Specific Expression of a Novel Nodulin GmN479 Gene in the Infected Cells of Soybean (Glycine max) Nodules

doi:10.1016/S1671-2927(11)60146-6

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (10): 1512-1524.DOI: 10.1016/S1671-2927(11)60146-6

Specific Expression of a Novel Nodulin GmN479 Gene in the Infected Cells of Soybean (Glycine max) Nodules

CHENG Xian-guo, WANG Li, WANG He, YU Guo-hong, BAI You-lu , LIU Meng-meng

1.Key Laboratory of Crop Nutrition and Fertilizer, Agriculture of Ministry/Insititute of Agricultural Resource and Regional Planning,Chinese Academy of Agricultural Sciences
2.Shanxi Normal University, College of Life Science

收稿日期:2010-06-09 出版日期:2011-10-01 发布日期:2011-10-18
通讯作者: Correspondence CHENG Xian-Guo, Professor, Tel: +86-10-82109745, Fax: +86-10-82106225, E-mail: chengxianguo@tsinghua.org.cn
作者简介:CHENG Xian-Guo, Professor, Tel: +86-10-82109745, Fax: +86-10-82106225, E-mail: chengxianguo@tsinghua.org.cn
基金资助:
This work was supported by the National Key Project for Cultivation of New Varieties of Genetically Modified Organisms (2008ZX08002-005).

Specific Expression of a Novel Nodulin GmN479 Gene in the Infected Cells of Soybean (Glycine max) Nodules

CHENG Xian-guo, WANG Li, WANG He, YU Guo-hong, BAI You-lu , LIU Meng-meng

1.Key Laboratory of Crop Nutrition and Fertilizer, Agriculture of Ministry/Insititute of Agricultural Resource and Regional Planning,Chinese Academy of Agricultural Sciences
2.Shanxi Normal University, College of Life Science

Received:2010-06-09 Online:2011-10-01 Published:2011-10-18
Contact: Correspondence CHENG Xian-Guo, Professor, Tel: +86-10-82109745, Fax: +86-10-82106225, E-mail: chengxianguo@tsinghua.org.cn
About author:CHENG Xian-Guo, Professor, Tel: +86-10-82109745, Fax: +86-10-82106225, E-mail: chengxianguo@tsinghua.org.cn
Supported by:
This work was supported by the National Key Project for Cultivation of New Varieties of Genetically Modified Organisms (2008ZX08002-005).

摘要/Abstract

摘要： A novel nodulin gene, GmN479 genomic clone composing of 3 630 nucleotides was isolated from mature soybean nodules using GmN479 cDNA as a probe by subtractive hybridization procedure. GmN479 encodes 170 amino acids with 2.09 kb nucleotides promoter region, and contains two important upstream promoter elements, one is a conserved cis-acting sequence motif 5´-AAAGAT-3´ controlling nodulin gene expression, and the other is typical CAAT boxes. GmN479 gene has a single zinc-finger C2H2 domain YSCAFCQRGFSNAQALLGGHMNIH and a conserved motif, QALGGHMN in the zinc-finger with a short leucine repeat in the LDLELRLGL motif closed to C-terminal. These two conserved motifs share respectively higher identity with those in the floral regulator SUPERMAN gene, indicating that GmN479 may function as a transcriptional regulator, and is a likely candidate for playing a role in nodule-morphogenesis. Blotting data showed that GmN479 is a single copy presenting in the genome of soybean nodule, and its expression profile is similar to that of Lba, but it is different from that of ENOD2. GUS staining showed that GmN479 promoter just functions in the infected cells of nodules, indicating that the GmN479 is one of the truly symbiotically induced host genes, and belongs to a late nodulin gene. The expression pattern of GmN479 gene seems to imply that it may be closely related to the development of the nodule. In a sense, it may be a useful marker for identifying the development of the infected cell system in the nodules of soybean.

关键词: nodulin, GmN479 promoter, soybean nodule, transgenic plants

Abstract: A novel nodulin gene, GmN479 genomic clone composing of 3 630 nucleotides was isolated from mature soybean nodules using GmN479 cDNA as a probe by subtractive hybridization procedure. GmN479 encodes 170 amino acids with 2.09 kb nucleotides promoter region, and contains two important upstream promoter elements, one is a conserved cis-acting sequence motif 5´-AAAGAT-3´ controlling nodulin gene expression, and the other is typical CAAT boxes. GmN479 gene has a single zinc-finger C2H2 domain YSCAFCQRGFSNAQALLGGHMNIH and a conserved motif, QALGGHMN in the zinc-finger with a short leucine repeat in the LDLELRLGL motif closed to C-terminal. These two conserved motifs share respectively higher identity with those in the floral regulator SUPERMAN gene, indicating that GmN479 may function as a transcriptional regulator, and is a likely candidate for playing a role in nodule-morphogenesis. Blotting data showed that GmN479 is a single copy presenting in the genome of soybean nodule, and its expression profile is similar to that of Lba, but it is different from that of ENOD2. GUS staining showed that GmN479 promoter just functions in the infected cells of nodules, indicating that the GmN479 is one of the truly symbiotically induced host genes, and belongs to a late nodulin gene. The expression pattern of GmN479 gene seems to imply that it may be closely related to the development of the nodule. In a sense, it may be a useful marker for identifying the development of the infected cell system in the nodules of soybean.

Key words: nodulin, GmN479 promoter, soybean nodule, transgenic plants

CHENG Xian-guo, WANG Li, WANG He, YU Guo-hong, BAI You-lu , LIU Meng-meng. Specific Expression of a Novel Nodulin GmN479 Gene in the Infected Cells of Soybean (Glycine max) Nodules[J]. Journal of Integrative Agriculture, 2011, 10(10): 1512-1524.

参考文献

[1]An G, Ebert P R, Mitra A, Ha S B. 1988. Binary vectors. In: Gelvin S B, Schilperoort R A, eds., Plant Molecular Biology Manual. Kluwer Academic in Dordrecht, London. pp. 1-19.

[2]Berg J M. 1986. Potential metal-binding domain in nucleic acid binding proteins. Science, 232, 485-487.

[3]Bouhouche N, Syvanen M, Kado C I. 2000. The origin of prokaryotic C2H2 zinc finger regulators. Trends in Micrbiology, 8, 77-81.

[4]Brown R S. 2005. Zinc finger proteins: getting a grip on RNA. Current Opinion in Structural Biology, 15, 94-98.

[5]de Bruijin F J, Szabados L, Schell J. 1990. Chimeric genes and transgenic plants are used to study the regulation of genes involved in symbiotic plant-microbe interactions. Developmental Genetics, 11, 182-196.

[6]Cheng X G, Nomura M, Takane K, Kouchi H, Tajima S. 2000. Expression of two uricase (nodulin-35) genes in a non-ureide type legume alfalfa, Medicago sativa. Plant Cell Physiology, 41, 104-109.

[7]Chrispeels H E, Oettinger H, Janvier N,Tague B W. 2000. AtZFP1, encoding Arabidopsis thaliana C2H2 zinc-finger protein1, is expressed downstream of photomorphogenic activation. Plant Molecular Biology, 42, 279-290.

[8]Fang Y, Hirsch A M. 1998. Studying early nodulin gene ENOD40 expression and induction by nodulation factor and cytokinin in transgenic alfalfa. Plant Physiology, 116, 53-68.

[9]Franssen H J, Thompson D V, Idler K, Kormelink R, van Kammen A, Bisseling T. 1990. Nucleotide sequence of two soybean ENOD2 early nodulin genes encoding Ngm-75. Plant Molecular Biology, 14, 103-106.

[10]Furner I, Huffman G, Amasino R M, Garfinkel D J, Gordon M P, Nester E W. 1986. An Agrobacterium transformation in the evolution of the genus Nicotiana. Nature, 319, 422-427.

[11]Gamas P, Niebel F D C, Lescure N, Cullimore J V. 1996. Use of a subtractive hybridization approach to identify new Medicago truncatula genes induced during root nodule development. Molecular Plant-Microbe Interactions, 9, 233- 242.

[12]Ganss B, Jheon A. 2004. Zinc finger transcription factors in skeletel development. Critical Reviews in Oral Biology & Medicine, 15, 282-297.

[13]Gresshoff P M. 2003. Post-genomic insights into plant nodulation symbioses. Genome Biology, 4, 201-205.

[14]Guenther J F, Chanmanivone N, Galetovic M P, Wallace I S, Cobb J A, Roberts D M. 2003. Phosphorylation of soybean nodulin 26 on serine 262 enhances water permeability and is regulated developmentally and by osmotic signals. The Plant Cell, 115, 981-991.

[15]Ito T, Sakai H, Meyerowitz E M. 2003. Whorl-specific expression of the SUPERMAN gene of Arabidopsis is mediated by cis elements in the transcribed region. Current Biology, 13, 1524-1530.

[16]Jeffersen R A, Kavanagh T A, Bevan M W. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO Journal, 6, 3901-3907.

[17]Jesen E, Marker K A, Schell J, de Bruijin F J. 1988. Interaction of a nodule specific, trans-acting factor with distinct DNA elements in the soybean leghemoglobin Lbc3 5´ upstream region. The EMBO Journal, 7, 1265-1271.

[18]Jorgensen J E, Stougaard J, Marker K A. 1991. A two-component nodule-specific enhancer in the soybean N23 gene promoter. The Plant Cell, 3, 819-827.

[19]Kapranov P, de Bruijn F J, Szczyglowski K. 1997. Novel, highly expressed late nodulin gene (LjNOD16) from Lotus japonicus. Plant Physiology, 113, 1081-1090.

[20]Kouchi H, Hata S. 1995. GmN56, a novel nodule-specific cDNA from soybean root nodules encodes a protein homologous to isopropylmalate synthase and homocitrate synthase. Molecular Plant-Microbe Interactions, 8, 172-176.

[21]Kouchi H, Shimomura K, Hata S, Hirota A, Wu G J, Kumagai H, Tajima S, Suganuma N, Suzuki A, Aoki T, et al. 2004. Largescale analysis of gene expression profiles during early stages of root nodule formation in a model legume. Lotus japonicus. DNA Research, 11, 263-274.

[22]Laporte P, Satiat-Jeunemaître B, Velasco I, Csorba T, van de Velde W, Campalans A, Burgyan J, Arevalo-Rodriguez M, Crespi M. 2009. A novel RNA-binding peptide regulates the establishment of the Medicago truncatula-Sinorhizobium meliloti nitrogen-fixing symbiosis. The Plant Journal, 62, 24-38.

[23]Lee H, Hur C G, Oh C J, Kim H B, Pakr S Y, An C S. 2004. Analysis of the root nodule-enhanced transcriptome in soybean. Molecules and Cells, 18, 53-62.

[24]Liu Q P, Wang H S, Zhang Z H, Wu J S, Feng Y, Zhu Z J. 2009. Divergence in function and expression of the NOD26-like intrinsic proteins in plants. Genomics, 10, 313-325.

[25]Macknight R C, Reynolds P H, Farnden K J. 1995. Analysis of the lupin nodulin-45 promoter: conserved regulatory sequences are important for promoter activity. Plant Molecular Biology, 7, 457-466.

[26]Maniatis T, Goodbourn S, Fischer J. 1987. Regulation of inducible and tissue-specific gene expression. Science, 236, 1237-1245.

[27]Murray J D, Karas B J, Sato S, Tabata S, Amyot L, Szczyglowski K. 2007. A cytokinin perception mutant colonized by Rhizobium in the absence of nodule organogenesis. Science, 315, 101-104.

[28]Nap J P, Bisseling T. 1990. Development biology of a plant prokaryotes symbiosis: The legume root nodule. Science, 250, 948-954.

[29]Nielsen A L, Jørgensen P, Lerouge T, Cerviño M, Chambon P, Losson R. 2004. Nizp1, a novel multitype zinc finger protein that interacts with the NSD1 histone lysine methyltransferase through a unique C2HR motif. Molecular Cell Biology, 24, 5184-5196.

[30]OW D W,Wood K V, Deluca M, Wet J R, Howell S H. 1986. Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science, 234, 856-859.

[31]Petit A, Stougaard J, Kuhle A, Marker K A, Temple J. 1987. Transformation and regeneration of the legume Lotus corniculatus: A system for molecular studies of symbiotic nitrogen fixation. Molecular Genetics and Genomics, 207, 245-250.

[32]Sakai H, Medrano L J, Elliot M. 1995. Role of SUPERMAN in maintaining Arabidopsis floral whorl boundaries. Nature, 378, 199-203.

[33]Sambrook J, Fritsch E F, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, New York. Sanger F, Nicklen S, Coulson A R. 1977. DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the USA, 74, 5463-5467.

[34]Schultze M, Kondorosi A. 1998. Regulation of symbiotic root nodule development. Annual Review of Genetics, 32, 33-57.

[35]She Q X, Sandal N N, Stougaard J, Marker K A. 1993. Comparative sequence analysis of cis elements present in Glycine max L. leghemoglobin Lba and lbc3 genes. Plant Molecular Biology, 22, 931-935.

[36]Stougaard J, Jorgensen J E, Christensen T, Kuhle A, Marker K A. 1990. Independence and nodule specificity of cis-acting regulatory elements in the soybean leghemoglobin lbc3 and N23 genes promoters. Molecular Genetics and Genomics, 220, 353-360.

[37]Szczyglowski K, Potter T, Stoltzfus J, Fujimoto S Y, de Bruijin F J. 1996. Differential expression of the Sesbania rostrata leghemoglobin glb3 gene promoter in transgenic legume and non-legume plants. Plant Molecular Biology, 31, 931-935.

[38]Tajima S,Yamamoto Y. 1977. Regulation of uricase activity in developing roots of Glycine max, non-nodulating variety A62- 2.

[39]Plant Cell Physiology, 18, 247-253.

[40]Takatsuji H. 1999. Zinc-finger proteins: the classical zinc finger emerges in contemporary plant science. Plant Molecular Biology, 39, 1073-1078.

[41]Takatsuji H, Nakamura N, Katsumoto Y. 1994. A new family of zinc finger proteins in petunia: structure DNA sequences recognition, and floral organ-specific expression. The Plant Cell, 6, 947-958.

[42]Terada R, Ignacimuthu S, Bauer P, Kondorosi E, Schultze M, Kondorosi A, Potrykus I, Sautter C. 2001. Expression of early nodulin promoter gene in transgenic rice. Current Science, 81, 270-276.

[43]Thérèse T L, Elisa C, Michèle C, Guilhem D, Valérie H, Bertrand H A. 1999. A strong constitutive positive element is essential for the ammonium-regulated expression of a soybean gene encoding cytosolic glutamine synthtase. Plant Molecular Biology, 39, 551-564.

[44]Trevaskis B, Wandrey M, Colebatch G, Udvardi M K. 2002. The soybean GmN6L gene encodes a late nodulin expressed in the infected zone of nitrogen-fixing nodules. Molecular Plant-Microbe Interactions, 15, 630-636.

[45]Vincill E D, Szczyglowski K, Roberts D M. 2005. GmN70 and LjN70 are nodule anion transporters of the symbiosome membrane of nodules with a transport preference for nitrate. Plant Physiology, 137, 1435-1444.

[46]Wang D, Griffitts J, Starker C, Fedorova E, Limpens E, Ivanov S, Bisseling T, Long L. 2010. A nodule-specific proteinsecretory pathway required for nitrogen-fixing symbiosis. Science, 327, 1126-1129.

[47]Wang Y Z, Yu G Q, Shen S J, Zhu J B. 2004. Promoter of soybean early nodulin gene enod2B is induced by rhizobial nod factors in transgenic rice. Chinese Science Bulletin, 49, 343-349.

[48]Wycoff K L, Hunt S, Gonzales M B, VandenBosch K A, Layzell D B, Hirsch A M. 1998. Effects of oxygen on nodule physiology and expression of nodulins in alfalfa. Plant Physiology, 117, 385-395.

Specific Expression of a Novel Nodulin GmN479 Gene in the Infected Cells of Soybean (Glycine max) Nodules

Specific Expression of a Novel Nodulin GmN479 Gene in the Infected Cells of Soybean (Glycine max) Nodules

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