关键词: 胚胎, 孤雌激活, 发育相关基因, 猪

Abstract:

【Objective】 This study was conducted to explore the molecular mechanism of early porcine development and find new candidate genes in the development of in vivo and parthenogenetically activated early porcine embryos.【Method】 The single preimplantation embryo differential display reverse transcription polymerase chain reaction (SPEDDRT-PCR) was used to identify differentially expressed genes in 2-cell, 4-cell, 8-16-cell in in vivo and parthenogenetically activated early porcine embryos. Differentially expressed genes were characterized by reverse Northern dot blot. 【Result】 A total of 11 ESTs (expressed sequences tags) were found using SPEDDRT-PCR and reverse Northern dot blot approach, three of which in 2-cell in vivo, five in 4-cell, one in 8-cell embryos, and the other two in 2-cell parthenogenetically activated embryos. All 11 ESTs were compared with nucleotide sequences deposited in the nr and dbEST database of Genbank using BLAST. DDD3 and DDD11 ESTs had no significant similarity with exisiting genes or ESTs, and were regarded as new ESTs. The two new ESTs were submitted to GenBank (accession numbers: EU597224, EU597228). The other ESTs had their highly similar nucleotide sequences with ESTs existing in nucleotide databases (but with unknown functions). 【Conclusion】 These results can provide information on gene expression patterns and lay a foundation for further study on the biological mechanisms controlling gene expression during preimplantation porcine embryo development.

Key words: embryos, parthenogenetic activation, development-related genes, pig