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1. First record of the golden potato nematode Globodera rostochiensis in Yunnan and Sichuan provinces of China
JIANG Ru, PENG Huan, LI Yun-qing, LIU Hui, ZHAO Shou-qi, LONG Hai-bo, HU Xian-qi, GE Jian-jun, LI Xing-yue, LIU Miao-yan, SHAO Bao-lin, PENG De-liang
Journal of Integrative Agriculture    2022, 21 (3): 898-899.   DOI: 10.1016/S2095-3119(21)63845-5
摘要461)      PDF    收藏

马铃薯孢囊线虫Globodera rostochiensis是国际公认的重要检疫性有害线虫,严重危害马铃薯。2018-2020年,在全国农业技术推广服务中心组织的全国马铃薯检疫性线虫调查中,从云南省昭通市鲁甸县和四川省越西县和昭觉县马铃薯根系发现金色孢囊线虫,经形态学观察鉴定、分子生物学rDNA-ITS及28S的D2-D3区域特征分析比对及种特异性引物ITS5和PITSr3检测确定为马铃薯金线虫Globodera rostochiensis(Wollenweber)Skarbilovich, 1959. 在隔离温室内采用盆栽人工接种的致病性研究结果表明,该三个金线虫种群都能侵染马铃薯(品种青薯9号)并繁殖,接种12周后,马铃薯上形成成熟雌虫,完成生活史。这是马铃薯金线虫在我国云南和四川省首次记录。


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2. Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene (Ha-acp1) from Heterodera avenae
LIU Yan-ke, HUANG Wen-kun, LONG Hai-bo, PENG Huan, HE Wen-ting , PENG De-liang
Journal of Integrative Agriculture    2014, 13 (6): 1303-1310.   DOI: 10.1016/S2095-3119(13)60536-5
摘要1699)      PDF    收藏
For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene (Ha-acp1) expressed in the subventral glands of the cereal cyst nematode (Heterodera avenae) was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated specifically in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.
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