MATE转运蛋白基因中单核苷酸的替换调控了大麦叶隔期和矮秆多节表型

doi:10.1016/j.jia.2023.02.006

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (8): 2295-2305.DOI: 10.1016/j.jia.2023.02.006

MATE转运蛋白基因中单核苷酸的替换调控了大麦叶隔期和矮秆多节表型

收稿日期:2022-08-28 接受日期:2022-11-10 出版日期:2023-08-20 发布日期:2023-08-04

A single nucleotide substitution in the MATE transporter gene regulates plastochron and many noded dwarf phenotype in barley (Hordeum vulgare L.)

GUO Bao-jian^{1, 2*}, SUN Hong-wei^1*, QI Jiang¹, HUANG Xin-yu¹, HONG Yi¹, HOU Jian¹, LÜ Chao¹, WANG Yu-lin², WANG Fei-fei¹, ZHU Juan¹, GUO Gang-gang³, XU Ru-gen¹

¹ Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics, Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, P.R.China
² State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement/Tibet Academy of Agricultural and Animal Husbandry Sciences (TAAAS), Lhasa 850002, P.R.China
³ Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture/National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

Received:2022-08-28 Accepted:2022-11-10 Online:2023-08-20 Published:2023-08-04
About author:GUO Bao-jian, E-mail: bjguo@yzu.edu.cn; SUN Hong-wei, E-mail: 2939067986@qq.com; #Correspondence XU Ru-gen, Tel: +86-514-87979254, E-mail: rgxu@yzu.edu.cn * These authors contributed equally to this study.
Supported by:
This work was funded by the Open Project Program of State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement, China (XZNKY-2021-C-014-K01), the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (19KJA560005), the China Agriculture Research System (CARS-05), and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China.

摘要/Abstract

摘要：

在高等植物中，茎尖分生组织以规则的间隔（叶序）和时间（叶间期）生成侧生器官。对叶序和叶间期相关突变体分析将加深对植物茎尖结构发育机制的理解。本研究中，扬农啤5号EMS诱变获得一个出叶速率变快、节间数目增多和矮化突变体，命名为mnd8^ynp5。利用图位克隆的方法，mnd8基因定位于5H染色体长臂6.7kb的基因组区间。序列分析表明，HORVU5Hr1G118820在第一个外显子953位发生了C到T单核苷酸突变，导致编码蛋白第318位氨基酸由丙氨酸（Ala）变为缬氨酸（Val）。HORVU5Hr1G118820作为MND8基因的候选基因，编码514个氨基酸，包含两个多药和有毒化合物挤压结构域（MATE），与玉米Bige1高度同源，具有通过控制叶片起始速率来调控植物发育的保守功能。现代大麦品种单倍型分析表明，Hap-1是被全世界大麦育种中应用的主要单倍型。总之，mnd8^ynp5作为HORVU5Hr1G118820基因的新等位基因，调控了大麦叶间期和矮秆多节表型。

Abstract: In higher plants, the shoot apical meristem produces lateral organs in a regular spacing (phyllotaxy) and timing (plastochron). The molecular analysis of mutants associated with phyllotaxy and plastochron would increase our understanding of the mechanism of shoot architecture formation. In this study, we identified mutant mnd8^ynp5 that shows an increased rate of leaf emergence and a larger number of nodes in combination with a dwarfed growth habit from an EMS-treated population of the elite barley cultivar Yangnongpi 5. Using a map-based cloning strategy, the mnd8 gene was narrowed down to a 6.7-kb genomic interval on the long arm of chromosome 5H. Sequence analysis revealed that a C to T single-nucleotide mutation occurred at the first exon (position 953) of HORVU5Hr1G118820, leading to an alanine (Ala) to valine (Val) substitution at the 318th amino acid site. Next, HORVU5Hr1G118820 was defined as the candidate gene of MND8 encoding 514 amino acids and containing two multidrug and toxic compound extrusion (MATE) domains. It is highly homologous to maize Bige1 and has a conserved function in the regulation of plant development by controlling the leaf initiation rate. Examination of modern barely varieties showed that Hap-1 was the dominant haplotype and was selected in barley breeding around the world. Collectively, our results indicated that mnd8^ynp5 is a novel allele of the HORVU5Hr1G118820 gene that is possibly responsible for the shortened plastochron and many noded dwarf phenotype in barley.

Key words: barley , EMS , plastochron , many noded dwarf , MATE transporter , shoot architecture

GUO Bao-jian, SUN Hong-wei, QI Jiang, HUANG Xin-yu, HONG Yi, HOU Jian, LÜ Chao, WANG Yu-lin, WANG Fei-fei, ZHU Juan, GUO Gang-gang, XU Ru-gen. MATE转运蛋白基因中单核苷酸的替换调控了大麦叶隔期和矮秆多节表型[J]. Journal of Integrative Agriculture, 2023, 22(8): 2295-2305.

GUO Bao-jian, SUN Hong-wei, QI Jiang, HUANG Xin-yu, HONG Yi, HOU Jian, LÜ Chao, WANG Yu-lin, WANG Fei-fei, ZHU Juan, GUO Gang-gang, XU Ru-gen. A single nucleotide substitution in the MATE transporter gene regulates plastochron and many noded dwarf phenotype in barley (Hordeum vulgare L.)[J]. Journal of Integrative Agriculture, 2023, 22(8): 2295-2305.

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MATE转运蛋白基因中单核苷酸的替换调控了大麦叶隔期和矮秆多节表型

A single nucleotide substitution in the MATE transporter gene regulates plastochron and many noded dwarf phenotype in barley (Hordeum vulgare L.)

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