Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato

doi:10.1016/S2095-3119(20)63350-0

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (11): 2699-2708.DOI: 10.1016/S2095-3119(20)63350-0

所属专题：园艺-分子生物合辑Horticulture — Genetics · Breeding

收稿日期:2020-02-03 出版日期:2020-11-01 发布日期:2020-10-15

Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato

WANG Zi-yu¹, BAO Yu-fang¹, PEI Tong¹, WU Tai-ru¹, DU Xu¹, HE Meng-xi¹, WANG Yue¹, LIU Qi-feng¹, YANG Huan-huan¹, JIANG Jing-bin¹, ZHANG He¹, LI Jing-fu¹, ZHAO Ting-ting¹, XU Xiang-yang^{1, 2}

¹ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, P.R.China
² Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, P.R.China

Received:2020-02-03 Online:2020-11-01 Published:2020-10-15
Contact: Correspondence ZHAO Ting-ting, Tel/Fax: +86-451-55190300, E-mail: ttzhao2016@163.com; XU Xiang-yang, E-mail: xxy709@126.com
About author:WANG Zi-yu, E-mail: 1533485512@qq.com;
Supported by:
This research was supported by the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province, China (UNPYSCT-2018169), the China Postdoctoral Science Foundation Grant (2018 M630333), the National Key R&D Program of China (2017YFD0101900), and the earmarked fund for China Agriculture Research System (CARS-23-A-16).

摘要/Abstract

Abstract:

BRI1-EMS-SUPPRESSOR 1 (BES1) transcription factor is closely associated with the brassinosteroid (BR) signaling pathway and plays an important role in plant growth and development. SLB3 is a member of BES1 transcription factor family and its expression was previously shown to increase significantly in tomato seedlings under drought stress. In the present study,we used virus-induced gene silencing (VIGS) technology to downregulate SLB3 expression to reveal the function of the SLB3 gene under drought stress further. The downregulated expression of SLB3 weakened the drought tolerance of the plants appeared earlier wilting and higher accumulation of H₂O₂ and O₂^–·, decreased superoxide dismutase (SOD) activity, and increased proline (PRO) and malondialdehyde (MDA) contents and peroxidase (POD) activity. Quantitative real-time PCR (qRT-PCR) analysis of BR-related genes revealed that the expression of SlCPD, SlDWARF and BIN2-related genes was significantly upregulated in SLB3-silenced seedlings under drought stress, but that the expression of TCH4-related genes was downregulated. These results showed that silencing the SLB3 gene reduced the drought resistance of tomato plants and had an impact on the BR signaling transduction which may be probably responsible for the variation in drought resistance of the tomato plants.

Key words: SLB3 , BES1 transcription factor , VIGS , drought , gene expression , BR signaling pathway

. [J]. Journal of Integrative Agriculture, 2020, 19(11): 2699-2708.

WANG Zi-yu, bAO Yu-fang, PEI Tong, WU Tai-ru, DU Xu, HE Meng-xi, WANG Yue, LIU Qi-feng, YANG Huan-huan, JIANG Jing-bin, ZHANG He, LI Jing-fu, ZHAO Ting-ting, XU Xiang-yang. Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato[J]. Journal of Integrative Agriculture, 2020, 19(11): 2699-2708.

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Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato

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