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1. 细胞自噬相关基因BdATG8参与调控苹果轮纹病菌的发育和致病过程
LIU Na, LIAN Sen, ZHOU Shan-yue, WANG Cai-xia, REN Wei-chao, LI Bao-hua
Journal of Integrative Agriculture    2022, 21 (8): 2319-2328.   DOI: 10.1016/S2095-3119(21)63863-7
摘要222)      PDF    收藏

本研究鉴定并研究了苹果轮纹病菌的细胞自噬关键基因BdATG8。研究结果发现,BdATG8能够恢复酿酒酵母ATG8缺失突变体对氮源饥饿的耐受性缺陷。我们建立了检测轮纹病菌细胞自噬过程发生的有效标记(GFP-BdAtg8)。BdATG8的靶向敲除阻断了细胞自噬过程,敲除突变体(ΔBdAtg8)的气生菌丝减少且生长速率下降,无性及有性生殖能力完全丧失。此外,ΔBdAtg8对植保素和氧化压力的敏感度显著上升,表明BdATG8在抵御植保素及活性氧(ROS)介导的植物免疫过程中发挥重要作用。苹果枝干及果实致病力测定结果表明,BdATG8缺失突变体的致病力严重下降。综上所述,BdATG8在苹果轮纹病菌的发育、压力响应和致病过程中发挥重要作用。本研究首次在苹果轮纹病菌中系统地研究了BdATG8介导的细胞自噬所调控的生命活动,研究结果有助于全面了解细胞自噬在真核生物中的功能,同时为发掘防治苹果轮纹病的药剂靶标提供了新的思路


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2. Development and application of microsatellite markers within transcription factors in flare tree peony (Paeonia rockii) based on next-generation and single-molecule long-read RNA-seq
LIU Na, CHENG Fang-yun, GUO Xin, ZHONG Yuan
Journal of Integrative Agriculture    2021, 20 (7): 1832-1848.   DOI: 10.1016/S2095-3119(20)63402-5
摘要130)      PDF    收藏

牡丹是起源于中国的具有观赏、药用和食用价值的珍贵作物,其中紫斑牡丹是芍药属中最具代表性的种质之一。EST-SSR标记的开发和应用对于遗传和育种应用具有重要的价值,但芍药属中的EST-SSR资源仍非常有限。在本研究中,我们首先报道了紫斑牡丹中基于下一代(NGS)和单分子长度长(SMLRS)转录组测序的转录因子内SSR标记的开发,从959个与产量相关的候选转录因子内鉴定了包含六种核苷酸重复类型的166个EST-SSR标记,平均每5.83个unigenes包含一个SSR,共有102(61.45 %)对引物在用于RNA-seq的两个品种中产生了扩增产物,其中来自于18个基因家族(AP2、bHLH、HSF等)的58(56.86 %)对引物在连锁作图群体的亲本和8个随机抽选的紫斑牡丹样本中均表现为多态性。通过在37个牡丹样本中的评估,58个 EST-SSR标记表现出了高水平的多态性,PIC值的范围为0.32 到0.91(平均值为0.70),转移性研究表明58对引物在芍药属的7个野生种中的扩增比率为89.66到100%。此外,在62个样本中开展了遗传关系的研究,聚类分析表明主要的聚类和已知的谱系树是基本一致的。综上所述,这些新开发的EST-SSR标记在牡丹种质的保护以及标记辅助选择育种中具有重要的潜在用途。


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3. Straw layer burial to alleviate salt stress in silty loam soils: Impacts of straw forms
ZHANG Hong-yuan, LU Chuang, PANG Huan-cheng, LIU Na, ZHANG Xiao-li, LI Yu-yi
Journal of Integrative Agriculture    2020, 19 (1): 265-276.   DOI: 10.1016/S2095-3119(19)62737-1
摘要131)      PDF    收藏
Salt stress can be alleviated by straw layer burial in the soil, but little is known of the appropriate form of the straw layer for optimal regulation of soil water and salinity because of the uncontrollability of field tests.  Here, the following four straw forms with compaction thickness of 5 cm buried 40–45 deep were studied: no straw layer (CK), segmented straw (SL, 5 cm in length), straw pellet (SK), and straw powder (SF).  The three straw forms (SL, SK and SF) significantly delayed the infiltration of irrigation water down the column profile by 71.20–134.3 h relative to CK and the migration velocity of the wetting front under SF was the slowest.  It took longer for the wetting front to transcend SK than SL but shorter for it to reach the bottom of soil column after water crossed the straw layer.  Compared with CK, the average volumetric water content in the 0–40 cm soil layer increased by 6.45% under SL, 1.77% under SK and 5.39% under SF.  The desalination rates at the 0–40 and 0–100 cm soil layers increased by 5.85 and 3.76% under SL, 6.64 and 1.47% under SK and 5.97 and 4.82% under SF.  However, there was no significant difference among straw forms in the 0–40 cm soil layer.  Furthermore, the salt leaching efficiency (SLE, g mm–1 h–1) above the 40 cm layer under SL was 0.0097, being significantly higher than that under SF (0.0071) by 37.23%.  Salt storage under SL, SK and SF in the 40–45 cm layer accounted for 4.50, 16.92 and 7.43% of total storage in the 1-m column profile.  Cumulative evaporation under SL and SF decreased significantly by 41.20 and 49.00%, with both treatments having the most significant inhibition of salt accumulation (resalinization rate being 36.06 and 47.15% lower than CK) in the 0–40 cm soil layer.  In conclusion, the different forms of straw layers have desalting effects under high irrigation level (446 mm).  In particular, SL and SF performed better than SK in promoting deep salt leaching and inhibiting salt accumulation on the soil surface.  However, SL was simpler to implement and its SLE was higher.  Therefore, the segmented 5 cm straw can be recommended as an optimum physical form for establishing a straw layer for managing saline soils for crop production.
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4. A larval specific OBP able to bind the major female sex pheromone component in Spodoptera exigua (Hübner)
JIN Rong, LIU Nai-yong, LIU Yan, DONG Shuang-lin
Journal of Integrative Agriculture    2015, 14 (7): 1356-1366.   DOI: 10.1016/S2095-3119(14)60849-2
摘要2086)      PDF    收藏
Odorant binding proteins (OBPs) in insects are postulated to solubilize and transport the hydrophobic odorants across the hydrophilic antennal lymph to the olfactory receptors (ORs) located on the dendrite membrane of the sensory neurons. OBPs in adult insects have been intensively reported, but those in larvae are rarely addressed. In our study, a full-length OBP cDNA, namely SexiOBP13, was cloned by RT-PCR and RACE strategy from the heads of Spodoptera exigua larvae. The quantitative real-time PCR (qPCR) measurement indicated that SexiOBP13 was highly expressed in larval head, but very low in other parts of larva and was not detected in any tissues of adult. The binding affinities of SexiOBP13 to plant volatiles and female sex pheromone components were measured by competitive binding assays. Interestingly, SexiOBP13 displayed a high binding affinity (Ki=3.82 μmol L–1) to Z9,E12–14:Ac, the major sex pheromone component of S. exigua, while low affinities to the tested host plant volatiles (Ki>27 μmol L–1). The behavioral tests further confirmed that Z9,E12–14:Ac was indeed active to elicit the behavioral activity of the third instar larvae of S. exigua. Taken together, our results suggest that SexiOBP13 may play a role in reception of female sex pheromone in S. exigua larvae. The ecological significance of the larvae preference to the adult female sex pheromone was discussed.
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5. Actin and Myosin Co-Localize in Plasmodesmata and Ectodesmata-Like Structure
DONG Yu, LIU Na, LIU Gang, LI Wen-long, YAN Ai-hua, WANG Dong-mei
Journal of Integrative Agriculture    2011, 10 (6): 845-849.   DOI: 10.1016/S1671-2927(11)60070-9
摘要3041)      PDF    收藏
Actin and myosin were found to be associated with the cytoplasmic sleeve of plasmodesmata. As cytoskeletal proteins, actin and myosin are believed to regulate the conductivity of plasmodesmata (PDs) in higher plants. Using immunocytochemical methods, we found the two proteins to be co-localized - and closely linked to each other - in plasmodesmata and ectodesmata-like structure in ageing parenchymatous cells of Allium sativum L. We suggest that intercellular communication is affected by the interaction between actin and myosin.
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