Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK

doi:10.1016/S2095-3119(20)63322-6

Journal of Integrative Agriculture ›› 2020, Vol. 19 ›› Issue (11): 2829-2838.DOI: 10.1016/S2095-3119(20)63322-6

所属专题：食品科学合辑Food Science

收稿日期:2020-01-19 出版日期:2020-11-01 发布日期:2020-10-15

Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK

LI Fu-hua^{1, 2*}, ZHENG Shao-jie^1*, ZHAO Ji-chun^{1, 2}, LIAO Xia¹, WU Su-rui³, MING Jian^{1, 2}

¹ College of Food Science, Southwest University, Chongqing 400715, P.R.China
² Research Center of Food Storage & Logistics, Southwest University, Chongqing 400715, P.R.China
³ Kunming Edible Fungi Institute, All China Federation of Supply and Marketing Cooperatives, Kunming 650223, P.R.China

Received:2020-01-19 Online:2020-11-01 Published:2020-10-15
Contact: Correspondence MING Jian, Tel/Fax: +86-23-68251947, E-mail: mingjian1972@163.com
About author:LI Fu-hua, E-mail: fuhualee92@163.com; ZHENG Shao-jie, E-mail: 1654633013@qq.com; * These authors contributed equally to this study.
Supported by:
This research was supported by the National Natural Science Foundation of China (31471576, 31271825) and the Fundamental Research Funds for the Central Universities, China (XDJK2018B030, SWU118012).

摘要/Abstract

Abstract:

Morchella angusticeps Peck, one of the most popular edible mushrooms, has attracted great attention due to its delicious taste and healthy properties. However, both its biological effects and the possible mechanism of action have not yet been known. We investigated the anti-proliferative activity of the phenolic extract derived from Morchella angusticeps Peck (MPE) against HepG2 human hepatocellular carcinoma cells. Results showed that MPE at non-cytotoxicity doses significantly inhibited the proliferation of HepG2 cells in a dose-dependent manner with inhibitory rates ranging from 18 to 90% (P<0.01). The possible mechanism might be that MPE induced apoptosis through initiating the mitochondrial death pathway by regulating Bax, Bcl-2 and cleaved caspase-3. On the other hand, MPE might trigger cell cycle arrest at G₀/G₁/S phases by managing p21, Cyclin D1, cyclin-dependent kinases-4 (CDK4) and proliferating cell nuclear antigen (PCNA). Additionally, MPE downregulated TRAF-2 and p-p53, while upregulated p-ASK1 and p-p38. Therefore, it could be inferred that MPE might induce the anti-proliferative function to HepG2 cells through the p38/MAPK signal transduction pathway.

Key words: Morchella angusticeps Peck , phenolic extract , cell cycle , apoptosis , anti-proliferation

. [J]. Journal of Integrative Agriculture, 2020, 19(11): 2829-2838.

LI Fu-hua, ZHENG Shao-jie, ZHAO Ji-chun, LIAO Xia, WU Su-rui, MING Jian. Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK[J]. Journal of Integrative Agriculture, 2020, 19(11): 2829-2838.

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Phenolic extract of Morchella angusticeps peck inhibited the proliferation of HepG2 cells in vitro by inducing the signal transduction pathway of p38/MAPK

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