蚕豆（Vicia faba L.）基因组较大（约13 Gb），尚无参考基因组，与其他豆类相比，蚕豆遗传研究落后很多。鉴于此，本研究选择三个蚕豆纯系（云豆8137、H0003712和H000572）作为亲本，构建了两个F₂群体，其中群体1（云豆8137×H0003712）包含167个单株，群体2（H000572×云豆8137）包含204个单株；利用Targeted next-generation sequencing（TNGS）基因分型平台，对两个F₂群体进行基因分型，构建了两个高密度的蚕豆SNP遗传连锁图谱。基于群体1构建的图谱包含5103个SNP标记，长度1333.31 cM，平均标记密度0.26 cM；基于群体2构建的图谱包含1904个SNP标记，覆盖长度1610.61 cM。利用上述两个遗传连锁图谱及两个F₂群体，挖掘了与蚕豆花、荚、株型和籽粒相关的14个农艺性状的98个QTLs。此外，本研究对上述两个遗传连锁图谱进行整合，构建了一张包含6895个SNP标记，覆盖长度3324.48 cM的整合图谱。本研究不仅为蚕豆相关基因的图位克隆奠定了基础，还有助于推动蚕豆分子标记辅助育种的发展。

分根区交替滴灌施肥(ADF)是将交替灌溉与滴灌施肥相结合的灌溉施肥技术，具有节水和提高氮肥利用率的潜力。通过研究ADF下不同施肥频率对土壤水分养分分布及番茄产量的影响，以期为设施番茄的生产提高理论依据。试验于2019-2020年5-10月进行，试验设置了3个ADF灌溉施肥频率F3（3d）、F6（6d）、F12（12d），并设常规滴灌施肥处理作为对照（CK，施肥频率为6天）；所有处理的总施氮量均为180 kg ha^-1。对于ADF处理，在植株两侧10 cm处分别放置2根滴灌带，通过滴灌带上配置的手动阀门实现交替滴灌施肥；对于CK处理，在番茄根部附近放置1根滴灌带。CK处理两年的总灌水量分别为450.6和446.1 mm；而ADF处理的灌水量为CK处理的60%。结果表明：随着滴灌施肥频率的增加，高频率的F3处理引起水分和无机N主要分布在0–40 cm土层，而40–60 cm土层较少。与CK处理相比，F6处理在0–20和20–40 cm土层无机N两年的平均含量分别增加21.0%和29.0%，而在40–60 cm土层降低23.0 %。F3、F6、F12和CK处理2年的平均番茄产量分别为107.5、102.6、87.2和98.7 t ha^-1。F3处理的番茄产量较F12处理显著高23.3 %，而F3和F6处理之间无显著差异。F6处理的番茄产量与CK处理无显著性差异，说明ADF在减少40%灌溉水的前提下能够保持番茄产量。综合考虑土壤水分养分分布以及番茄的产量，ADF条件下6天的施肥频率可以作为温室番茄生产中一种水肥调控管理策略。

Seven inorganic amendment materials were added into arsenic (As) contaminated soil at a rate of 0.5% (w/w); the materials used were sepiolite, red mud, iron grit, phosphogypsum, ferrihydrite, iron phosphate, and layered double oxides (LDO). Plant growth trials using rape (edible rape, Brassia campestris L.) as a bio-indicator are commonly used to assess As bioavailability in soils. In this study, B. campestris was grown in a contaminated soil for 50 days. All of the inorganic amendments significantly inhibited the uptake of As by B. campestris. Following soil treatment with the seven aforementioned inorganic ammendments, the As concentrations in the edible parts of B. campestris were reduced by 28.6, 10.5, 8.7, 31.0, 47.4, 25.3, and 28.8%, respectively, as compared with the plants grown in control soil. The most effective amendment was ferrihydrite, which reduced As concentration in B. campestris from 1.84 to 0.97 mg kg–1, compared to control. Furthermore, ferrihydrite-treated soils had a remarkable decrease in both non-specifically sorbed As and available-As by 67 and 20%, respectively, comparing to control. Phosphogypsum was the most cost-effective amendment and it showed excellent performance in reducing the water soluble As in soils by 31% and inhibiting As uptake in B. campestris by 21% comparing to control. Additionally, obvious differences in As transfer rates were observed in the various amendments. The seven amendment materials used in this study all showed potential reduction of As bioavailability and influence on plant growth and other biological processes still need to be further explored in the long term.

The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and b-catenin on the proliferation role and secretion function of bovine mammary epithelial cells cultured in vitro. Firstly, the epithelial character of bovine mammary epithelial cells was authenticated using immunofluorescence, then the cell grow curve was observed and investigated after S. vaccariae and T. mogono treatment. On the effect of S. vaccariae and T. mogono, cell adhesion molecules E-cadherin, b-catenin and CycinD1 mRNA and protein were detected by qRT-PCR and Western blotting, respectively. The results showed that the cellular keratin 18 expressed positively and proliferated vigorously after S. vaccariae and T. mogono treament. The mRNA and protein levels of E-cadherin and CycinD1 were remarkably higher (P<0.05) in 36 h after S. vaccariae and T. mogono treatment. The cell proliferation at 36 h was increased significantly (P<0.05). In conclusion, S. vaccariae and T. mogono have a positive impact on the cell proliferation and an effect on the adhesion molecules E-cadherin, b-catenin and CycinD1 in the Wnt signaling pathway.