在高等植物中，茎尖分生组织以规则的间隔（叶序）和时间（叶间期）生成侧生器官。对叶序和叶间期相关突变体分析将加深对植物茎尖结构发育机制的理解。本研究中，扬农啤5号EMS诱变获得一个出叶速率变快、节间数目增多和矮化突变体，命名为mnd8^ynp5。利用图位克隆的方法，mnd8基因定位于5H染色体长臂6.7kb的基因组区间。序列分析表明，HORVU5Hr1G118820在第一个外显子953位发生了C到T单核苷酸突变，导致编码蛋白第318位氨基酸由丙氨酸（Ala）变为缬氨酸（Val）。HORVU5Hr1G118820作为MND8基因的候选基因，编码514个氨基酸，包含两个多药和有毒化合物挤压结构域（MATE），与玉米Bige1高度同源，具有通过控制叶片起始速率来调控植物发育的保守功能。现代大麦品种单倍型分析表明，Hap-1是被全世界大麦育种中应用的主要单倍型。总之，mnd8^ynp5作为HORVU5Hr1G118820基因的新等位基因，调控了大麦叶间期和矮秆多节表型。

Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of expression, proteomic profiles in maize seedling leaves were analyzed by high-throughput two-dimensional gel electrophoresis, combined with MALDI-TOF MS technology. Of the 464 proteins that were detected with silver staining in a pH range of 4-7, 17 (3.66%) showed clock rhythmicity in their abundance. These proteins belonged to diverse functional groups and proteins involved in photosynthesis and carbon metabolism were over-represented. These findings provide a new perspective on the relationship between the physiological functions of leaves and the clock rhythmic system.