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1. 时序转录组分析揭示了西瓜对低温胁迫的响应
Jin Wang, Minghua Wei, Haiyan Wang, Changjuan Mo, Yingchun Zhu, Qiusheng Kong
Journal of Integrative Agriculture    2025, 24 (5): 1786-1799.   DOI: 10.1016/j.jia.2024.11.025
摘要34)      PDF    收藏

西瓜Citrullus lanatus是一种重要的园艺作物,但其易受低温胁迫的影响,这对西瓜生产和供应提出了重大挑战。尽管西瓜具有重要的经济价值,但对其在转录水平上对低温胁迫的响应知之甚少。在本研究中,我们进行了一个时序转录组分析,系统地研究了西瓜在低温胁迫下的调控网络。共鉴定出6个低温响应基因簇,代表6种表达模式,揭示了低温胁迫下西瓜代谢途径的多样性调控。对时间特异性差异表达基因的分析揭示了西瓜对低温响应的时间依赖性。此外,ClMYB14-OE过表达株系更易受到低温胁迫的影响,因此ClMYB14被发现是低温耐受性的负调控因子。共表达网络分析表明,ClMYB14通过调控不饱和脂肪酸途径和热激转录因子参与低温响应。本研究为了解西瓜响应低温胁迫的调控网络提供了重要信息,并为提高西瓜耐低温性的遗传改良提供了候选基因。

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2.

生物炭优化玉米水分利用效率的调控机制

Heng Wan, Zhenhua Wei, Chunshuo Liu, Xin Yang, Yaosheng Wang, Fulai Liu
Journal of Integrative Agriculture    2025, 24 (1): 132-146.   DOI: 10.1016/j.jia.2024.03.073
摘要53)      PDF    收藏

玉米是干旱和半干旱地区的重要粮食作物,而在水资源有限的种植环境中,提升玉米水分利用效率(WUE)尤为关键。植物生长调控措施对玉米的形态、气孔行为及水分管理有显著影响,但具体机制仍待深入探索。该研究发以传统充分灌溉为对照,揭示了生物炭联合交替灌溉处理在减少玉米水分使用的同时改变了木质部的离子成分,调控了玉米的脱落酸(ABA)信号传导,通过减少气孔开度来降低水分散失,从而显著提高了玉米的水分利用效率(WUE)。研究表明,小麦秸秆生物炭(WSB)在干旱环境中通过改变叶片气孔的大小和密度,增强了植株对水分的高效利用。生物炭联合交替灌溉通过增强ABA信号调节玉米气孔开闭,进而提高了玉米的整体光合效率和抗旱能力。该研究为干旱地区玉米生产中生物炭的应用提供了理论依据,为玉米在水分有限环境下的高效种植提供了新思路。

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3. 水稻分蘖角度自然变异基因BTA8的鉴定
Junrong Liu, Xingyu Wang, Jing Wang, Junhua Ye, Mengchen Zhang, Qun Xu, Yaolong Yang, Xinghua Wei, Baoyan Jia, Yue Feng
Journal of Integrative Agriculture    2024, 23 (8): 2868-2871.   DOI: 10.1016/j.jia.2024.04.008
摘要144)      PDF    收藏
理想株型在水稻品种改良中起到了重要作用。分蘖角度是影响水稻株型的核心要素之一。在此,我们报道了一个位于8号染色体调控水稻分蘖角度的基因BTA8。功能互补实验表明,小粒野生稻BTA8等位基因在日本晴背景下增加了分蘖角度。BTA8在各组织中呈组成型表达,其编码蛋白定位于细胞核和细胞质中。全基因组关联分析显示BTA8基因受到选择,且在调控水稻分蘖角度中发挥关键作用。BTA8-Hap1Hap4单倍型分别在粳稻和籼稻中被选择。研究结果为更好地理解BTA8调控水稻分蘖角度的作用提供了新见解,并为水稻株型改良提供了潜在的靶点。
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4. 一个控制油菜半矮杆性状的主效新位点BnaSD.C3的精细定位及候选基因分析
WANG Xiao-dong, CAI Ying, PANG Cheng-ke, ZHAO Xiao-zhen, SHI Rui, LIU Hong-fang, CHEN Feng, ZHANG Wei, FU San-xiong, HU Mao-long, HUA Wei, ZHENG Ming, ZHANG Jie-fu
Journal of Integrative Agriculture    2023, 22 (10): 2981-2992.   DOI: 10.1016/j.jia.2023.02.017
摘要221)      PDF    收藏

株高是影响甘蓝型油菜产量、收获指数和抗倒伏性的关键株型特征,然而,油菜株高的遗传调控机制仍不清楚。本研究利用EMS诱变获得了一个半矮杆突变体df34遗传分析结果表明,df34半矮杆性状由一半显性基因控制。利用BSA-Seq方法将目的基因定位到C3染色体上命名为BnaSD.C3。随后,利用图位克隆的方法,将BnaSD.C3精细定位到“Darmor-bzh基因组的297.35 kb区间内。然而,在“Darmor-bzh基因组上的这一区间内,没有潜在的调控株高性状候选基因结合基因组重测序、转录组测序、植物激素分析、结构变异分析和基因功能注释等信息,在“ZS11”参考基因组上,发现BnaC03G0466900ZSBnaC03G0478900ZSBnaSD.C3的重要候选基因。研究为甘蓝型油菜矮化及株型育种提供了新的基因资源,为解析甘蓝型油菜株高的遗传调控机制提供了新的见解。

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5. Selection and characterisation of lactic acid bacteria isolated from different origins for ensiling Robinia pseudoacacia and Morus alba L. leaves
NI Kui-kui, YANG Hui-xiao, HUA Wei, WANG Yan-ping, PANG Hui-li
Journal of Integrative Agriculture    2016, 15 (10): 2353-2362.   DOI: 10.1016/S2095-3119(15)61251-5
摘要2160)      PDF    收藏
    The objective of this study was to isolate lactic acid bacteria (LAB) strains from different origins and to select the best strains for ensiling Robinia pseudoacacia (RB) and Morus alba L. (MB) leaves. The LAB strains were inoculated into the extracted liquid obtained from RB and MB leaves to evaluate the fermentation products. 11 LAB strains were selected for further experiments based on the highest products of lactic or acetic acid, including 1 strain of Weissella confusa, 2 of Lactobacillus reuteri and 8 of Lactobacillus plantarum. The API 50 CH fermentation experiment indicated that all of the selected 11 LAB strains utilised most of the carbohydrates. All the strains grew at temperatures between 10 and 45°C and at a pH of 3.5 to 4.5; however, L. reuteri F7 and F8 tolerated a pH as low as 3.0. All 11 LAB strains showed antibacterial activity against Listeria monocytogens, Escherichia coil, Salmonella sp. and Acetobacter pasteurianus; however, after excluding the effect of organic acids, only F7 and F8 still exhibited antibacterial activity. The present study indicated that the selected 11 LAB strains could be used to prepare silages of RB and MB leaves, especially L. reuteri F7 and F8.
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6. A Simple Method for Isolating Chloroplast DNA and Mitochondria DNA from the Same Rapeseed Green Leaf Tissue
HU Zhi-yong, ZHAN Gao-miao, WANG Han-zhong, HUA Wei
Journal of Integrative Agriculture    2012, 12 (7): 1212-1215.   DOI: 10.1016/S1671-2927(00)8649
摘要1486)      PDF    收藏
In the study, we present a fast, simple and inexpensive protocol for isolating chloroplast and mitochondrial DNA from one rapeseed leaf tissue sample. The chloroplast and mitochondria were separated from the same green leaf tissue by differential centrifugations. The protocol is the first report that isolates plant chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) from the same sample homogenate. The organelle DNA yield is 2-10 micrograms per gram of tissue; the DNA was fully restrictable and was successfully used for sequencing.
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