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1. Construction of a high-density adzuki bean genetic map and evaluation of its utility based on a QTL analysis of seed size
WANG Li-xia, WANG Jie, LUO Gao-ling, YUAN Xing-xing, GONG Dan, HU Liang-liang, WANG Su-hua, CHEN Hong-lin, CHEN Xin, CHENG Xu-zhen
Journal of Integrative Agriculture    2021, 20 (7): 1753-1761.   DOI: 10.1016/S2095-3119(20)63343-3
摘要163)      PDF    收藏

小豆(Vigna angularis (Willd.) Ohwi & Ohashi) 属于豇豆属亚洲豇豆亚属,是东亚各国传统种植作物。小豆营养丰富、医食两用,消费市场逐渐遍布全球。然而,小豆的遗传研究相对缓慢,导致育种技术落后、效率低下,难以满足生产和市场的需求。本研究基于高通量基因组测序技术构建了小豆SNP高密度遗传连锁图谱,该图谱共11条连锁群,含2904个标记,每条连锁群的标记数从208个(LG7)到397(LG1)个不等。图谱总长1365.0cM,标记间平均距离0.47cM,每条连锁群的长度从97.4cM(LG9)到155.6cM(LG1)不等。利用该图谱共发掘到两个与籽粒大小有关的主效QTL,分别位于LG2(22.1%) 和LG 9(18.8%)。此外,基于InDel侧翼序列,进一步开发了9718对引物,并随机选择200对进行PCR扩增检验,结果显示有75对在24份小豆种质中具有多态性。本研究中高密度图谱构建及籽粒大小的QTL 分析将进一步提升小豆重要性状基因的发掘等,而InDel标记的开发将有效促进小豆种质资源的遗传多样性分析、基因初步定位等研究。


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2. Advances in salinity tolerance of soybean: Genetic diversity, heredity, and gene identification contribute to improving salinity tolerance
CHEN Hua-tao, LIU Xiao-qing, ZHANG Hong-mei, YUAN Xing-xing, GU He-ping, CUI Xiao-yan, CHEN Xin
Journal of Integrative Agriculture    2018, 17 (10): 2215-2221.   DOI: 10.1016/S2095-3119(17)61864-1
摘要381)      PDF(pc) (725KB)(460)    收藏
Salt stress is one of the major abiotic stresses affecting soybean growth.  Genetic improvement for salt tolerance is an effective way to protect soybean yield under salt stress conditions.  Successful improvement of salt tolerance in soybean relies on identifying genetic variation that confers tolerance in soybean germplasm and subsequently incorporating these genetic resources into cultivars.  In this review, we summarize the progress in genetic diversity and genetics of salt tolerance in soybean, which includes identifying genetic diversity for salt tolerant germplasm; mapping QTLs conferring salt tolerance; map-based cloning; and conducting genome-wide association study (GWAS) analysis in soybean.  Future research avenues are also discussed, including high throughput phenotyping technology, the CRISPR/Cas9 Genome-Editing System, and genomic selection technology for molecular breeding of salt tolerance.
 
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3. Whole-genome identification and expression analysis of K+ efflux antiporter (KEA) and Na+/H+ antiporter (NHX) families under abiotic stress in soybean
CHEN Hua-tao, CHEN Xin, WU Bing-yue, YUAN Xing-xing, ZHANG Hong-mei, CUI Xiao-yan
Journal of Integrative Agriculture    2015, 14 (6): 1171-1183.   DOI: 10.1016/S2095-3119(14)60918-7
摘要2849)      PDF    收藏
Sodium toxicity and potassium insufficient are important factors affecting the growth and development of soybean in saline soil. As the capacity of plants to maintain a high cytosolic, K+/Na+ ratio is the key determinant of tolerance under salt stress. The aims of the present study were to identify and analyse expression patterns of the soybean K+ efflux antiporter (KEA) gene and Na+/H+ antiporter (NHX) gene family, and to explore their roles under abiotic stress. As a result, 12 soybean GmKEAs genes and 10 soybean GmNHXs genes were identified and analyzed from soybean genome. Interestingly, the novel soybean KEA gene Glyma16g32821 which encodes 11 transmembrane domains were extremely up-regulated and remained high level until 48 h in root after the excessive potassium treatment and lack of potassium treatment, respectively. The novel soybean NHX gene Glyma09g02130 which encodes 10 transmembrane domains were extremely up-regulated and remained high level until 48 h in root with NaCl stress. Imaging of subcellular locations of the two new Glyma16g32821-GFP and Glyma09g02130-GFP fusion proteins indicated all plasma membrane localizations of the two novel soybean genes. The 3D structures indicated that the two soybean novel proteins Glyma09g02130 (NHX) and Glyma16g32821 (KEA) all belong to the cation/hydrogen antiporter family.
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