ABCC2基因是多种鳞翅目昆虫的Bt受体，有研究表明对Cry1F具有抗性的草地贪夜蛾田间种群与ABCC2突变遗传连锁，为探明SfABCC2突变是否介导Cry1F抗性，我们采用CRISPR/Cas9基因编辑技术对草地贪夜蛾ABCC2基因进行敲除。对筛选得到的纯合敲除品系进行生物测定，结果显示Cry1F毒素对敲除品系的LC50为39.2 (29.2–54.2) μg cm^–2，是野生品系的118倍。本研究通过反向遗传学手段证实SfABCC2是Cry1F受体。

Application of fertilizer has been found to significantly affect soil N cycling.  However, a comprehensive understanding of the effects of long-term fertilization on soil gross N transformation rates is still lacking.  We compiled data of observations from 10 long-term fertilization experiments and conducted a meta-analysis of the effects of long-term fertilization on soil gross N transformation rates.  The results showed that if chemical fertilizers of N, P and K were applied in balance, soil pH decreased very slightly.  There was a significantly positive effect of long-term fertilization, either chemical or organic fertilizers or their combinations, on gross N mineralization rate compared to the control treatment (the mean effect size ranged from 1.21 to 1.25 at 95% confidence intervals (CI) with a mean of 1.23), mainly due to the increasing soil total N content.  The long-term application of organic fertilizer alone and combining organic and chemical fertilizer could increase the mineralization-immobilization turnover, thus enhance available N for plant while reduce N losses potential compared to the control treatment.  However, long-term chemical fertilizer application did not significantly affect the gross NH4+ immobilization rate, but accelerated gross nitrification rate (1.19; 95% CI: 1.08 to 1.31).  Thus, long-term chemical fertilizer alone would probably induce higher N losses potential through NO3– leaching and runoff than organic fertilizer application compared to the control treatment.  Therefore, in the view of the effects of long-term fertilization on gross N transformation rates, it also supports that organic fertilizer alone or combination of organic and chemical fertilizer could not only improve crop yield, but also increase soil fertility and reduce the N losses potential.

Improving the yield of maize grain per unit area is needed to meet the growing demand for it in China, where the availability of fertile land is very limited. Modified fertilization management and planting density are efficient methods for increasing crop yield. Field experiments were designed to investigate the influence of modified fertilization management and planting density on grain yield and nitrogen use efficiency of the popular maize variety Zhengdan 958, in four treatments including local farmer’s practice (FP), high-yielding and high efficiency cultivation (HH), super high-yielding cultivation (SH), and the control (CK). Trials were conducted in three locations of the Huang-Huai-Hai Plain in northern China. Compared with FP, SH was clearly able to promote N absorption and dry matter accumulation in post-anthesis, and achieve high yield and N use efficiency by increasing planting density and postponing the supplementary application of fertilizers. However, with an increase in planting density, the demand of N increased along with grain yield. Due to the input of too much N fertilizer, the efficiency of N use in SH was low. Applying less total N, ameliorating cultivation and cropping management practices should be considered as priority strategies to augment production potential and finally achieve synchronization between high yield and high N efficiency in fertile soils. However, in situations where soil fertility is low, achieving high yield and high N use efficiency in maize will likely depend on increased planting density and appropriate application of supplementary fertilizers postpone to the grain-filling stage.