Understanding the metabolism of Mycoplasma mycoides subsp. capri in vitro by a transcriptomic analysis

doi:10.1016/S2095-3119(17)61747-7

Journal of Integrative Agriculture ›› 2018, Vol. 17 ›› Issue (2): 428-435.DOI: 10.1016/S2095-3119(17)61747-7

收稿日期:2017-07-04 出版日期:2018-02-20 发布日期:2018-02-01

Understanding the metabolism of Mycoplasma mycoides subsp. capri in vitro by a transcriptomic analysis

WANG Xiao-hui^1*, WANG Yan-fang^{1, 2*}, HUANG Hai-bi¹, BAI Fan¹, SHI Xiao-na¹, MA Chang-jiao¹, GAO Yuan¹, ZHANG Jian-hua¹, ZHANG Wen-guang³, HAO Yong-qing¹

¹ Key Laboratory of Microbiology & Immunology, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, P.R.China
² Foundation Medicine College, Baotou Medical School, Baotou 014040, P.R.China
³ Key Laboratory of Animal Genetics and Genomics, Inner Mongolia Agricultural University, Hohhot 010018, P.R.China

Received:2017-07-04 Online:2018-02-20 Published:2018-02-01
Contact: Correspondence HAO Yong-qing, E-mail: haoyq1960@163.com; ZHANG Wen-guang, E-mail: atcgnmbi@aliyun.com
About author:WANG Xiao-hui, Mobile: +86-15034782080, E-mail: wangxiaohui_hao@126.com; WANG Yan-fang, Mobile: +86-15849279411, E-mail: wyf-nmgnd@163.com; * These authors contributed equally to this study.
Supported by:
This work was supported by the Science and Technology Project of Inner Mongolia, China (201502070).

摘要/Abstract

Abstract: It is generally known that the culture for mycoplasma is time-consuming and a variety of nutrients are needed in the culture medium. This brings a lot of difficulties to mycoplasma research and application, including Mycoplasma mycoides subsp. capri (Mmc). Furthermore, little research on the characteristics of Mmc metabolism has been reported. In this study, Mmc PG3 strain cultures were investigated for dynamic gene expression. Culture samples were harvested during logarithmic phase (PG3-1), stationary phase (PG3-2), decline phase (PG3-3) and late decline phase (PG3-4). Twelve RNA samples (three replicates for each of the four growth stages considered) from these cultures were collected and sequenced. Paired comparison between consecutive growth phases in the four growth stages showed 45 significant differentially expressed genes (P<0.01) were linked to PG3 metabolism. The enzymes these genes coded were mainly involved in ATP synthase, pyrimidine metabolism, nicotinate and nicotinamide metabolism, arginine and proline metabolism. Among these, cytidylate kinase, fructose 1,6-bisphosphate aldolases Class II, nicotinate-nucleotide adenylyltransferase and dihydrolipoamide dehydrogenase play a key role in Mmc metabolism. These results provide a baseline to build our understanding of the metabolic pathway of Mmc.

Key words: metabolism , Mycoplasma mycoides subsp. capri , transcriptomic

. [J]. Journal of Integrative Agriculture, 2018, 17(2): 428-435.

WANG Xiao-hui, WANG Yan-fang, HUANG Hai-bi, BAI Fan, SHI Xiao-na, MA Chang-jiao, GAO Yuan, ZHANG Jian-hua, ZHANG Wen-guang, HAO Yong-qing . Understanding the metabolism of Mycoplasma mycoides subsp. capri in vitro by a transcriptomic analysis[J]. Journal of Integrative Agriculture, 2018, 17(2): 428-435.

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Understanding the metabolism of Mycoplasma mycoides subsp. capri in vitro by a transcriptomic analysis

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