本研究在花椒中鉴定了270个MYB基因，并将其分为四个亚家族。 R2R3-MYB (ZbMYB) 包含251个基因，根据系统发育结果和序列相似性分为33个亚科。这些亚科中有24个同时包含花椒MYB和拟南芥AtMYB，9个仅包含花椒或拟南芥。具有相似功能的ZbMYB聚集在同一亚科中，表明功能保守。亚细胞定位分析预测大多数ZbMYB基因存在于细胞核中。转座复制在花椒MYB基因家族的扩展中起主要作用。通过系统发育分析和转录组分析，发现28个ZbMYB基因可能调控花椒中黄酮类化合物的生物合成，这些基因呈现出独特的时空表达模式。在花椒果实不同发育阶段，通过qRT-PCR分析得出EVM0042160和EVM0033809基因的表达模式与花椒中的黄酮类和花青素物质含量曲线非常相似。进一步相关性分析表明，不同发育阶段花椒果实的黄酮类物质含量与28个ZbMYB基因的转录本丰度具有不同程度的相关性。这些结果表明ZbMYB基因可能参与了花椒中黄酮类物质代谢途径。对为花椒中MYB基因家族全面系统分析以及进一步研究MYB转录因子的功能奠定了坚实的基础。

As the most important organ in plant photosynthesis, the leaf plays an important role in plant growth and development. Leaf senescence is associated with fundamental changes in the proteome. To research the molecular mechanisms of leaf senescence, protein expression in senescing maize ear leaves grown under field conditions was analyzed using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS). A total of 60 senescence-associated proteins were identified. The identified proteins are involved in many biological processes, especially energy, metabolism and protein synthesis. Several of the identified proteins have not been previously reported as senescence-associated, including glycine-rich RNA-binding protein.