCormack R, Boyd L A. 2011. The genetic characterisation of stripe rust resistance in the German wheat cultivar Alcedo. Theoretical and Applied Genetics, 122, 723-733.

[15]Kang Z S, Zhao J, Han D J, Zhang H C, Wang X J, Wang C F, Han Q M, Guo J, Huang L L. 2011. Status of wheat rust research and control in China. BGRI 2010 Technical Workshop, St Petersburg, Russia. [2011-11-25]. http:// rustopedia.org/db/attachments/bgriiwc/24/2/07-kangca-A4-embargo.pdf

[16]Kosambi D D. 1944. The estimation of map distance from recombination values. Annals of Eugenics, 12, 172-175.

[17]Krattinger S G, Lagudah E S, Spielmeyer W, Singh R P, Huerta-Espino J, McFadden H, Bossolini E, Selter L L, Keller B. 2009. A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science, 323, 1360-1363.

[18]Lan C X, Liang S S, Zhou X C, Zhou G, Lu Q L, Xia X C, He Z H. 2010. Identification of genomic regions controlling adult-plant stripe rust resistance in Chinese landrace Pingyuan 50 through bulked segregant analysis. Phytopathology, 100, 313-318.

[19]Li H H, Ye G Y, Wang J K. 2007. A modified algorithm for the improvement of composite interval mapping. Genetics, 175, 361-374.

[20]Lillemo M, Asalf B, Singh R P, Huerta-Espino J, Chen X M, He Z H, Bjørnstad Å. 2008. The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theoretical and Applied Genetics, 116, 1155-1166.

[21]Lincoln S E, Daly M J, Lander E S. 1992. Constructing genetic maps with Mapmaker/EXP3.0. In: Whitehead Institute for Biomedical Research Technical Report, 3rd ed. Whitehead Institute, Cambridge. Lin F, Chen X M. 2009. Quantitative trait loci for non-racespecific, high-temperature adult-plant resistance to stripe rust in wheat cultivar Express. Theoretical and Applied Genetics, 118, 631-642.

[22]Lin F, Xu S C, Zhang L J, Miao Q, Zhai Q, Li N. 2005. SSR marker of wheat stripe rust resistance gene Yr2. Journal of Triticeae Crops, 25, 17-19.

[23]Li Z F, Xia X C, Zhou X C, Niu Y C, He Z H, Zhang Y, Li G Q, Wan A M, Wang D S, Chen X M, et al. 2006. Seedling and slow rusting resistance to stripe rust in Chinese common wheats. Plant Disease, 90, 1302-1312.

[24]Lu Y M, Lan C X, Liang S S, Zhou X C, Liu D, Zhou G, Lu Q L, Jing J X, Wang M N, Xia X C et al. 2009. QTL mapping for adult-plant resistance to stripe rust in Italian common wheat cultivars Libellula and Strampelli. Theoretical and Applied Genetics, 119, 1349-1359.

[25]Lowe I, Cantu D, Dubcovsky J. 2011. Durable resistance to the wheat rusts: integrating systems biology and traditional phenotype-based research methods to guide the deployment of resistance genes. Euphytica, 179, 69-79.

[26]Mallard S, Gaudet D, Aldeia A, Abelard C, Besnard A L, Sourdille P, Dedryver F. 2005. Genetic analysis of durable resistance to yellow rust in bread wheat. Theoretical and Applied Genetics, 110, 1401-1409.

[27]McIntosh R A, Dubcovsky J, Rogers W J, Morris C F, Appels R, Xia X C. 2010. Catalogue of gene symbols for wheat: 2010 supplement. [2011-10-20]. http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/ supplement2010.pdf

[28]McIntosh R A, Dubcovsky J, Rogers W J, Morris C F, Appels R, Xia X C. 2011. Catalogue of gene symbols for wheat: 2011 supplement. [2011-10-20]. http://www. shigen.nig.ac.jp/wheat/komugi/genes/macgene/ supplement2011.pdf

[29]McIntosh R A, Wellings C R, Park R F. 1995. Wheat Rusts: an Atlas of Resistance Genes. Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia. pp. 9-184.

[30]Park R F. 2008. Breeding cereals for rust resistance in Australia. Plant Pathology, 57, 591-602.

[31]Peterson R F, Campbell A B, Hannah A E. 1948. A diagrammatic scale for estimating rust intensity of leaves and stems of cereals. Canadian Journal of Research, 26, 496-500.

[32]Ramburan V P, Pretorius Z A, Louw J H, Boyd L A, Smith P H, Boshoff W H P, Prins R. 2004. A genetic analysis of adult plant resistance to stripe rust in the wheat cultivar Kariega. Theoretical and Applied Genetics, 108, 1426-1433.

[33]Richardson K L, Vales M I, Kling J G, Mundt C C, Hayes P M. 2006. Pyramiding and dissecting disease resistance QTL to barley stripe rust. Theoretical and Applied Genetics, 113, 485-495.

[34]Röder M S, Korzun V, Wendehake K, Plaschke J, Tixier M H, Leroy P, Ganal M W. 1998. A microsatellite map of wheat. Genetics, 149, 2007-2023.

[35]Rosewarne G M, Singh R P, Huerta-Espino J, William H M, Bouchet S, Cloutier S, McFadden H, Lagudah E S. 2006. Leaf tip necrosis, molecular markers and ?1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theoretical and Applied Genetics, 112, 500-508.

[36]Rosewarne G M, Singh R P, Huerta-Espino J, Rebetzke G J. 2008. Quantitative trait loci for slow-rusting resistance in wheat to leaf rust and stripe rust identified with multienvironment analysis. Theoretical and Applied Genetics, 116, 1027-1034.

[37]Santra D K, Chen X M, Santra M, Campbell K G, Kidwell K K. 2008. Identification and mapping QTL for hightemperature adult-plant resistance to stripe rust in winter wheat (Triticum aestivum L.) cultivar ‘Stephens’. Theoretical and Applied Genetics, 117, 793-802.

[38]Singh R P, Huerta-Espino J, Rajaram S. 2000. Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathologica et Entomologica Hungarica, 35, 133-139.

[39]Singh R P, Rajaram S. 1994. Genetics of adult plant resistance to stripe rust in ten spring bread wheats. Euphytica, 72, 1-7.

[40]Somers D J, Isaac P, Edwards K. 2004. A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 109, 1105-1114.

[41]Song Q J, Fickus E W, Cregan P B. 2002. Characterization of trinucleotide SSR motifs in wheat. Theoretical and Applied Genetics, 104, 286-293.

[42]Sourdille P, Singh S, Cadalen T, Brown-Guedira G L, Gay G, Qi L L, Gill B S, Dufour P, Murigneux A, Bernard M. 2004. Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Functional and Integrative Genomics, 4, 12-25.

[43]Stubbs R W. 1977. Observations on horizontal resistance to yellow rust (Puccinia striiformis f. sp. tritici). Cereal Rusts Bulletin, 5, 27-32.

[44]Suenaga K, Singh R P, Huerta-Espino J, William H M. 2003. Microsatellite markers for genes Lr34/Yr18 and other quantitative trait loci for leaf rust and stripe rust resistance in bread wheat. Phytopathology, 93, 881-890.

[45]Uauy C, Brevis J C, Chen X M, Khan I, Jackson L, Chicaiza O, Distelfeld A, Fahima T, Dubcovsky J. 2005. Hightemperature adult-plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1. Theoretical and Applied Genetics, 112, 97-105.

[46]Wan A M, Chen X M, He Z H. 2007. Wheat stripe rust in China. Australian Journal of Agricultural Research, 58, 605-619.

[47]Wan A M, Wu L R, Jin S L, Jia Q Z, Yao G, Yang J X, Wang B T , Li G B, Yuan Z Y. 2002. Occurrence of wheat stripe rust and monitoring of physiological races of Puccinia striiformis f. sp. tritici in China in 2000-2001. Plant Protection, 28, 5-9. (in Chinese)

[48]Wan A M, Zhao Z H, Chen X M, He Z H, Jin S L, Jia Q Z, Yao G, Yang J X, Wang B T, Li G B et al. 2004. Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Disease, 88, 896-904.

[49]William H M, Singh R P, Huerta-Espino J, Palacios G, Suenaga K. 2006. Characterization of genetic loci conferring adult plant resistance to leaf rust and stripe rust in spring wheat. Genome, 49, 977-990.

[50]Wellings C. Global status of stripe rust. 2011. BGRI 2010 Technical Workshop, St Petersburg, Russia. [2011-10-20]. http://www.globalrust.org/db/attachments/bgriiwc/ 24/3/05-WellingsA4-ca-embargo.pdf

[51]Xu X D, Feng J, Lin R M, Khalid H, Xu S C, Lin F. 2011. Postulation of stripe rust resistance genes in 44 Chinese wheat cultivars. International Journal of Agriculture and Biology, 13, 665-670.

[52]Young N D. 1996. QTL mapping and quantitative disease resistance in plants. Annual Review of Phytopathology, 34, 479-501.

[53]Zeng S M, Luo Y. 2006. Long-distance spread and interregional epidemics of wheat stripe rust in China. Plant Disease, 90, 980-988.

[54]Zeng S M, Luo Y. 2008. Systems analysis of wheat stripe rust epidemics in China. European Journal of Plant Pathology, 121, 425-438.

[55]Zhou X C, Du J Y, Lu Q L. 2005. Study on selection of resistance sources to stripe rust and gene bank setup for stripe-rust-resistant wheat breeding. Journal of Triticeae Crops, 25, 6-12.

Mapping of Quantitative Trait Loci for Adult Plant Resistance to Stripe Rust in German Wheat Cultivar Ibis

Mapping of Quantitative Trait Loci for Adult Plant Resistance to Stripe Rust in German Wheat Cultivar Ibis

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