深翻秸秆还田在我国东北地区已得到了广泛的应用，可以显著增加土壤有机碳储量，改善底层土壤养分循环功能。土壤微生物普遍被认为是这一过程的关键，但其在深层土壤改良中的作用仍研究有限。本研究于2018年开始，以东北地区棕壤为研究对象，以常规耕作（CT，翻耕深度15 cm）为对照，分析了秸秆浅混还田（SCT，翻耕深度15 cm）、深翻处理（IT，翻耕深度35 cm）和深翻秸秆还田（SIT，翻耕深度35 cm）对土壤微生物群落网络和多养分循环功能的影响。结果表明，深翻秸秆还田改善了土壤多养分循环指数，提高了表层和底层土壤有机碳、全氮、有效氮、有效磷和有效钾等养分含量。与传统耕作和秸秆浅混还田相比，深翻秸秆还田通过减少微生物网络平均连通度和节点数，增加平均路径长度和模块化程度，创造了一个结构相对松散但具有高集中度集群的网络结构。同时随机森林分析发现，平均路径长度和聚类系数是影响土壤多养分循环功能的主要因素。综上，深翻秸秆还田将是改善棕壤养分循环和微生物群落结构的一个有效措施，以上结果也为该地区关于微生物驱动秸秆分解策略提供了重要信息。

It is generally known that the culture for mycoplasma is time-consuming and a variety of nutrients are needed in the culture medium.  This brings a lot of difficulties to mycoplasma research and application, including Mycoplasma mycoides subsp. capri (Mmc).  Furthermore, little research on the characteristics of Mmc metabolism has been reported.  In this study, Mmc PG3 strain cultures were investigated for dynamic gene expression.  Culture samples were harvested during logarithmic phase (PG3-1), stationary phase (PG3-2), decline phase (PG3-3) and late decline phase (PG3-4).  Twelve RNA samples (three replicates for each of the four growth stages considered) from these cultures were collected and sequenced.  Paired comparison between consecutive growth phases in the four growth stages showed 45 significant differentially expressed genes (P<0.01) were linked to PG3 metabolism.  The enzymes these genes coded were mainly involved in ATP synthase, pyrimidine metabolism, nicotinate and nicotinamide metabolism, arginine and proline metabolism.  Among these, cytidylate kinase, fructose 1,6-bisphosphate aldolases Class II, nicotinate-nucleotide adenylyltransferase and dihydrolipoamide dehydrogenase play a key role in Mmc metabolism.  These results provide a baseline to build our understanding of the metabolic pathway of Mmc.  

Mycoplasma ovipneumoniae, a kind of mycoplasma bacteria, commonly infects the respiratory tract causing respiratory disease in sheep and goats worldwide. Here, the complete genome sequence of M. ovipneumoniae strain NM2010 isolated from a sheep in China was reported for the first time.