Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (5): 1137-1145.DOI: 10.1016/S2095-3119(13)60374-3

• 论文 • 上一篇    下一篇

Solvent Effects on the Ultrastructure and Chemical Composition of Cuticular Wax and Its Potential Bioactive Role Against Alternaria alternata in Pingguoli Pear

 CHEN Song-jiang, LI Yong-cai, BI Yang, YIN Yan, GE Yong-hong , WANG Yi   

  1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, P.R.China
  • 收稿日期:2012-11-20 出版日期:2014-05-01 发布日期:2014-05-06
  • 通讯作者: LI Yong-cai, Tel: +86-931-7632256, Fax: +86-931-7631201, E-mail: liyongcai@gsau.edu.cn
  • 作者简介:CHENG Song-jiang, E-mail: 409770766@qq.com
  • 基金资助:

    This research was financially supported by the National Natural Sciences Foundation of China (30960243 and 31260494), and the Fuxi Distinguished Young Talent Cultivation Project of Gansu Agricultural University, China.

Solvent Effects on the Ultrastructure and Chemical Composition of Cuticular Wax and Its Potential Bioactive Role Against Alternaria alternata in Pingguoli Pear

 CHEN Song-jiang, LI Yong-cai, BI Yang, YIN Yan, GE Yong-hong , WANG Yi   

  1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, P.R.China
  • Received:2012-11-20 Online:2014-05-01 Published:2014-05-06
  • Contact: LI Yong-cai, Tel: +86-931-7632256, Fax: +86-931-7631201, E-mail: liyongcai@gsau.edu.cn
  • About author:CHENG Song-jiang, E-mail: 409770766@qq.com
  • Supported by:

    This research was financially supported by the National Natural Sciences Foundation of China (30960243 and 31260494), and the Fuxi Distinguished Young Talent Cultivation Project of Gansu Agricultural University, China.

摘要: Effects of different polarity solvents on the ultrastructure and chemical composition of cuticular wax in Pingguoli pear as well as their bioactive role against Alternaria alternate were studied and the results showed that the highest wax content was extracted with chloroform, and its wax content was up to 322.2 μg cm-2. Long-chain fatty acids predominated in menthol extracts and n-alkanes were predominant in wax extracted with ether, chloroform and n-hexane. Pingguoli pear fruit surface was covered by a smooth and amorphous wax layer with small, scattered crystal. The morphology of recrystallized wax in vitro after removal with different solvents was not similar to that of the intact fruit surface. Removal of cuticular wax with various solvents significantly enhanced A. alternata infection, except for wax removed by methanol. The solvent extracts of methanol and chloroform stimulated the spore germination and mycelium growth of A. alternata, but the ether and n-hexane extracts showed antifungal activity.

关键词: fruit cuticular membrane , Alternaria alternata , antifungal or stimulus activity

Abstract: Effects of different polarity solvents on the ultrastructure and chemical composition of cuticular wax in Pingguoli pear as well as their bioactive role against Alternaria alternate were studied and the results showed that the highest wax content was extracted with chloroform, and its wax content was up to 322.2 μg cm-2. Long-chain fatty acids predominated in menthol extracts and n-alkanes were predominant in wax extracted with ether, chloroform and n-hexane. Pingguoli pear fruit surface was covered by a smooth and amorphous wax layer with small, scattered crystal. The morphology of recrystallized wax in vitro after removal with different solvents was not similar to that of the intact fruit surface. Removal of cuticular wax with various solvents significantly enhanced A. alternata infection, except for wax removed by methanol. The solvent extracts of methanol and chloroform stimulated the spore germination and mycelium growth of A. alternata, but the ether and n-hexane extracts showed antifungal activity.

Key words: fruit cuticular membrane , Alternaria alternata , antifungal or stimulus activity