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1. 干扰苯丙氨酸解氨酶基因pal1延迟糙皮侧耳发育的分子机制
Qi He, Yuqing Jiang, Chenyang Huang, Lijiao Zhang, Ludan Hou, Fangjie Yao, Mengran Zhao
Journal of Integrative Agriculture    2025, 24 (4): 1477-1488.   DOI: 10.1016/j.jia.2024.06.002
摘要52)      PDF    收藏

食用菌发育受阻会影响子实体的生产周期和产量。苯丙氨酸解氨酶(PALEC 4.3.1.5是一种催化苯丙氨酸脱氨生成反式肉桂酸的酶。前期研究结果发现,糙皮侧耳pal1基因的转录降低能够延缓子实体发育因此,我们以野生型(WT)RNA干扰(RNAi)菌株为材料,利用转录组测序和农杆菌介导的遗传转化方法,研究了pal1基因的分子调控机制。结果表明,干扰pal1基因导致PAL酶活性和总酚含量下降胞内H2O2含量增加。RNA-Seq数据表明,KEGG通路显著富集在过氧化物酶途径、MAPK信号途径-酵母和另外三条途径,编码过氧化氢酶的基因cat1参与了上述显著富集的多个通路。外源H2O2能够显著增强cat1基因的转录,提高CAT总酶活性。添加H2O2清除剂后,RNAi-pal1菌株的cat1基因转录水平和CAT酶活性显著高于野生型菌株,表明pal1通过影响胞内的H2O2含量来调节cat1的表达。过表达糙皮侧耳cat1基因导致了生长迟缓,尤其是在原基形成过程中。综上所述,本研究阐明了PAL1通过信号分子H2O2影响cat1基因的表达从而调控了糙皮侧耳的发育。研究结果深化了对食用菌分子发育机制的理解。

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2. 尤力克柠檬锌指蛋白ClDOF3.4与柑橘黄化脉明病毒外壳蛋白互作抑制病毒侵染
Ping Liao, Ting Zeng, Mengyang Huangfu, Cairong Zheng, Jiequn Ren, Changyong Zhou, Yan Zhou
Journal of Integrative Agriculture    2024, 23 (6): 1979-1993.   DOI: 10.1016/j.jia.2024.03.049
摘要81)      PDF    收藏
柑橘黄化脉明病毒(citrus yellow vein clearing virusCYVCV)是一种新型柑橘病毒,该病毒已对印度、伊朗、土耳其、中国、美国和韩国的柠檬、酸橙和部分宽皮等柑橘品种的种植生产造成了严重的损失。CYVCV外壳蛋白(CP)具有RNA沉默抑制子活性,与病毒致病性和症状密切相关。DOFDNA-binding with one finger)是高等植物特有的锌指蛋白,在植物生长发育和抗病原物侵染的过程中扮演着重要作用。然而,目前尚未开展尤力克柠檬锌指蛋白ClDOFCYVCV的互作研究。因此,本研究通过一系列体内和体外互作验证实验,以及基因瞬时表达、基因瞬时沉默和尤力克柠檬遗传转化等手段,验证了ClDOF3.4CYVCV CP之间的互作关系及其对尤力克柠檬响应CYVCV免疫应答的影响。试验结果表明,ClDOF3.4蛋白在体内和体外均与CP存在相互作用关系,且ClDOF3.4蛋白N端的104-280个氨基酸序列是与CP相互作用的关键区段。在尤力克柠檬叶片中瞬时表达ClDOF3.4可以诱导水杨酸通路相关基因和超敏反应标记基因的上调表达,造成接种部位活性氧爆发和离子渗透坏死、并引起H2O2和水杨酸积累;而ClDOF3.4基因瞬时沉默尤力克柠檬叶片中的水杨酸和超敏反应相关基因下调表达。此外,该研究进一步通过农杆菌接种分别在瞬时表达及沉默ClDOF3.4基因的尤力克柠檬叶片中瞬时表达CP,结果表明ClDOF3.4负向调控CP在尤力克柠檬中的表达和积累。接种CYVCV六个月后,ClDOF3.4转基因尤力克柠檬植株的CYVCV含量约为对照植株的69.4%,与对照植株相比,转基因植株感病后的症状明显减轻,仅出现轻度的叶片斑驳症状。上述研究表明,ClDOF3.4不仅与CYVCV-CP存在相互作用,还可以通过水杨酸信号通路诱导尤力克柠檬对CYVCV的免疫应答。这是首次关于锌指蛋白参与柑橘抗病毒病的报道,该研究结果为进一步研究柑橘抗CYVCV的分子机制奠定了基础。
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3. JIA-2021-0204 玉米-豆科间套作通过改变根系空间分布、豆科结瘤能力和土壤氮素有效性来促进氮素吸收
ZHENG Ben-chuan, ZHOU Ying, CHEN Ping, ZHANG Xiao-na, DU Qing, YANG Huan, WANG Xiao-chun, YANG Feng, XIAO Te, LI Long, YANG Wen-yu, YONG Tai-wen
Journal of Integrative Agriculture    2022, 21 (6): 1755-1771.   DOI: 10.1016/S2095-3119(21)63730-9
摘要188)      PDF    收藏

通过2年定位试验研究不同玉米-豆科间套作模式下(玉米-大豆带状套作、玉米-花生带状间作、玉米净作、大豆净作和花生净作)作物氮素吸收与根系分布、豆科结瘤和土壤氮素有效性之间的关系。结果表明:与净作相比,间套作显著降低了单季作物的单位面积吸氮量,但玉米-大豆套作和玉米-花生间作的系统总吸氮量分别增加31.7-45.4%和7.4-12.2%。间套作显著增加了玉米和大豆的单株氮素吸收量,与净作相比分别增加61.6%和31.8%,间作花生的单株吸氮量较净作降低46.6%。间套作系统中玉米和大豆的根系呈现不对称性分布,其根长密度和根表面密度显著高于相应单作。间作花生受竞争抑制,其根表面密度显著低于相应单作。与净作相比,套作大豆的根瘤数量和根瘤鲜重显著增加,间作花生根瘤数和根瘤鲜重则显著降低。间套作显著提高了玉米和大豆的土壤酶活性(蛋白酶、脲酶、硝酸还原酶)和土壤有效氮含量,但降低了花生的土壤酶和土壤有效氮含量。玉米-大豆带状套作系统比玉米-花生带状间作系统更有利于氮素吸收,玉米与豆科间套作可以促进玉米对氮素的吸收,从而降低氮肥用量,提高农业可持续性。


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4. Emergence of H5N1 highly pathogenic avian influenza in Democratic People’s Republic of Korea
LIU Li-ling, YANG Huan-liang, GUO Fu-sheng, WANG Xiu-rong, DENG Guo-hua, SHI Jian-zhong, TIAN Guo-bin, ZENG Xian-ying
Journal of Integrative Agriculture    2022, 21 (5): 1534-1538.   DOI: 10.1016/S2095-3119(21)63829-7
摘要154)      PDF    收藏
在过去的几十年,国内外研究人员对动物和人感染高致病性禽流感进行了全球范围的广泛监测,然而有关北朝鲜的禽流感流行病学研究数据却很少。在2013和2014年,在北朝鲜的家禽中多次暴发高致病性禽流感,我们分离到H5N1亚型高致病性禽流感病毒,进化分析显示分离到的2株病毒的HA基因高度同源,均属于2.3.2.1c分支,我们分析认为引起2014年鸡场禽流感暴发的病毒可能是由2013年Tudan鸭场病毒经迁徙野鸟引入。本实验的数据提供了禽流感病毒可以由野鸟-水禽-陆禽进行传播的直接证据。因此,我们必需加强水禽禽流感的监测和控制,这对预防和控制高致病性禽流感至关重要。


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5. 番茄GDSL酯酶/脂肪酶基因的全基因组鉴定和表达分析
SUN Yao-guang, HE Yu-qing, WANG He-xuan, JIANG Jing-bin, YANG Huan-huan, XU Xiang-yang
Journal of Integrative Agriculture    2022, 21 (2): 389-406.   DOI: 10.1016/S2095-3119(20)63461-X
摘要480)      PDF    收藏

GDSL酯酶/脂肪酶家族包含许多功能基因,它们在植物的生长发育、形态建成、种子油脂合成和防御反应中发挥重要的生物学功能。GDSL酯酶/脂肪酶基因可响应生物和非生物胁迫。虽然GDSL酯酶/脂肪酶家族基因在其他植物中已被鉴定和研究,但它们在番茄中的分类和功能尚不清楚。本研究首次在番茄中鉴定了80GDSL酯酶/脂肪酶家族基因成员,命名为SlGELP1-80我们对这些基因在染色体上的位置进行了定位,并对它们的理化性质、基因结构、系统发育关系、共线关系和顺式作用元件进行了分析。SlGELP基因在番茄中的时空表达特征具有多样性。此外,结合RNA-seq分析表明,番茄接种Stemphylium lycopersici前后SlGELP基因的表达模式不同。用qRT-PCR方法验证番茄接种S. lycopersici病原菌以及分别喷施SAJA处理后5显著差异SlGELP基因的表达。本研究首次利用生物信息学方法鉴定和分析了番茄GDSL酯酶/脂肪酶家族基因,为提高植物抗病性研究提供了新的思路。

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6. Effects of post-silking water deficit on the leaf photosynthesis and senescence of waxy maize
YE Yu-xiu, WEN Zhang-rong, YANG Huan, LU Wei-ping, LU Da-lei
Journal of Integrative Agriculture    2020, 19 (9): 2216-2228.   DOI: 10.1016/S2095-3119(20)63158-6
摘要152)      PDF    收藏
Waxy maize is widely cultivated under rainfed conditions and frequently suffers water shortage during the late growth stage.  In this study, a pot trial was conducted to examine the effects of post-silking drought on leaf photosynthesis and senescence and its influence on grain yield.  Two waxy maize hybrids, Suyunuo 5 (SYN5) and Yunuo 7 (YN7), were grown under the control and drought (soil moisture content was 70–80% and 50–60%, respectively) conditions after silking in 2016 and 2017.  The decrease in yield was 11.1 and 15.4% for YN7 and SYN5, respectively, owing to the decreased grain weight and number.  Post-silking dry matter accumulation was reduced by 27.2% in YN7 and 26.3% in SYN5.  The contribution rate of pre-silking photoassimilates transferred to grain yield was increased by 15.6% in YN7 and 10.2% in SYN5, respectively.  Post-silking drought increased the malondialdehyde content, but decreased the contents of water, soluble protein, chlorophyll, and carotenoid in the leaves.  The weakened activities of enzymes involved in photosynthesis (ribulose-1,5-bisphosphate carboxylase and phosphoenolpyruvate carboxylase) and antioxidant system (catalase, superoxide dismutase and peroxidase) reduced the photosynthetic rate (Pn) and accelerated leaf senescence.  The correlation results indicated that reduced Pn and catalase activity and increased malondialdehyde content under drought conditions induced the decrease of post-silking photoassimilates deposition, ultimately resulted in the grain yield loss.
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7. Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato
WANG Zi-yu, bAO Yu-fang, PEI Tong, WU Tai-ru, DU Xu, HE Meng-xi, WANG Yue, LIU Qi-feng, YANG Huan-huan, JIANG Jing-bin, ZHANG He, LI Jing-fu, ZHAO Ting-ting, XU Xiang-yang
Journal of Integrative Agriculture    2020, 19 (11): 2699-2708.   DOI: 10.1016/S2095-3119(20)63350-0
摘要128)      PDF    收藏
BRI1-EMS-SUPPRESSOR 1 (BES1) transcription factor is closely associated with the brassinosteroid (BR) signaling pathway and plays an important role in plant growth and development.  SLB3 is a member of BES1 transcription factor family and its expression was previously shown to increase significantly in tomato seedlings under drought stress.  In the present study,we used virus-induced gene silencing (VIGS) technology to downregulate SLB3 expression to reveal the function of the SLB3 gene under drought stress further.  The downregulated expression of SLB3 weakened the drought tolerance of the plants appeared earlier wilting and higher accumulation of H2O2 and O2·, decreased superoxide dismutase (SOD) activity, and increased proline (PRO) and malondialdehyde (MDA) contents and peroxidase (POD) activity.  Quantitative real-time PCR (qRT-PCR) analysis of BR-related genes revealed that the expression of SlCPD, SlDWARF and BIN2-related genes was significantly upregulated in SLB3-silenced seedlings under drought stress, but that the expression of TCH4-related genes was downregulated.  These results showed that silencing the SLB3 gene reduced the drought resistance of tomato plants and had an impact on the BR signaling transduction which may be probably responsible for the variation in drought resistance of the tomato plants. 
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8. Weakened carbon and nitrogen metabolisms under post-silking heat stress reduce the yield and dry matter accumulation in waxy maize
YANG Huan, GU Xiao-tian, DING Meng-qiu, LU Wei-ping, LU Da-lei
Journal of Integrative Agriculture    2020, 19 (1): 78-88.   DOI: 10.1016/S2095-3119(19)62622-5
摘要187)      PDF    收藏
Post-silking high temperature is one of the abiotic factors that affects waxy maize (Zea mays L. sinensis Kulesh) growth in southern China.  We conducted a pot trial in 2016–2017 to study the effects of post-silking daytime heat stress (35°C) on the activities of enzymes involved in leaf carbon and nitrogen metabolisms and leaf reactive oxygen species (ROS) and water contents.  This study could improve our understanding on dry matter accumulation and translocation and grain yield production.  Results indicated that decreased grain number and weight under heat stress led to yield loss, which decreased by 20.8 and 20.0% in 2016 and 2017, respectively.  High temperature reduced post-silking dry matter accumulation (16.1 and 29.5% in 2016 and 2017, respectively) and promoted translocation of pre-silking photoassimilates stored in vegetative organs, especially in leaf.  The lower leaf water content and chlorophyll SPAD value, and higher ROS (H2O2 and O2-·) content under heat stress conditions indicated accelerated senescent rate.  The weak activities of phosphoenolpyruvate carboxylase (PEPCase), Ribulose-1,5-bisphosphate carboxylase (RuBPCase), nitrate reductase (NR), and glutamine synthase (GS) indicated that leaf carbon and nitrogen metabolisms were suppressed when the plants suffered from a high temperature during grain filling.  Correlation analysis results indicated that the reduced grain yield was mainly caused by the decreased leaf water content, weakened NR activity, and increased H2O2 content.  The increased accumulation of grain weight and post-silking dry matter and the reduced translocation amount in leaf was mainly due to the increased chlorophyll SPAD value and NR activity.  Reduced PEPCase and RuBPCase activities did not affect dry matter accumulation and translocation and grain yield.  In conclusion, post-silking heat stress down-regulated the leaf NR and GS activities, increased the leaf water loss rate, increased ROS generation, and induced pre-silking carbohydrate translocation.  However, it reduced the post-silking direct photoassimilate deposition, ultimately, leading to grain yield loss.
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9. Downregulation of SL-ZH13 transcription factor gene expression decreases drought tolerance of tomato
ZHAO Ting-ting, WANG Zi-yu, BAO Yu-fang, ZHANG Xiao-chun, YANG Huan-huan, ZHANG Dong-ye, JIANG Jing-bin, ZHANG He, LI Jing-fu, CHEN Qing-shan, XU Xiang-yang
Journal of Integrative Agriculture    2019, 18 (7): 1579-1586.   DOI: 10.1016/S2095-3119(19)62621-3
摘要293)      PDF    收藏
Zinc finger-homeodomain proteins (ZF-HDs) are transcription factors that regulate plant growth, development, and abiotic stress tolerance.  The SL-ZH13 gene was found to be significantly upregulated under drought stress treatment in tomato (Solanum lycopersicum) leaves in our previous study.  In this study, to further understand the role that the SL-ZH13 gene plays in the response of tomato plants to drought stress, the virus-induced gene silencing (VIGS) method was applied to downregulate SL-ZH13 expression in tomato plants, and these plants were treated with drought stress to analyze the changes in drought tolerance.  The SL-ZH13 silencing efficiency was confirmed by quantitative real-time PCR (qRT-PCR) analysis.  In SL-ZH13-silenced plants, the stems wilted faster, leaf shrinkage was more severe than in control plants under the same drought stress treatment conditions, anyd the mean stem bending angle of SL-ZH13-silenced plants was smaller than that of control plants.  Physiological analyses showed that the activity of superoxide dismutase (SOD) and peroxidase (POD) and the content of proline (Pro) in SL-ZH13-silenced plants were lower than those in control plants after 1.5 and 3 h of drought stress treatment.  The malondialdehyde (MDA) content in SL-ZH13-silenced plants was higher than that in control plants after 1.5 and 3 h of drought stress treatment, and H2O2 and O2-· accumulated much more in the leaves of SL-ZH13-silenced plants than in the leaves of control plants.  These results suggested that silencing the SL-ZH13 gene affected the response of tomato plants to drought stress and decreased the drought tolerance of tomato plants. 
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10. Effects of high temperature during grain filling on physicochemical properties of waxy maize starch
LU Da-lei, YANG Huan, SHEN Xin, LU Wei-ping
Journal of Integrative Agriculture    2016, 15 (2): 309-316.   DOI: 10.1016/S2095-3119(15)61095-4
摘要1838)      PDF    收藏
Understanding the waxy maize starch physicochemical properties response to heat stress during grain filling could improve starch quality. The effects of heat stress during early (1–15 days after pollination, DAP) and late (16–30 DAP) grain filling stages on the starch physicochemical properties of four waxy maize varieties were evaluated. Crystallinity only increased in Suyunuo 5 after exposure to high temperature at late grain filling stage. The effects of heat stress on digestibility and swelling power were dependent on varieties and stages. Generally, swelling power was increased by heat stress at early grain development stage and digestibility was increased by high temperature at late grain filling stage, respectively. The results of correlation analysis indicated the starch with large granule size could swell well and easy digest. Peak, trough, final, and breakdown viscosities in response to heat stress were dependent on stages and varieties. In general, peak, trough and final viscosities were decreased and increased by heat stress at early grain formation and late grain filling stages, respectively; whereas the breakdown and setback viscosities were similar among the three treatments. Heat stress increased the gelatinization temperatures and retrogradation percentage. Gelatinization range decreased under heat stress at 1–15 DAP but remained constant under heat stress at 16–30 DAP in all varieties. The starch exposed to high temperature at 16–30 DAP presented higher digestibility and peak viscosity and lower retrogradation percentage than those at 1–15 DAP. Therefore, heat stress at early grain formation stage severely affects the physicochemical properties of starch.
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