Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (12): 1958-1967.DOI: 10.1016/S1671-2927(00)8732

• 论文 • 上一篇    下一篇

Proteomic Identification of Rhythmic Proteins in Maize Seedling Leaves

 FENG Wan-jun, GUO Bao-jian, YAO Ying-yin, PENGHui-ru, SUN Qi-xin, NI Zhong-fu   

  1. 1.Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/State Key Laboratory for Agrobiotechnology, Ministry of Education/Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
    2.National Plant Gene Research Centre, Beijing 100193, P.R.China
  • 收稿日期:2011-08-16 出版日期:2012-12-01 发布日期:2012-12-18
  • 通讯作者: Correspondence SUN Qi-xin, Tel/Fax: +86-10-62733426, E-mail: qxsun@cau.edu.cn; NI Zhong-fu, Tel/Fax: +86-10-62733426, E-mail: wheat3392@cau.edu.cn
  • 基金资助:

    This work was financially supported by the National Basic Research Program of China (2007CB109000), the National Science Found for Distinguished Young Scholars, China (30925023), the National Natural Science Foundation of China (30671297), and the National High- Tech R&D Program of China (2009AA101102).

Proteomic Identification of Rhythmic Proteins in Maize Seedling Leaves

 FENG Wan-jun, GUO Bao-jian, YAO Ying-yin, PENGHui-ru, SUN Qi-xin, NI Zhong-fu   

  1. 1.Key Laboratory of Crop Heterosis and Utilization, Ministry of Education/State Key Laboratory for Agrobiotechnology, Ministry of Education/Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
    2.National Plant Gene Research Centre, Beijing 100193, P.R.China
  • Received:2011-08-16 Online:2012-12-01 Published:2012-12-18
  • Contact: Correspondence SUN Qi-xin, Tel/Fax: +86-10-62733426, E-mail: qxsun@cau.edu.cn; NI Zhong-fu, Tel/Fax: +86-10-62733426, E-mail: wheat3392@cau.edu.cn
  • Supported by:

    This work was financially supported by the National Basic Research Program of China (2007CB109000), the National Science Found for Distinguished Young Scholars, China (30925023), the National Natural Science Foundation of China (30671297), and the National High- Tech R&D Program of China (2009AA101102).

摘要: Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of expression, proteomic profiles in maize seedling leaves were analyzed by high-throughput two-dimensional gel electrophoresis, combined with MALDI-TOF MS technology. Of the 464 proteins that were detected with silver staining in a pH range of 4-7, 17 (3.66%) showed clock rhythmicity in their abundance. These proteins belonged to diverse functional groups and proteins involved in photosynthesis and carbon metabolism were over-represented. These findings provide a new perspective on the relationship between the physiological functions of leaves and the clock rhythmic system.

关键词: proteomics, rhythmic proteins, maize leaves, mass spectrometry, two-dimensional gel electrophoresis

Abstract: Plant leaves respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24 h period. To determine which proteins exhibited a rhythmic pattern of expression, proteomic profiles in maize seedling leaves were analyzed by high-throughput two-dimensional gel electrophoresis, combined with MALDI-TOF MS technology. Of the 464 proteins that were detected with silver staining in a pH range of 4-7, 17 (3.66%) showed clock rhythmicity in their abundance. These proteins belonged to diverse functional groups and proteins involved in photosynthesis and carbon metabolism were over-represented. These findings provide a new perspective on the relationship between the physiological functions of leaves and the clock rhythmic system.

Key words: proteomics, rhythmic proteins, maize leaves, mass spectrometry, two-dimensional gel electrophoresis