细胞自噬相关基因BdATG8参与调控苹果轮纹病菌的发育和致病过程

doi:10.1016/S2095-3119(21)63863-7

Journal of Integrative Agriculture ›› 2022, Vol. 21 ›› Issue (8): 2319-2328.DOI: 10.1016/S2095-3119(21)63863-7

细胞自噬相关基因BdATG8参与调控苹果轮纹病菌的发育和致病过程

收稿日期:2021-08-06 接受日期:2021-11-11 出版日期:2022-08-01 发布日期:2021-11-11

Involvement of the autophagy-related gene BdATG8 in development and pathogenicity in Botryosphaeria dothidea

LIU Na, LIAN Sen, ZHOU Shan-yue, WANG Cai-xia, REN Wei-chao, LI Bao-hua

College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, P.R.China

Received:2021-08-06 Accepted:2021-11-11 Online:2022-08-01 Published:2021-11-11
About author:LIU Na, E-mail: liunalz@163.com; Correspondence REN Wei-chao, E-mail: renweichaoqw@163.com; LI Bao-hua, E-mail: baohuali@qau.edu.cn
Supported by:
This work was supported by the China Agriculture Research System of MOF and MARA (CARS-27), the National Natural Science Foundation of China (32001937) and the Scientific Research Fund for High-level Talents in Qingdao Agricultural University, China (665/1120060).

摘要/Abstract

摘要：

本研究鉴定并研究了苹果轮纹病菌的细胞自噬关键基因BdATG8。研究结果发现，BdATG8能够恢复酿酒酵母ATG8缺失突变体对氮源饥饿的耐受性缺陷。我们建立了检测轮纹病菌细胞自噬过程发生的有效标记（GFP-BdAtg8）。BdATG8的靶向敲除阻断了细胞自噬过程，敲除突变体（ΔBdAtg8）的气生菌丝减少且生长速率下降，无性及有性生殖能力完全丧失。此外，ΔBdAtg8对植保素和氧化压力的敏感度显著上升，表明BdATG8在抵御植保素及活性氧（ROS）介导的植物免疫过程中发挥重要作用。苹果枝干及果实致病力测定结果表明，BdATG8缺失突变体的致病力严重下降。综上所述，BdATG8在苹果轮纹病菌的发育、压力响应和致病过程中发挥重要作用。本研究首次在苹果轮纹病菌中系统地研究了BdATG8介导的细胞自噬所调控的生命活动，研究结果有助于全面了解细胞自噬在真核生物中的功能，同时为发掘防治苹果轮纹病的药剂靶标提供了新的思路。

Abstract:

Botryosphaeria dothidea is a destructive fungal pathogen that causes Botryosphaeria canker and fruit ring rot on apple worldwide. Autophagy is a process of self-degradation that maintains intracellular homeostasis via lysosomal pathway. To date, the biological role of autophagy in B. dothidea remains unknown. In this study, we identified and characterized the autophagy-related gene BdATG8 in B. dothidea. BdATG8 was able to restore the defect in nitrogen starvation tolerance of Saccharomyces cerevisiae ATG8 deletion mutant. GFP-BdAtg8 was shown to be a useful marker for monitoring autophagy in B. dothidea. Target deletion of BdATG8 (ΔBdAtg8) blocked autophagy and significantly impaired mycelial growth, conidiation and perithecium formation. In addition, ΔBdAtg8 showed significantly increased sensitivity to phytoalexin and oxidative stress, suggesting that BdATG8 plays critical roles in overcoming phytoalexin and reactive oxygen species (ROS)-mediated plant immunity. Pathogenicity assays revealed that ΔBdAtg8 almost lost ability to infect hosts. Overall, our results indicate that BdATG8 plays an important role in fungal development, stress responses and pathogenesis in B. dothidea.

Key words: Botryosphaeria dothidea , autophagy , BdATG8 , development , pathogenicity

. 细胞自噬相关基因BdATG8参与调控苹果轮纹病菌的发育和致病过程[J]. Journal of Integrative Agriculture, 2022, 21(8): 2319-2328.

LIU Na, LIAN Sen, ZHOU Shan-yue, WANG Cai-xia, REN Wei-chao, LI Bao-hua. Involvement of the autophagy-related gene BdATG8 in development and pathogenicity in Botryosphaeria dothidea[J]. Journal of Integrative Agriculture, 2022, 21(8): 2319-2328.

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细胞自噬相关基因BdATG8参与调控苹果轮纹病菌的发育和致病过程

Involvement of the autophagy-related gene BdATG8 in development and pathogenicity in Botryosphaeria dothidea

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