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1. JIA-2022-0517 低温贮藏和留树保鲜期间血橙多酚含量、组成以及抗氧化活性的变化
ZHAO Ji-chun, AO Miao, HE Xiao-qin, LI Wei-zhou, DENG Li-li, ZENG Kai-fang, MING Jian
Journal of Integrative Agriculture    2022, 21 (12): 3669-3683.   DOI: 10.1016/j.jia.2022.09.011
摘要225)      PDF    收藏

柑橘类水果富含酚类化合物,对人体具有多种健康益处。然而,很少有研究关注这类水果在采后贮藏过程中酚类化合物的变化。本研究对低温贮藏和留树保鲜12周的Tarocco血橙的酚类含量、成分和抗氧化活性进行了动态监测,并通过评估苯丙烷途径相关酶活性和基因表达探讨了血橙酚类化合物变化机制。结果表明,黄烷酮是血橙中主要酚类化合物,两种贮藏方式主要通过促进酚酸的积累从而提高总酚含量,并在第12周达到最大值。留树保鲜血橙的酚类含量和抗氧化性要高于低温贮藏血橙。此外,苯丙烷途径相关酶活性和基因表达结果显示,贮藏期间血橙中酚类物质的积累与苯丙烷途径的激活具有高度相关性。以上结果表明,从酚类化合物的角度来看,留树保鲜储存是延长血橙供应期的一种潜在有效方式。

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2. Polypropylene crisper and 1-MCP delay the softening, lignification and transcription levels of related enzyme genes of golden needle mushrooms (Flammulina velutipes)
WANG Wen-jun, LI Yao, LI Fu-hua, ZENG Kai-fang, MING Jian
Journal of Integrative Agriculture    2022, 21 (1): 249-260.   DOI: 10.1016/S2095-3119(21)63764-4
摘要143)      PDF    收藏

采后新鲜金针菇孢含水量高,质地脆嫩,仍具有较高的生理活性和呼吸作用,从而极易发生衰老和品质劣化。本研究使用1-甲基环丙烯(1-MCP)和聚丙烯(PP)保鲜盒处理金针菇,分析了金针菇在贮藏过程中木质化和软化的变化情况。主要研究结果如下:PP保鲜盒包装能有效地延长金针菇的贮藏时间;1-MCP处理、PP保鲜盒包装及两种处理组合均能显著抑制木质素的积累,并降低纤维素、果胶含量,对木质化及软化相关酶(苯丙氨酸解氨酶(PAL)、肉桂醇脱氢酶(CAD)、纤维素酶(Cx)、果胶甲基酯酶(PME)和聚半乳糖醛酸(PG)活性有一定的抑制作用。其中,PP保鲜盒包装的抑制效果高于1-MCP处理,且两种处理组合效果最好。透射电镜(TEM)和扫描电镜(SEM)结果表明,PP保鲜盒包装和1-MCP处理相结合能有效地保持金针菇细胞结构的完整性和稳定性,防止质膜分离及细胞膜破裂。转录水平分析结果表明,PP保鲜盒包装和1-MCP处理相结合,能明显影响金针菇木质化和软化相关酶基因的表达。综上,1-MCPPP保鲜盒包装可以有效的延缓金针菇在贮藏过程中的木质化和软化现象,延长贮藏期。

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3. Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK
LI Fu-hua, ZHENG Shao-jie, ZHAO Ji-chun, LIAO Xia, WU Su-rui, MING Jian
Journal of Integrative Agriculture    2020, 19 (11): 2829-2838.   DOI: 10.1016/S2095-3119(20)63322-6
摘要126)      PDF    收藏
Morchella angusticeps Peck, one of the most popular edible mushrooms, has attracted great attention due to its delicious taste and healthy properties.  However, both its biological effects and the possible mechanism of action have not yet been known.  We investigated the anti-proliferative activity of the phenolic extract derived from Morchella angusticeps Peck (MPE) against HepG2 human hepatocellular carcinoma cells.  Results showed that MPE at non-cytotoxicity doses significantly inhibited the proliferation of HepG2 cells in a dose-dependent manner with inhibitory rates ranging from 18 to 90% (P<0.01).  The possible mechanism might be that MPE induced apoptosis through initiating the mitochondrial death pathway by regulating Bax, Bcl-2 and cleaved caspase-3.  On the other hand, MPE might trigger cell cycle arrest at G0/G1/S phases by managing p21, Cyclin D1, cyclin-dependent kinases-4 (CDK4) and proliferating cell nuclear antigen (PCNA).  Additionally, MPE downregulated TRAF-2 and p-p53, while upregulated p-ASK1 and p-p38.  Therefore, it could be inferred that MPE might induce the anti-proliferative function to HepG2 cells through the p38/MAPK signal transduction pathway.
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4. Comparison of phenolic profiles and antioxidant activities in skins and pulps of eleven grape cultivars (Vitis vinifera L.)
LI Fu-xiang, LI Fu-hua, YANG Ya-xuan, YIN Ran, MING Jian
Journal of Integrative Agriculture    2019, 18 (5): 1148-1158.   DOI: 10.1016/S2095-3119(18)62138-0
摘要230)      PDF    收藏
Eleven grape cultivars were analysed to explore the variety differences of fresh grape phenolic profiles.  The results showed that free phenolics were predominant in grape skins and pulps, and showed the higher antioxidant activities than bound.  In 11 cultivars, Muscat Kyoho extracts had the highest total phenolic content in skins (10.525 mg GAE g–1 FW) and pulps (1.134 mg GAE g–1 FW), and exhibited the highest DPPH radical scavening capacity (EC50=11.7 µg mL–1) and oxygen radical absorbance capacity (ORAC) value (190.57 µmol TE g–1 FW) of free phenolic in skin.  In addition, the most abundant phenolics in grape skins were found to be flavonoids such as kaempferol in Kyoho skin (541.2 µg g–1 FW), rutin, catechin and epicatechin in Muscat Kyoho skin (262.3, 86.3 and 70.0 µg g–1 FW, respectively).  Furthermore, the principal component analysis showed a strong difference of phenolic profiles with the cultivars, existing forms and distributions.  Pearson correlation coefficient analysis showed a significant linear correlation between total phenolic content and antioxidant activity (P<0.05).  Therefore, both skins and pulps were rich sources of bioactive phenolic compounds, and Muscat Kyoho was the ideal source among all samples.
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5. Mathematical Modeling and Effect of Various Hot-Air Drying on Mushroom (Lentinus edodes)
GUO Xiao-hui, XIA Chun-yan, TAN Yu-rong, CHEN Long , MING Jian
Journal of Integrative Agriculture    2014, 13 (1): 207-216.   DOI: 10.1016/S2095-3119(13)60265-8
摘要1793)      PDF    收藏
An experimental study was performed to determine the characteristics and drying process of mushroom (Lentinus edodes) by 6 different hot-air drying methods namely isothermal drying, uniform raise drying, non-uniform raise drying, uniform intermittent drying, non-uniform intermittent drying and combined drying. The chemical composition (dry matter, ash, crude protein, crude fat, total sugars, dietary fiber, and energy), color parameters (L, a*, b*, c*, and h0) and rehydration capacities were determined. Among all the experiments, non-uniform intermittent drying reached a better comprehensive results due to the higher chemical composition, better color quality associated with high bright (26.381±5.842), high color tone (73.670±2.975), low chroma (13.349±3.456) as well as the highest rehydration (453.76% weigh of dried body). Nine kinds of classical mathematical model were used to obtained moisture data and the Midili-kucuk model can be described by the drying process with the coefficient (R2 ranged from 0.99790 to 0.99967), chi-square (χ2 ranged from 0.00003 to 0.00019) and root mean square error (RMSE ranged from 0.000486 to 0.0012367).
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6. Phenolic Profiles and Antioxidant Activity of Buckwheat (Fagopyrum esculentum Möench and Fagopyrum tartaricum L. Gaerth) Hulls, Brans and Flours
LI Fu-hua, YUAN Ya, YANG Xiao-lan, TAO Shu-ying , MING Jian
Journal of Integrative Agriculture    2013, 12 (9): 1684-1693.   DOI: 10.1016/S2095-3119(13)60371-82012-10-19
摘要1700)      PDF    收藏
The extracts from hulls, brans and flours of Fagopyrum esculentum Möench (FEM, three varieties) and Fagopyrum tartaricum L. Gaerth (FTG, seven varieties) were screened for free and bound phenolic content or total phenolic content (TPC), as well as 1,1 diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity and reducing power. Free phenolics were predominant in buckwheat hulls, brans and flours. FEM hulls extract exhibited the highest reducing power and DPPH free radical scavenging activity with the average EC50 84.54 μg mL-1 and IC50 11.54 μg mL-1 respectively, FTG brans extract had the highest average TPC (24.87 mg GAE g-1 DW), and FEM flours extract showed the lowest TPC, reducing power and radical scavenging activity. Furthermore, the correlations among TPC, DPPH free radical scavenging activity and reducing power of all the samples were investigated. The rank correlation coefficient (rs) between reducing power and DPPH free radical scavenging activity of buckwheat hulls, between TPC and DPPH free radical scavenging activity of buckwheat flours were 0.76 and 0.79, respectively (P<0.05). However, there is no significant correlation between the remaining indexes of hulls and flours, as well as the ten buckwheat brans. This result indicated that some non-phenolic compounds also contributed to the total antioxidant activity in hulls, brans and flours of buckwheats. This study demonstrated that buckwheat hulls and brans, rather than flours, are good source of antioxidants.
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7. β-羟丁酸(BHB)通过ACSL4诱导奶牛乳腺上皮细胞脂肪酸含量增加与脂滴积累
Ming Li, Jingjing Wang, Jianan Wen, Juan J. Loor, Qianming Jiang, Jingyi Wang, Huijing Zhang, Yue Yang, Wei Yang, Bingbing Zhang, Chuang Xu
Journal of Integrative Agriculture    DOI: 10.1016/j.jia.2024.12.004
录用日期: 2024-12-06