Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (6): 1303-1310.DOI: 10.1016/S2095-3119(13)60536-5

所属专题: 线虫合辑Nematology

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Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene (Ha-acp1) from Heterodera avenae

 LIU Yan-ke, HUANG Wen-kun, LONG Hai-bo, PENG Huan, HE Wen-ting , PENG De-liang   

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
  • 收稿日期:2012-04-23 出版日期:2014-06-01 发布日期:2014-06-06
  • 通讯作者: PENG De-liang, E-mail: dlpeng@ippcaas.cn
  • 作者简介:LIU Yan-ke, E-mail: Shmilyake@126.com
  • 基金资助:

    the fund of the National Basic Research Program of China (2013CB127502), the Special Fund for Agro-Scientific Research in the Public Interest, China (200903040) and the National Natural Science Foundation of China (31201493).

Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene (Ha-acp1) from Heterodera avenae

 LIU Yan-ke, HUANG Wen-kun, LONG Hai-bo, PENG Huan, HE Wen-ting , PENG De-liang   

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
  • Received:2012-04-23 Online:2014-06-01 Published:2014-06-06
  • Contact: PENG De-liang, E-mail: dlpeng@ippcaas.cn
  • About author:LIU Yan-ke, E-mail: Shmilyake@126.com
  • Supported by:

    the fund of the National Basic Research Program of China (2013CB127502), the Special Fund for Agro-Scientific Research in the Public Interest, China (200903040) and the National Natural Science Foundation of China (31201493).

摘要: For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene (Ha-acp1) expressed in the subventral glands of the cereal cyst nematode (Heterodera avenae) was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated specifically in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.

关键词: Heterodera avenae , acid phosphatase , RNA interference

Abstract: For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene (Ha-acp1) expressed in the subventral glands of the cereal cyst nematode (Heterodera avenae) was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated specifically in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.

Key words: Heterodera avenae , acid phosphatase , RNA interference