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1. Pre-harvest spraying of oxalic acid improves postharvest quality associated with increase in ascorbic acid and regulation of ethanol fermentation in kiwifruit cv. Bruno during storage
Maratab Ali, LIU Meng-meng, WANG Zhen-e, LI Sheng-e, JIANG Tian-jia, ZHENG Xiao-lin
Journal of Integrative Agriculture    2019, 18 (11): 2514-2520.   DOI: 10.1016/S2095-3119(19)62791-7
摘要107)      PDF    收藏
The kiwifruit trees (Actinidia deliciosa cv. Bruno) were sprayed with 5 mmol L–1 oxalic acid (OA) or water (as control) at 130, 137 or 144 d after full-blossom, and then the fruit were harvested at commercial maturity and stored at room temperature (20±1)°C for 13 d.  The effect of pre-harvest spraying of OA on postharvest quality of kiwifruit was evaluated during storage.  The OA spraying slowed the increase in soluble solids content (SSC) and decrease in titratable acid (TA), as well as increased contents of ascorbic acid (AsA) and total-AsA accompanied with higher AsA/DHA ratio in kiwifruit during storage.  Moreover, the OA spraying significantly reduced the contents of acetaldehyde and ethanol in kiwifruit, along with significant decrease in activities of enzymes involved in ethanol fermentation metabolism during the later period of storage, which was beneficial to control off-flavor associated with over accumulation of ethanol during postharvest.  It was suggested that pre-harvest spraying of OA might maintain the postharvest quality of kiwifruit in relation to delay in fruit ripening, AsA maintenance and regulation of ethanol fermentation.
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2. Specific Expression of a Novel Nodulin GmN479 Gene in the Infected Cells of Soybean (Glycine max) Nodules
CHENG Xian-guo, WANG Li, WANG He, YU Guo-hong, BAI You-lu , LIU Meng-meng
Journal of Integrative Agriculture    2011, 10 (10): 1512-1524.   DOI: 10.1016/S1671-2927(11)60146-6
摘要1772)      PDF    收藏
A novel nodulin gene, GmN479 genomic clone composing of 3 630 nucleotides was isolated from mature soybean nodules using GmN479 cDNA as a probe by subtractive hybridization procedure. GmN479 encodes 170 amino acids with 2.09 kb nucleotides promoter region, and contains two important upstream promoter elements, one is a conserved cis-acting sequence motif 5´-AAAGAT-3´ controlling nodulin gene expression, and the other is typical CAAT boxes. GmN479 gene has a single zinc-finger C2H2 domain YSCAFCQRGFSNAQALLGGHMNIH and a conserved motif, QALGGHMN in the zinc-finger with a short leucine repeat in the LDLELRLGL motif closed to C-terminal. These two conserved motifs share respectively higher identity with those in the floral regulator SUPERMAN gene, indicating that GmN479 may function as a transcriptional regulator, and is a likely candidate for playing a role in nodule-morphogenesis. Blotting data showed that GmN479 is a single copy presenting in the genome of soybean nodule, and its expression profile is similar to that of Lba, but it is different from that of ENOD2. GUS staining showed that GmN479 promoter just functions in the infected cells of nodules, indicating that the GmN479 is one of the truly symbiotically induced host genes, and belongs to a late nodulin gene. The expression pattern of GmN479 gene seems to imply that it may be closely related to the development of the nodule. In a sense, it may be a useful marker for identifying the development of the infected cell system in the nodules of soybean.
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