时序转录组分析揭示了西瓜对低温胁迫的响应

doi:10.1016/j.jia.2024.11.025

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (5): 1786-1799.DOI: 10.1016/j.jia.2024.11.025

时序转录组分析揭示了西瓜对低温胁迫的响应

收稿日期:2023-12-25 修回日期:2024-11-12 接受日期:2024-06-17 出版日期:2025-05-20 发布日期:2025-04-14

A time-course transcriptome reveals the response of watermelon to low-temperature stress

Jin Wang^{1, 2*}, Minghua Wei^1*, Haiyan Wang¹, Changjuan Mo¹, Yingchun Zhu^3#, Qiusheng Kong^1#

¹National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops/College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
²Engineering Research Center for Germplasm Innovation and New Varieties Breeding of Horticultural Crops/Key Laboratory for Vegetable Biology of Hunan Province/College of Horticulture, Hunan Agricultural University, Changsha 410128, China
³Institute of Western Agriculture, Chinese Academy of Agricultural Sciences, Changji 831100, China

Received:2023-12-25 Revised:2024-11-12 Accepted:2024-06-17 Online:2025-05-20 Published:2025-04-14
About author:Jin Wang, E-mail: jinwang214@163.com; Minghua Wei, E-mail: 2020305110101@webmail.hzau.edu.cn; #Correspondence Yingchun Zhu, E-mail: zhuyingchun@caas.cn; Qiusheng Kong, E-mail: qskong@mail.hzau.edu.cn * These authors contributed equally to this study.
Supported by:
This work was financed by the National Natural Science Foundation of China (31471894), the China Agriculture Research System of MOF and MARA (CARS-25), the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-ZFRI).

摘要/Abstract

摘要：

西瓜（Citrullus lanatus）是一种重要的园艺作物，但其易受低温胁迫的影响，这对西瓜生产和供应提出了重大挑战。尽管西瓜具有重要的经济价值，但对其在转录水平上对低温胁迫的响应知之甚少。在本研究中，我们进行了一个时序转录组分析，系统地研究了西瓜在低温胁迫下的调控网络。共鉴定出6个低温响应基因簇，代表6种表达模式，揭示了低温胁迫下西瓜代谢途径的多样性调控。对时间特异性差异表达基因的分析揭示了西瓜对低温响应的时间依赖性。此外，ClMYB14-OE过表达株系更易受到低温胁迫的影响，因此ClMYB14被发现是低温耐受性的负调控因子。共表达网络分析表明，ClMYB14通过调控不饱和脂肪酸途径和热激转录因子参与低温响应。本研究为了解西瓜响应低温胁迫的调控网络提供了重要信息，并为提高西瓜耐低温性的遗传改良提供了候选基因。

Abstract:

Watermelon (Citrullus lanatus) is an economically important horticultural crop. However, it is susceptible to low-temperature stress, which significantly challenges its production and supply. Despite the great economic importance of watermelon, little is known about its response to low-temperature stress at the transcriptional level. In this study, we performed a time-course transcriptome analysis to systematically investigate the regulatory network of watermelon under low-temperature stress. Six low-temperature-responsive gene clusters representing six expression patterns were identified, revealing diverse regulation of metabolic pathways in watermelon under low-temperature stress. Analysis of temporally specific differentially expressed genes revealed the time-dependent nature of the watermelon response to low temperature. Moreover, ClMYB14 was found to be a negative regulator of low-temperature tolerance as ClMYB14-OE lines were more susceptible to low-temperature stress. Co-expression network analysis demonstrated that ClMYB14 participates in the low-temperature response by regulating the unsaturated fatty acid pathway and heat shock transcription factor. This study provides substantial information for understanding the regulatory network of watermelon in response to low-temperature stress, and identifies candidate genes for the genetic improvement of watermelon with higher low-temperature tolerance.

Key words: watermelon , time-course transcriptome , low temperature , ClMYB14 , co-expression

Jin Wang, Minghua Wei, Haiyan Wang, Changjuan Mo, Yingchun Zhu, Qiusheng Kong. 时序转录组分析揭示了西瓜对低温胁迫的响应[J]. Journal of Integrative Agriculture, 2025, 24(5): 1786-1799.

Jin Wang, Minghua Wei, Haiyan Wang, Changjuan Mo, Yingchun Zhu, Qiusheng Kong. A time-course transcriptome reveals the response of watermelon to low-temperature stress[J]. Journal of Integrative Agriculture, 2025, 24(5): 1786-1799.

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时序转录组分析揭示了西瓜对低温胁迫的响应

A time-course transcriptome reveals the response of watermelon to low-temperature stress

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