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1. Application of methyl jasmonate postharvest maintains the quality of Nanguo pears by regulating mitochondrial energy metabolism
LI Can-ying, CHENG Yuan, HOU Jia-bao, ZHU Jie, SUN Lei, GE Yong-hong
Journal of Integrative Agriculture    2021, 20 (11): 3075-3083.   DOI: 10.1016/S2095-3119(21)63611-0
摘要156)      PDF    收藏

研究了采后茉莉酸甲酯(MeJA)浸泡处理对常温贮藏期间南果梨果实线粒体能量代谢和贮藏品质的影响。结果表明,采后MeJA处理抑制了果实呼吸速率和失重率的增加,保持了较高的果肉硬度,抗坏血酸含量和可滴定酸。MeJA处理还显著提高了线粒体H+-ATPaseCa2+-ATPase、琥珀酸脱氢酶和细胞色素C氧化酶活性,ATPADP含量及能荷水平。由此说明,采后MeJA处理通过调控线粒体能量代谢来保持南果梨果实常温贮藏品质。

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2. Effects of thiamine on Trichothecium and Alternaria rots of muskmelon fruit and the possible mechanisms involved
GE Yong-hong, LI Can-ying, Lü Jing-yi, ZHU Dan-shi
Journal of Integrative Agriculture    2017, 16 (11): 2623-2631.   DOI: 10.1016/S2095-3119(16)61584-8
摘要789)      PDF    收藏
The effects of thiamine against pink and black spot rots caused by Trichothecium roseum and Alternaria alternata and modulation on the metabolism of reactive oxygen species (ROS) and phenylpropanoid pathway were investigated in this paper.  In vitro test indicated that thiamine significantly inhibited mycelia growth and spore germination of T. roseum and A. alternata.  Thiamine at 100 mmol L–1 effectively inhibited lesion development of muskmelon fruit inoculated with T. roseum or A. alternata, enhanced production rate of O2-. and H2O2 content, activities of catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) in muskmelon fruit.  Thiamine also affect phenylpropanoid pathway in muskmelon fruit by increasing the activities of phenylalanine ammonia lyase (PAL) and peroxidase (POD), the content of total phenolic compounds, flavonoids and lignin.  These results suggest that the effects of thiamine on pink and black spot rots in muskmelon fruits are associated with its direct fungitoxic against the pathogens and the modulation of O2-. and H2O2 production, eliminating enzymes and phenylpropanoid pathway.
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3. Solvent Effects on the Ultrastructure and Chemical Composition of Cuticular Wax and Its Potential Bioactive Role Against Alternaria alternata in Pingguoli Pear
CHEN Song-jiang, LI Yong-cai, BI Yang, YIN Yan, GE Yong-hong , WANG Yi
Journal of Integrative Agriculture    2014, 13 (5): 1137-1145.   DOI: 10.1016/S2095-3119(13)60374-3
摘要2067)      PDF    收藏
Effects of different polarity solvents on the ultrastructure and chemical composition of cuticular wax in Pingguoli pear as well as their bioactive role against Alternaria alternate were studied and the results showed that the highest wax content was extracted with chloroform, and its wax content was up to 322.2 μg cm-2. Long-chain fatty acids predominated in menthol extracts and n-alkanes were predominant in wax extracted with ether, chloroform and n-hexane. Pingguoli pear fruit surface was covered by a smooth and amorphous wax layer with small, scattered crystal. The morphology of recrystallized wax in vitro after removal with different solvents was not similar to that of the intact fruit surface. Removal of cuticular wax with various solvents significantly enhanced A. alternata infection, except for wax removed by methanol. The solvent extracts of methanol and chloroform stimulated the spore germination and mycelium growth of A. alternata, but the ether and n-hexane extracts showed antifungal activity.
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