RNA介导的DNA甲基化路径在植物抗病中的研究进展

doi:10.3864/j.issn.0578-1752.2015.S.002

摘要/Abstract

摘要： 在植物中，小分子RNA介导的DNA甲基化（RNA-directed DNA methylation，RdDM）是一个表观修饰过程，主要依赖两个核心蛋白：切割长双链RNA产生小分子干扰RNA（siRNA）的Dicer-Like 3（DCL3）蛋白和结合siRNA发挥功能的Argonaute 4（AGO4）蛋白。同时，RdDM的转录机制主要依赖两个植物特有的RNA聚合酶Pol IV和Pol V。近来，越来越多的研究表明RdDM路径参与植物防御反应。文章主要综述RdDM及其相关基因与植物抗病的最新研究进展，以期对作物抗病分子机制和育种提供有益的借鉴。

关键词: RdDM, siRNA, 表观修饰, 植物抗病

Abstract: RNA-directed DNA methylation (RdDM) is an epigenetic process in plants that relies on two core proteins: Dicer-Like 3 (DCL3), which processes long double-stranded RNAs (dsRNAs) into siRNAs, and Argonaute 4 (AGO4), which is involved in siRNA effector functions. RdDM also depends on a specialized transcriptional machinery that is centred around two plant-specific RNA polymerase II (Pol II)-related enzymes called Pol IV and Pol V. Recently, RdDM is found to be involved in plant defense. In this paper, we present an up-to-date overview on RdDM and its related genes during plant defense. We also provide opinions on future research directions for crop defense and breeding.

Key words: RdDM, siRNA, epigenetic modification, plant defense

耿帅锋，李爱丽，毛龙. RNA介导的DNA甲基化路径在植物抗病中的研究进展[J]. 中国农业科学, 2015, 48(S): 16-22.

GENG Shuai-feng, LI Ai-li, MAO Long. Research Progress on the Roles of RNA-Directed DNA Methylation Pathway in Plant Defense[J]. Scientia Agricultura Sinica, 2015, 48(S): 16-22.

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