Ca2+和Ca2+-ATPase在小麦颖果筛分子分化中的动态变化

doi:10.3864/j.issn.0578-1752.2009.12.011

中国农业科学 ›› 2009, Vol. 42 ›› Issue (12): 4209-4217 .doi: 10.3864/j.issn.0578-1752.2009.12.011

• 耕作栽培·生理生化·农业信息技术 • 上一篇下一篇

Ca2+和Ca2+-ATPase在小麦颖果筛分子分化中的动态变化

李继伟,邓祥宜,周竹青,王利凯,阳超男,樊海燕

（华中农业大学生命科学技术学院）

收稿日期:2009-03-16 修回日期:1900-01-01 出版日期:2009-12-10 发布日期:2009-12-10
通讯作者: 周竹青

Dynamic Changes of Ca2+ and Ca2+-ATPase in Sieve Elements in the Developing Caryopsis of Triticum aestivum L.

LI Ji-wei, DENG Xiang-yi, ZHOU Zhu-qing, WANG Li-kai, YANG Chao-nan, FAN Hai-yan

（华中农业大学生命科学技术学院）

Received:2009-03-16 Revised:1900-01-01 Online:2009-12-10 Published:2009-12-10
Contact: ZHOU Zhu-qing

摘要/Abstract

摘要：

【目的】探讨Ca2+ 和Ca2+-ATPase在小麦颖果筛分子（sieve elements,SEs）分化过程中的动态变化及其在SEs的细胞程序性死亡（programmed cell death,PCD）中的作用。【方法】用透射电子显微术观察小麦颖果韧皮部分化过程中的超微结构变化;用Ca2+特异性荧光染色法和焦锑酸钾沉淀法,对小麦颖果韧皮部分化过程中的Ca2+进行组织和亚细胞水平的定位;同时用铅盐沉淀法对Ca2+-ATPase进行定位。【结果】超微结构观察发现,在SEs发育初期,细胞壁逐渐加厚,且内壁呈突起状,随着分化的进行,SEs细胞壁较以前明显变薄且平滑。Ca2+荧光试验表明,花后6～10 d,SEs细胞壁中有Ca2+的积累,其中花后9 d,SEs细胞壁Ca2+浓度最高;到花后14 d,细胞壁Ca2+浓度下降至对照水平。Ca2+亚细胞定位表明,在SEs中,花后1～2 d Ca2+主要分布在细胞膜上和细胞核中;花后4 d,SEs细胞质中Ca2+浓度增加,并且线粒体中也出现Ca2+颗粒;但到花后5～8 d,Ca2+主要分布在SEs细胞壁中,此时线粒体中未发现Ca2+颗粒;在花后10～18 d,Ca2+再次从细胞壁转移到胞内;花后20 d,SEs中Ca2+消失。在中间细胞（intermediary cells,ICs）中,花后1～18 d始终都有Ca2+颗粒,主要分布在细胞内壁上和液泡中。在SEs发育过程中,Ca2+-ATPase的活性发生显著变化。花后3 d时,SEs中的Ca2+-ATPase活性最弱;花后4～14 d SEs有较强的Ca2+-ATPase活性,且主要分布在SEs的细胞壁、细胞膜、胞间连丝等部位和线粒体、细胞核等细胞器上。【结论】Ca2+和Ca2+-ATPase在小麦颖果SEs的分化过程中呈动态变化,Ca2+可能参与介导了SEs的PCD过程。此外,Ca2+和Ca2+-ATPase可能对SEs细胞壁的加厚和SEs的功能实施有一定调控作用。

关键词: 小麦（Triticum aestivum L.）, 颖果, 筛分子, Ca2+, Ca2+-ATPase

Abstract:

【Objective】 Previous study revealed that sieve elements (SEs) in the developing caryopsis of Triticum aestivum L. underwent a unique type of programmed cell death (PCD) . In this paper, the dynamic changes and the roles of Ca2+ and Ca2+-ATPase in SEs during the PCD were studied. 【Method】 The ultrastructural aspects of phloem cells in wheat caryopsis were examined by transmission electron microscopy (TEM). Using specific fluorescence staining and potassium pyroantimonate precipitation method, Ca2+ was localized at histological and sub-cellular levels in SEs in the developing wheat caryopsis. TEM and lead nitrate were used to locate Ca2+-ATPase in SEs. 【Result】 TEM studies showed that the cell walls of SEs thickened at the beginning of differentiation, and then became thinner and smoother. Fluorescence staining showed that the fluorescence due to Ca2+ appeared in cell walls of SEs from 6 to 10 d after flowering. The fluorescence due to Ca2+ in cell walls of SEs was most notable on 9 d after flowering and disappeared on 14 d after flowering. Sub-celluar localization of Ca2+ showed that Ca2+ was localized on plasma membrane and in nuclei from 1 to 2 d after flowering. On 4 d after flowering, Ca2+ was localized in cytoplasm and mitochondria of SEs. From 5 to 8 d after flowering, Ca2+ was transported to the cell walls of SEs and no Ca2+ precipitates were observed in mitochondria. From 10 to 18 d after flowering, Ca2+ was transported into the cytoplasm again from cell walls and no Ca2+ precipitates were observed on 20 d after flowering. In intermediary cells (ICs), Ca2+ precipitates were observed from 1 to 18 d after flowering, and Ca2+ mainly distributed on intine and tonoplast. The activity of Ca2+-ATPase changed obviously during the SEs differentiation. There was lowest activity of Ca2+-ATPase on 3 d after flowering in SEs. High levels of Ca2+-ATPase activity were found from 4 to 14 d after flowering in SEs, and the enzyme was mainly localized in cell walls, cytoplasm, plasmodesmata, mitochondria and nuclei. 【Conclusion】 These results showed the dynamic changes of Ca2+ and Ca2+-ATPase in SEs differentiation. Ca2+ might play important roles in SEs during the PCD. In addition, Ca2+ and Ca2+-ATPase might be also related to cell wall thickening and functions of SEs.

Key words: wheat (Triticum aestivum L.), caryopsis, sieve elements, Ca2+, Ca2+-ATPase

李继伟,邓祥宜,周竹青,王利凯,阳超男,樊海燕 . Ca2+和Ca2+-ATPase在小麦颖果筛分子分化中的动态变化[J]. 中国农业科学, 2009, 42(12): 4209-4217 .

LI Ji-wei,DENG Xiang-yi,ZHOU Zhu-qing,WANG Li-kai,YANG Chao-nan,FAN Hai-yan
. Dynamic Changes of Ca2+ and Ca2+-ATPase in Sieve Elements in the Developing Caryopsis of Triticum aestivum L.
[J]. Scientia Agricultura Sinica, 2009, 42(12): 4209-4217 .

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2009.12.011

https://www.chinaagrisci.com/CN/Y2009/V42/I12/4209

[1]	郭丽1, 郭程瑾1, 路文静2, 李小娟2, 肖凯1. 磷转运蛋白基因TaPHT2;1在染色体上定位及对小麦磷素吸收和利用效率的影响[J]. 中国农业科学, 2014, 47(4): 613-621.
[2]	胡笛1, 2, 徐兆师2, 崔晓玉2, 陈明2, 李连城2, 马有志2, 张小红1. 小麦盐胁迫响应基因TaSRP的克隆及功能鉴定[J]. 中国农业科学, 2014, 47(12): 2292-2299.
[3]	梁亮, 张连蓬, 林卉, 李春梅, 杨敏华. 基于导数光谱的小麦冠层叶片含水量反演[J]. 中国农业科学, 2013, 46(1): 18-29.
[4]	钟俐, 李冠. 白粉病菌胁迫下甜瓜叶片中Ca2+的细胞化学定位及外源Ca2+对POD、CAT和SOD同功酶的影响[J]. 中国农业科学, 2012, 45(19): 4040-4049.
[5]	任永哲, 徐艳花, 贵祥卫, 王素平, 丁锦平, 张庆琛, 马原松, 裴冬丽. 盐胁迫下调控小麦苗期性状的QTL分析[J]. 中国农业科学, 2012, 45(14): 2793-2800.
[6]	杨凤军,李天来,臧忠婧,鲁少尉 . 外源钙施用时期对缓解盐胁迫番茄幼苗伤害的作用[J]. 中国农业科学, 2010, 43(6): 1181-1188 .
[7]	孙清鹏,于涌鲲,万善霞,赵福宽,郝玉兰 . 胞外及胞内Ca2+共同参与拟南芥中茉莉酸诱导的钙动员[J]. 中国农业科学, 2010, 43(5): 942-948 .
[8]	张振华,刘强,宋海星,荣湘民,Abdelbagi M.Ismail . K+,Ca2+和Mg2+对不同水稻(Oryza sativa L.)基因型苗期耐盐性的影响 [J]. 中国农业科学, 2010, 43(15): 3088-3097 .
[9]	李小娟,孙昭华,柯忻,路文静,郭程瑾,谷俊涛,肖凯 . 小麦锌指蛋白基因TaZAT6的克隆、特征分析及其在不同磷水平下的表达[J]. 中国农业科学, 2009, 42(9): 3339-3345 .
[10]	李罗江,茹振刚,高庆荣,姜辉,郭凤芝,吴世文,孙哲 . BNS小麦的雄性不育性及其温光特性[J]. 中国农业科学, 2009, 42(9): 3019-3027 .
[11]	王燕,吴振宇,杜艳丽,师光禄,陈梅,王海香,王有年 . 牵牛子种子提取物对朱砂叶螨触杀活性的测定[J]. 中国农业科学, 2009, 42(8): 2793-2800 .
[12]	路文静,李瑞娟,王效颖,李小娟,郭程瑾,谷俊涛,肖凯 . 响应低磷胁迫的小麦MDP激酶基因TaMPK1a-1的克隆和表达分析[J]. 中国农业科学, 2009, 42(7): 2601-2607 .
[13]	周竹青,李继伟,邓祥宜,王利凯,梅方竹,邹礼平 . 小麦颖果韧皮部细胞ATPase活性及其与籽粒光合同化物积累关系[J]. 中国农业科学, 2009, 42(7): 2314-2325 .
[14]	. 光敏核不育水稻花药中Ca2+分布及其与花粉育性的相关性研究[J]. 中国农业科学, 2009, 42(3): 809-815 .
[15]	. Ca2+信号参与铝诱导黑麦根系分泌有机酸的调控[J]. 中国农业科学, 2008, 41(8): 2279-2285 .

Ca2+和Ca2+-ATPase在小麦颖果筛分子分化中的动态变化

Dynamic Changes of Ca2+ and Ca2+-ATPase in Sieve Elements in the Developing Caryopsis of Triticum aestivum L.

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0